1887

Abstract

Four novel isolates (R-40508, R-40507, R-40903 and R-21419) were obtained from different cnidarian species (, and ) from different places in Brazil and Australia. The novel isolates formed a tight phylogenetic group based on 16S rRNA, , , , and gene sequences. Their closest phylogenetic neighbours were the type strains of , and , sharing 97.1–97.5 % 16S rRNA gene sequence similarity. DNA–DNA hybridization between a representative strain (R-40508) and the type strains of these species revealed less than 20 % relatedness, showing that the new isolates belong to a novel species. Several phenotypic features allow the differentiation of the novel species from its closest phylogenetic neighbours. It has gelatinase and lipase activity and can utilize melibiose, but it cannot grow on 6 % NaCl. In addition, the novel species has the fatty acid iso-C, but lacks the fatty acids C, C cyclo, iso-C, C 8 and iso-C 9. The name sp. nov. is proposed for this species, with the type strain R-40508 (=LMG 25436 =CAIM 1817). The G+C content of the type strain is 45.5 mol%.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.019968-0
2010-12-01
2020-01-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/12/2843.html?itemId=/content/journal/ijsem/10.1099/ijs.0.019968-0&mimeType=html&fmt=ahah

References

  1. Alves, N., Jr, Maia Neto, O. S., Silva, B. S. O., De Moura, R. L., Francini-Filho, R. B., Barreira e Castro, C., Paranhos, R., Bitner-Mathé, B. C., Kruger, R. H. & other authors ( 2010; ). Diversity and pathogenic potential of vibrios isolated from Abrolhos Bank corals. Environ Microbiol Rep 2, 90–95.
    [Google Scholar]
  2. Ast, J. C., Cleenwerck, I., Engelbeen, K., Urbanczyk, H., Thompson, F. L., De Vos, P. & Dunlap, P. V. ( 2007; ). Photobacterium kishitanii sp. nov., a luminous marine bacterium symbiotic with deep-sea fishes. Int J Syst Evol Microbiol 57, 2073–2078.[CrossRef]
    [Google Scholar]
  3. Baumann, P. & Baumann, L. ( 1984; ). Genus Photobacterium Beijerinck 1889, 401AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 539–545. Edited by Krieg, N. R. & Holt, J. G.. Baltimore. : Williams & Wilkins.
    [Google Scholar]
  4. Chimetto, L. A., Brocchi, M., Thompson, C. C., Martins, R. C. R., Ramos, H. B. & Thompson, F. L. ( 2008; ). Vibrios dominate as culturable nitrogen-fixing bacteria of Brazilian coral Mussismulia hispida. Syst Appl Microbiol 31, 312–319.[CrossRef]
    [Google Scholar]
  5. Chimetto, L. A., Brocchi, M., Gondo, M., Thompson, C. C., Gomez-Gil, B. & Thompson, F. L. ( 2009; ). Genomic diversity of vibrios associated with the Brazilian coral Mussismilia hispida and its sympatric zoanthids (Palythoa caribaeorum, Palythoa variabilis and Zoanthus solanderi). J Appl Microbiol 106, 1818–1826.[CrossRef]
    [Google Scholar]
  6. Dinsdale, E. A., Pantos, O., Smriga, S., Edwards, R. A., Angly, F., Wegley, L., Hatay, M., Hall, D., Brown, E. & other authors ( 2008; ). Microbial ecology of four coral atolls in the Northern Line Islands. PLoS One 3, e1584.[CrossRef]
    [Google Scholar]
  7. Ducklow, H. W. & Mitchell, R. ( 1979; ). Composition of mucus released by coral reef coelenterates. Limnol Oceanogr 24, 706–714.[CrossRef]
    [Google Scholar]
  8. Eck, R. V. & Dayhoff, M. O. ( 1966; ). Atlas of Protein Sequence and Structure. Silver Springs, MD. : National Biomedical Research Foundation.
    [Google Scholar]
  9. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  10. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  11. Gomez-Gil, B., Roque, A., Rotllant, G., Peinado, L., Romalde, J. L., Doce, A., Cabanillas-Beltrán, H., Chimetto, L. & Thompson, F. L. ( 2011; ). Photobacterium swingsii sp. nov., isolated from marine organisms. Int J Syst Evol Microbiol 61 (in press). doi:10.1099/ijs.0.019687-0
    [Google Scholar]
  12. Goris, J., Suzuki, K., De Vos, P., Nakase, T. & Kersters, K. ( 1998; ). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef]
    [Google Scholar]
  13. Huys, G., Vancanneyt, M., Coopman, R., Janssen, P., Falsen, E., Altwegg, M. & Kersters, K. ( 1994; ). Cellular fatty-acid composition as a chemotaxonomic marker for the differentiation of phenospecies and hybridization groups in the genus Aeromonas. Int J Syst Bacteriol 44, 651–658.[CrossRef]
    [Google Scholar]
  14. Jung, S. Y., Jung, Y. T., Oh, T. K. & Yoon, J. H. ( 2007; ). Photobacterium lutimaris sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 57, 332–336.[CrossRef]
    [Google Scholar]
  15. Kooperman, N., Ben-Dov, E., Kramarsky-Winter, E., Barak, Z. & Kushmaro, A. ( 2007; ). Coral mucus-associated bacterial communities from natural and aquarium environments. FEMS Microbiol Lett 276, 106–113.[CrossRef]
    [Google Scholar]
  16. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  17. Nogi, Y., Masui, N. & Kato, C. ( 1998; ). Photobacterium profundum sp. nov., a new, moderately barophilic bacterial species isolated from a deep-sea sediment. Extremophiles 2, 1–7.[CrossRef]
    [Google Scholar]
  18. Park, Y. D., Baik, K. S., Seong, C. N., Bae, K. S., Kim, S. & Chun, J. ( 2006; ). Photobacterium ganghwense sp. nov., a halophilic bacterium isolated from sea water. Int J Syst Evol Microbiol 56, 745–749.[CrossRef]
    [Google Scholar]
  19. Ritchie, K. B. ( 2006; ). Regulation of microbial populations by coral surface mucus and mucus-associated bacteria. Mar Ecol Prog Ser 322, 1–14.[CrossRef]
    [Google Scholar]
  20. Rivas, R., Garcia-Fraile, P., Mateos, P. F., Martinez-Molina, E. & Velázquez, E. ( 2006; ). Photobacterium halotolerans sp. nov., isolated from Lake Martel in Spain. Int J Syst Evol Microbiol 56, 1067–1071.[CrossRef]
    [Google Scholar]
  21. Rohwer, F., Breitbart, M., Jara, J., Azam, F. & Knowlton, N. ( 2001; ). Diversity of bacteria associated with the Caribbean coral Montastraea franksi. Coral Reefs 20, 85–91.[CrossRef]
    [Google Scholar]
  22. Rosenberg, E., Koren, O., Reshef, L., Efrony, R. & Zilber-Rosenberg, I. ( 2007; ). The role of microorganisms in coral health, disease and evolution. Nat Rev Microbiol 5, 355–362.[CrossRef]
    [Google Scholar]
  23. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  24. Sawabe, T., Kita-Tsukamoto, K. & Thompson, F. L. ( 2007; ). Inferring the evolutionary history of vibrios by means of multilocus sequence analysis. J Bacteriol 189, 7932–7936.[CrossRef]
    [Google Scholar]
  25. Seo, H. J., Bae, S. S., Lee, J. H. & Kim, S. J. ( 2005; ). Photobacterium frigidiphilum sp. nov., a psychrophilic, lipolytic bacterium isolated from deep-sea sediments of Edison Seamount. Int J Syst Evol Microbiol 55, 1661–1666.[CrossRef]
    [Google Scholar]
  26. Shnit-Orland, M. & Kushmaro, A. ( 2009; ). Coral mucus-associated bacteria: a possible first line of defense. FEMS Microbiol Ecol 67, 371–380.[CrossRef]
    [Google Scholar]
  27. Sussman, M., Mieog, J. C., Doyle, J., Victor, S., Willis, B. L. & Bourne, D. G. ( 2009; ). Vibrio zinc-metalloprotease causes photoinactivation of coral endosymbionts and coral tissue lesions. PLoS One 4, e4511.[CrossRef]
    [Google Scholar]
  28. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  29. Thompson, F. L., Hoste, B., Thompson, C. C., Huys, G. & Swings, J. ( 2001a; ). The coral bleaching Vibrio shiloi Kushmaro et al. 2001 is a later synonym of Vibrio mediterranei Pujalte and Garay 1986. Syst Appl Microbiol 24, 516–519.[CrossRef]
    [Google Scholar]
  30. Thompson, F. L., Hoste, B., Vandemeulebroecke, K. & Swings, J. ( 2001b; ). Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplified fragment length polymorphism. Syst Appl Microbiol 24, 520–538.[CrossRef]
    [Google Scholar]
  31. Thompson, F. L., Thompson, C. C., Naser, S., Vandemeulebroucke, K., Hoste, B., Munn, C. B., Bourne, D. & Swings, J. ( 2005a; ). Photobacterium rosenbergii sp. nov. and Enterovibrio coralii sp. nov., vibrios associated with coral bleaching. Int J Syst Evol Microbiol 55, 913–917.[CrossRef]
    [Google Scholar]
  32. Thompson, F. L., Gevers, D., Thompson, C. C., Dawyndt, P., Naser, S., Hoste, B., Munn, C. B. & Swings, J. ( 2005b; ). Phylogeny and molecular identification of vibrios based on multilocus sequence analysis. Appl Environ Microbiol 71, 5107–5115.[CrossRef]
    [Google Scholar]
  33. Thompson, F. L., Barash, Y., Sawabe, T., Sharon, G., Swings, J. & Rosenberg, E. ( 2006; ). Thalassomonas loyana sp. nov., a causative agent of the white plague-like disease of corals on the Eilat coral reef. Int J Syst Evol Microbiol 56, 365–368.[CrossRef]
    [Google Scholar]
  34. Willems, A., Doignon-Bourcier, F., Goris, J., Coopman, R., de Lajudie, P., De Vos, P. & Gillis, M. ( 2001; ). DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51, 1315–1322.
    [Google Scholar]
  35. Xie, C.-H. & Yokota, A. ( 2004; ). Transfer of Hyphomicrobium indicum to the genus Photobacterium as Photobacterium indicum comb. nov. Int J Syst Evol Microbiol 54, 2113–2116.[CrossRef]
    [Google Scholar]
  36. Yoon, J. H., Lee, J. K., Kim, Y. O. & Oh, T. K. ( 2005; ). Photobacterium lipolyticum sp. nov., a bacterium with lipolytic activity isolated from the Yellow Sea in Korea. Int J Syst Evol Microbiol 55, 335–339.[CrossRef]
    [Google Scholar]
  37. Yoshizawa, S., Wada, M., Kita-Tsukamoto, K., Yokota, A. & Kogure, K. ( 2009; ). Photobacterium aquimaris sp. nov., a luminous marine bacterium isolated from seawater. Int J Syst Evol Microbiol 59, 1438–1442.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.019968-0
Loading
/content/journal/ijsem/10.1099/ijs.0.019968-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 2843 - 2848

GenBank/EMBL/DDBJ accession numbers of the 16S rRNA and housekeeping gene sequences of strains of sp. nov.

Phenotypic variability amongst strains of sp. nov.

[PDF file of Supplementary Tables](41 KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error