1887

Abstract

A mesophilic, hydrogenotrophic methanogen, designated strain Ki8-1, was isolated from soil. Cells were strictly anaerobic, Gram-stain-negative, non-sporulating, motile by means of a single flagellum or tufted flagella, and curved or wavy rod-shaped (11–25 μm long). The temperature and pH for optimum growth were 30 °C and 7.5. The strain grew best in basal medium without the addition of NaCl. Methane was produced from H and formate. Acetate or yeast extract was required for growth. The G+C content of the genomic DNA of strain Ki8-1 was 45.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Ki8-1 was a member of the genus and showed 95.1 % sequence similarity to NBRC 100397. On the basis of its phenotypic characteristics and phylogenetic position, strain Ki8-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Ki8-1 (NBRC 104920 =JCM 16384 =DSM 22751). Emended descriptions of the genus and of are also provided.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.020131-0
2010-11-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/11/2563.html?itemId=/content/journal/ijsem/10.1099/ijs.0.020131-0&mimeType=html&fmt=ahah

References

  1. Adachi, J. & Hasegawa, M. ( 1995; ). Improved dating of the human chimpanzee separation in the mitochondrial-DNA tree: heterogeneity among amino-acid sites. J Mol Evol 40, 622–628.[CrossRef]
    [Google Scholar]
  2. Boone, D. R., Whitman, W. B. & Koga, Y. ( 2001; ). Family III. Methanospirillaceae fam. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 264–267. Edited by Boone, D. R. & Castenholz, R. W.. New York. : Springer.
    [Google Scholar]
  3. Bräuer, S. L., Cadillo-Quiroz, H., Yadhiro, E., Yavitt, J. B. & Zinder, S. H. ( 2006; ). Isolation of a novel acidiphilic methanogen from an acidic peat bog. Nature 442, 192–194.[CrossRef]
    [Google Scholar]
  4. Ferry, J. G., Smith, P. H. & Wolfe, R. S. ( 1974; ). Methanospirillum, a new genus of methanogenic bacteria, and characterization of Methanospirillum hungatii sp. nov. Int J Syst Bacteriol 24, 465–469.[CrossRef]
    [Google Scholar]
  5. Garrity, G. M. & Holt, J. G. ( 2001; ). Phylum AII. Euryarchaeota phy. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 211–213. Edited by Boone, D. R. & Castenholz, R. W.. New York. : Springer.
    [Google Scholar]
  6. Hales, B. A., Edwards, C., Ritchie, D. A., Hall, G., Pickup, R. W. & Saunders, J. R. ( 1996; ). Isolation and identification of methanogen-specific DNA from blanket bog peat by PCR amplification and sequence analysis. Appl Environ Microbiol 62, 668–675.
    [Google Scholar]
  7. Jones, W. J., Nagle, D. P., Jr & Whitman, W. B. ( 1987; ). Methanogens and the diversity of archaebacteria. Microbiol Rev 51, 135–177.
    [Google Scholar]
  8. Joye, S. B., Samarkin, V. A., Orcutt, B. N., MacDonald, I. R., Hinrichs, K.-U., Elvert, M., Teske, A. P., Lloyd, K. G., Lever, M. A. & other authors ( 2009; ). Metabolic variability in seafloor brines revealed by carbon and sulphur dynamics. Nature Geosci 2, 349–354.[CrossRef]
    [Google Scholar]
  9. Liu, Y. & Whitman, W. B. ( 2008; ). Metabolic, phylogenetic, and ecological diversity of the methanogenic archaea. Ann N Y Acad Sci 1125, 171–189.[CrossRef]
    [Google Scholar]
  10. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  11. Narihiro, T., Terada, T., Ohashi, A., Wu, J.-H., Liu, W.-T., Araki, N., Kamagata, Y., Nakamura, K. & Sekiguchi, Y. ( 2009; ). Quantitative detection of culturable methanogenic archaea abundance in anaerobic treatment systems using the sequence-specific rRNA cleavage method. ISME J 3, 522–535.[CrossRef]
    [Google Scholar]
  12. Patel, G. B., Roth, L. A., van den Berg, L. & Clark, D. S. ( 1976; ). Characterization of a strain of Methanospirillum hungatii. Can J Microbiol 22, 1404–1410.[CrossRef]
    [Google Scholar]
  13. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  14. Simankova, M. V., Kotsyurbenko, O. R., Lueders, T., Nozhevnikova, A. N., Wagner, B., Conrad, R. & Friedrich, M. W. ( 2003; ). Isolation and characterization of new strains of methanogens from cold terrestrial habitats. Syst Appl Microbiol 26, 312–318.[CrossRef]
    [Google Scholar]
  15. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  16. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  17. Touzel, J. P. & Albagnac, G. ( 1983; ). Isolation and characterization of Methanococcus mazei strain MC3. FEMS Microbiol Lett 16, 241–245.[CrossRef]
    [Google Scholar]
  18. Wolin, E. A., Wolin, M. J. & Wolfe, R. S. ( 1963; ). Formation of methane by bacterial extracts. J Biol Chem 238, 2882–2886.
    [Google Scholar]
  19. Zengler, K., Richnow, H. H., Rosselló-Mora, R., Michaelis, W. & Widdel, F. ( 1999; ). Methane formation from long-chain alkanes by anaerobic microorganisms. Nature 401, 266–269.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.020131-0
Loading
/content/journal/ijsem/10.1099/ijs.0.020131-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error