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Abstract

A Gram-positive, coccoid, non-endospore-forming actinobacterium (strain 12-Be-011) was isolated from indoor wall material. Based on 16S rRNA gene sequence comparisons, strain 12-Be-011 was clearly shown to belong to the genus and was most closely related to Gsoil 954 (95.7 %), CC-12602 (94.9 %), Gsoil 633 (94.8 %), YIM 45721 (95.5 %) and DSM 10555 (94.7 %). The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. Mycolic acids were absent. The major menaquinone was MK-9(H). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unknown phospholipids and one unknown glycolipid. The major fatty acids of iso-C, anteiso-C and iso-C supported the affiliation of strain 12-Be-011 to the genus . Physiological and biochemical test results allowed a clear phenotypic differentiation of strain 12-Be-011 from all other species of the genus . Hence, strain 12-Be-011 can be regarded as a representative of a novel species, for which the name sp. nov. is proposed, with the type strain 12-Be-011 (=DSM 22083=CCM 7636).

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2010-10-01
2019-12-13
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Two-dimensional TLC of polar lipid extracts from strain 12-Be-011 , stained with molybdatophosphoric acid. DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PI, phosphatidylinositol; GL, unknown glycolipid; PL1 and PL2, unknown phospholipids.

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Major fatty acid compositions (%) of the type strains of species of the genus . [ PDF] 38 KB

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