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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 60, Issue 11

sp. nov., a facultative methanotroph possessing a particulate methane monooxygenase, and emended description of the genus Free

Abstract

An aerobic, methanotrophic bacterium, designated KYG, was isolated from a forest soil in Germany. Cells of strain KYG were Gram-negative, non-motile, slightly curved rods that multiplied by binary fission and produced yellow colonies. The cells contained intracellular granules of poly--hydroxybutyrate at each cell pole, a particulate methane monooxygenase (pMMO) and stacks of intracytoplasmic membranes (ICMs) packed in parallel along one side of the cell envelope. Strain KYG grew at pH 5.2–7.2 and 2–33 °C and could fix atmospheric nitrogen under reduced oxygen tension. The major cellular fatty acid was C 7 (81.5 %) and the DNA G+C content was 61.4 mol%. Strain KYG belonged to the family of the class and was most closely related to the obligate methanotroph B2 (98.1 % 16S rRNA gene sequence similarity and 84.7 % sequence similarity). Unlike B2, which grows only on methane and methanol, strain KYG was able to grow facultatively on acetate. Facultative acetate utilization is a characteristic of the methanotrophs of the genus , but the genus does not produce pMMO or ICMs. Strain KYG differed from B2 on the basis of substrate utilization pattern, pigmentation, pH range, cell ultrastructure and efficiency of dinitrogen fixation. Therefore, we propose a novel species, sp. nov., to accommodate this bacterium. The type strain is KYG (=DSM 22158 =VKM B-2544).

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Keyword(s): ICM, intracytoplasmic membrane and pMMO, particulate methane monooxygenase; sMMO, soluble methane monooxygenase
SGM
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2010-11-01
2024-04-18
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Methylocapsa aurea sp. nov., a facultative methanotroph possessing a particulate methane monooxygenase, and emended description of the genus Methylocapsa
Int J Syst Evol Microbiol 60, 2659 (2010); https://doi.org/10.1099/ijs.0.020149-0
/content/journal/ijsem/10.1099/ijs.0.020149-0
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