1887

Abstract

The alphaproteobacterial strains GRP21 and PH34, which were isolated from coastal sediment of the East Sea, Korea, were subjected to a polyphasic taxonomic investigation. The strains were Gram-negative, non-motile, non-spore-forming, oval-shaped rods that produced creamy-white colonies on tryptic soy agar, required NaCl for growth, contained Q-10 as the predominant ubiquinone, contained 16 : 0, 18 : 17 and 19 : 0 cyclo 8 as major fatty acids and had polar lipid profiles consisting of phosphatidylcholine, phosphatidylglycerol, an unknown aminolipid, an unknown phospholipid and three unknown lipids. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that the strains were most closely related to KCTC 12735, with 94.5 % sequence similarity, but formed a separate lineage within the family . The combined genotypic and phenotypic data supported the conclusion that the strains represent a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is GRP21 (=KCTC 22497 =DSM 21219).

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2010-09-01
2020-01-22
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Two-dimensional TLC of polar lipids from strain GRP21 (a) and SW-277 (b) stained with molybdatophosphoric acid (Sigma). Solvents were as follows: first direction, chloroform/methanol/water (65:25:4 by vol.); second direction, chloroform/methanol/acetic acid/water (80:12:15:4 by vol.). PC, Phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; AL, unknown aminolipid; PL, unknown phospholipid; L1–3, unknown polar lipids that were not stainable with any of the specific spray reagents applied to detect a phosphate group, an amino group or a sugar moiety.

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Transmission electron micrograph of cells of strain GRP21 . Bar, 1 µm.

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Phylogenetic tree based on 16S rRNA gene sequences, constructed according to the neighbour-joining method, showing the positions of strains GRP21 and PH34 among representative species within the family . [PDF](49 KB)

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