sp. nov., isolated from a wound infection caused by a dog bite Free

Abstract

A non-lipophilic, coryneform bacterium isolated from a patient's wound caused by a dog bite was characterized by phenotypic, chemotaxonomic and molecular genetic methods. Chemotaxonomic features suggested assignment of the unknown bacterium to the genus . The isolate exhibited the following unusual features, which made it possible to phenotypically differentiate it from all other medically relevant corynebacteria: the Gram stain showed some very filamentous rods (>15 μm in length); some cells exhibited branching; colonies were domed and adherent to agar; the micro-organism was positive for pyrazinamidase, -glucosidase, -glucosidase and trypsin but negative for -galactosidase. 16S rRNA gene sequencing and partial gene sequencing showed that the closest phylogenetic relative, , exhibited more than 1.9 % and 17.9 % divergence with the unknown bacterium, respectively. Based on both phenotypic and molecular genetic data, it is proposed that the isolate should be classified as a novel species, sp. nov., with the type strain 1170 (=CCUG 58627 =DSM 45402).

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2010-11-01
2024-03-29
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References

  1. Beck M., Frodl R., Funke G. 2008; Comprehensive study of strains previously designated Streptococcus bovis consecutively isolated from human blood cultures and emended description of Streptococcus gallolyticus and Streptococcus infantarius subsp. coli . J Clin Microbiol 46:2966–2972 [CrossRef]
    [Google Scholar]
  2. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  3. CLSI 2006 Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria , document M45-A Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  4. Collins M. D., Bernard K. A., Hutson R. A., Sjödén B., Nyberg A., Falsen E. 1999a; Corynebacterium sundsvallense sp. nov., from human clinical specimens. Int J Syst Bacteriol 49:361–366 [CrossRef]
    [Google Scholar]
  5. Collins M. D., Hoyles L., Lawson P. A., Falsen E., Robson R. L., Foster G. 1999b; Phenotypic and phylogenetic characterization of a new Corynebacterium species from dogs: description of Corynebacterium auriscanis sp. nov. J Clin Microbiol 37:3443–3447
    [Google Scholar]
  6. Collins M. D., Hoyles L., Foster G., Sjödén B., Falsen E. 2001a; Corynebacterium capitovis sp. nov., from a sheep. Int J Syst Evol Microbiol 51:857–860 [CrossRef]
    [Google Scholar]
  7. Collins M. D., Hoyles L., Hutson R. A., Foster G., Falsen E. 2001b; Corynebacterium testudinoris sp. nov., from a tortoise, and Corynebacterium felinum sp. nov., from a Scottish wild cat. Int J Syst Evol Microbiol 51:1349–1352
    [Google Scholar]
  8. Collins M. D., Hoyles L., Foster G., Falsen E. 2004; Corynebacterium caspium sp. nov., from a Caspian seal ( Phoca caspica ). Int J Syst Evol Microbiol 54:925–928 [CrossRef]
    [Google Scholar]
  9. Fernández-Garayzábal J. F., Egido R., Vela A. I., Briones V., Collins M. D., Mateos A., Hutson R. A., Domínguez L., Goyache J. 2003; Isolation of Corynebacterium falsenii and description of Corynebacterium aquilae sp. nov., from eagles. Int J Syst Evol Microbiol 53:1135–1138 [CrossRef]
    [Google Scholar]
  10. Fernández-Garayzábal J. F., Vela A. I., Egido R., Hutson R. A., Lanzarot M. P., Fernández-García M., Collins M. D. 2004; Corynebacterium ciconiae sp. nov., isolated from the trachea of black storks ( Ciconia nigra ). Int J Syst Evol Microbiol 54:2191–2195 [CrossRef]
    [Google Scholar]
  11. Funke G., Bernard K. A. 2007; Coryneform Gram-positive rods. In Manual of Clinical Microbiology, 9th edn. pp 485–514 Edited by Murray P. R., Baron E. J., Jorgensen J. H., Landry M. L., Pfaller M. A. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  12. Funke G., Frodl R. 2008; Comprehensive study of Corynebacterium freneyi strains and extended and emended description of Corynebacterium freneyi Renaud, Aubel, Riegel, Meugnier, and Bollet 2001. J Clin Microbiol 46:638–643 [CrossRef]
    [Google Scholar]
  13. Funke G., Martinetti Lucchini G., Pfyffer G. E., Marchiani M., von Graevenitz A. 1993; Characteristics of CDC group 1 and group 1-like coryneform bacteria isolated from clinical specimens. J Clin Microbiol 31:2907–2912
    [Google Scholar]
  14. Funke G., Lawson P. A., Bernard K. A., Collins M. D. 1996; Most Corynebacterium xerosis strains identified in the routine clinical laboratory correspond to Corynebacterium amycolatum . J Clin Microbiol 34:1124–1128
    [Google Scholar]
  15. Funke G., Frodl R., Bernard K. A., Englert R. 2009; Corynebacterium freiburgense sp. nov., isolated from a wound obtained from a dog bite. Int J Syst Evol Microbiol 59:2054–2057 [CrossRef]
    [Google Scholar]
  16. Funke G., Frodl R., Bernard K. A. 2010; Corynebacterium mustelae sp. nov., isolated from a ferret with lethal sepsis. Int J Syst Evol Microbiol 60:871–873 [CrossRef]
    [Google Scholar]
  17. Goyache J., Ballesteros C., Vela A. I., Collins M. D., Briones V., Hutson R. A., Potti J., García-Borboroglu P., Domínguez L., Fernández-Garayzábal J. F. 2003a; Corynebacterium sphenisci sp. nov., isolated from wild penguins. Int J Syst Evol Microbiol 53:1009–1012 [CrossRef]
    [Google Scholar]
  18. Goyache J., Vela A. I., Collins M. D., Ballesteros C., Briones V., Moreno J., Yorio P., Domínguez L., Hutson R., Fernández-Garayzábal J. F. 2003b; Corynebacterium spheniscorum sp. nov., isolated from the cloacae of wild penguins. Int J Syst Evol Microbiol 53:43–46 [CrossRef]
    [Google Scholar]
  19. Khamis A., Raoult D., La Scola B. 2004; rpoB gene sequencing for identification of Corynebacterium species. J Clin Microbiol 42:3925–3931 [CrossRef]
    [Google Scholar]
  20. Lartigue M.-F., Monnet X., Le Flèche A., Grimont P. A. D., Benet J.-J., Durrbach A., Fabre M., Nordmann P. 2005; Corynebacterium ulcerans in an immunocompromised patient with diphtheria and her dog. J Clin Microbiol 43:999–1001 [CrossRef]
    [Google Scholar]
  21. Nakao H., Popovic T. 1997; Development of a direct PCR assay for detection of the diphtheria toxin gene. J Clin Microbiol 35:1651–1655
    [Google Scholar]
  22. Riegel P., Heller R., Prevost G., Jehl F., Monteil H. 1997; Corynebacterium durum sp. nov., from human clinical specimens. Int J Syst Bacteriol 47:1107–1111 [CrossRef]
    [Google Scholar]
  23. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
    [Google Scholar]
  24. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  25. Vela A. I., Mateos A., Collins M. D., Briones V., Hutson R. A., Domínguez L., Fernández-Garayzábal J. F. 2003; Corynebacterium suicordis sp. nov., from pigs. Int J Syst Evol Microbiol 53:2027–2031 [CrossRef]
    [Google Scholar]
  26. Yassin A. F. 2009; Corynebacterium ulceribovis sp. nov., isolated from the skin of the udder of a cow with a profound ulceration. Int J Syst Evol Microbiol 59:34–37 [CrossRef]
    [Google Scholar]
  27. Zimmermann O., Spröer C., Kroppenstedt R. M., Fuchs E., Köchel H. G., Funke G. 1998; Corynebacterium thomssenii sp. nov., a Corynebacterium with N -acetyl- β -glucosaminidase activity from human clinical specimens. Int J Syst Bacteriol 48:489–494 [CrossRef]
    [Google Scholar]
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