RT Journal Article SR Electronic(1) A1 Qin, Sheng A1 Zhu, Wen-Yong A1 Jiang, Ji-Hong A1 Klenk, Hans-Peter A1 Li, Jie A1 Zhao, Guo-Zhen A1 Xu, Li-Hua A1 Li, Wen-JunYR 2010 T1 Pseudonocardia tropica sp. nov., an endophytic actinomycete isolated from the stem of Maytenus austroyunnanensis JF International Journal of Systematic and Evolutionary Microbiology, VO 60 IS 11 SP 2524 OP 2528 DO https://doi.org/10.1099/ijs.0.020099-0 PB Microbiology Society, SN 1466-5034, AB A novel strain, designated YIM 61452T, was isolated from the stem of Maytenus austroyunnanensis collected from the tropical rainforest of Xishuangbanna, Yunnan Province, south-west China. The strain was characterized taxonomically by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 61452T belonged to the genus Pseudonocardia and was most closely related to Pseudonocardia alni IMSNU 20049T (99.5 %), Pseudonocardia antarctica DVS 5a1T (99.5 %) and Pseudonocardia carboxydivorans Y8T (99.4 %). The chemotaxonomic properties of strain YIM 61452T, for example MK-8(H4) as the predominant quinone system and iso-C16 : 0 and iso-C16 : 1 H as the major fatty acids, were also consistent with its classification within the genus Pseudonocardia. Strain YIM 61452T could be differentiated from closely related species of the genus Pseudonocardia by phenotypic and genotypic analysis. DNA–DNA hybridization experiments between strain YIM 61452T and closely related reference strains further confirmed that strain YIM 61452T represented a novel taxon of the genus Pseudonocardia. Therefore, strain YIM 61452T represents a novel species, for which the name Pseudonocardia tropica sp. nov. is proposed. The type strain is YIM 61452T (=DSM 45199T=CCTCC AA 208018T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.020099-0