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Volume 60, Issue 10

sp. nov., isolated from an indoor wall Free

Abstract

A Gram-positive, coccoid, non-endospore-forming actinobacterium (strain 12-Be-011) was isolated from indoor wall material. Based on 16S rRNA gene sequence comparisons, strain 12-Be-011 was clearly shown to belong to the genus and was most closely related to Gsoil 954 (95.7 %), CC-12602 (94.9 %), Gsoil 633 (94.8 %), YIM 45721 (95.5 %) and DSM 10555 (94.7 %). The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. Mycolic acids were absent. The major menaquinone was MK-9(H). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unknown phospholipids and one unknown glycolipid. The major fatty acids of iso-C, anteiso-C and iso-C supported the affiliation of strain 12-Be-011 to the genus . Physiological and biochemical test results allowed a clear phenotypic differentiation of strain 12-Be-011 from all other species of the genus . Hence, strain 12-Be-011 can be regarded as a representative of a novel species, for which the name sp. nov. is proposed, with the type strain 12-Be-011 (=DSM 22083=CCM 7636).

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Keyword(s): ll-Dpm, ll-diaminopimelic acid
SGM
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2010-10-01
2024-04-25
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References

  1. An D.-S., Im W.-T., Yoon M.-H. 2008; Microlunatus panaciterrae sp. nov., a β -glucosidase-producing bacterium, isolated from soil in a ginseng field. Int J Syst Evol Microbiol 58:2734–2738 [CrossRef]
     [Google Scholar]
  2. Becker B., Lechevalier M. P., Lechevalier H. A. 1965; Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 13:236–243
     [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
     [Google Scholar]
  4. Cui Y. S., Im W. T., Yin C. R., Yang D. C., Lee S. T. 2007; Microlunatus ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 57:713–716 [CrossRef]
     [Google Scholar]
  5. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov. a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
     [Google Scholar]
  6. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
     [Google Scholar]
  7. Kämpfer P., Kroppenstedt R. M. 2004; Pseudonocardia benzenivorans sp. nov. Int J Syst Evol Microbiol 54:749–751 [CrossRef]
     [Google Scholar]
  8. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [CrossRef]
     [Google Scholar]
  9. Kämpfer P., Young C.-C., Busse H.-J., Chu J.-N., Schumann P., Arun A. B., Shen F.-T., Rekha P. D. 2010; Microlunatus soli , isolated from soil. Int J Syst Evol Microbiol 60:824–827 [CrossRef]
     [Google Scholar]
  10. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Kumar Y., Buchner A., Lai T., Steppi S. other authors 2004; arb: A software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
     [Google Scholar]
  11. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium , Nocardia , and related taxa by thin-layer chromatographic analysis of whole organism methanolysates. J Gen Microbiol 88:200–204 [CrossRef]
     [Google Scholar]
  12. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas , Oerskovia and related taxa. J Appl Bacteriol 47:87–95 [CrossRef]
     [Google Scholar]
  13. Nakamura K., Hiraishi A., Yoshimi Y., Kawaharasaki M., Masuda K., Kamagata Y. 1995; Microlunatus phosphovorus gen. nov., sp. nov. a new gram-positive polyphosphate-accumulating bacterium isolated from activated sludge. Int J Syst Bacteriol 45:17–22 [CrossRef]
     [Google Scholar]
  14. Olsen G. J., Matsuda H., Hagstrom R., Overbeek R. 1994; fastDNAml: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci 10:41–48
     [Google Scholar]
  15. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. 2007; silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb. Nucleic Acids Res 35:7188–7196 [CrossRef]
     [Google Scholar]
  16. Schäfer J., Martin K., Kämpfer P. 2010; Prauserella muralis sp. nov., from an indoor environment. Int J Syst Evol Microbiol 60:287–290 [CrossRef]
     [Google Scholar]
  17. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
     [Google Scholar]
  18. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
     [Google Scholar]
  19. Wang Y.-X., Cai M., Zhi X.-Y., Zhang Y.-Q., Tang S.-K., Yu L.-H., Cui X.-L., Li W.-J. 2008; Microlunatus aurantiacus sp. nov., a novel actinobacterium isolated from a rhizosphere soil sample. Int J Syst Evol Microbiol 58:1873–1877 [CrossRef]
     [Google Scholar]
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Microlunatus parietis sp. nov., isolated from an indoor wall
Int J Syst Evol Microbiol 60, 2420 (2010); https://doi.org/10.1099/ijs.0.020115-0
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