1887

Abstract

A yellow-pigmented bacterial strain, R4-1A, isolated from the midgut of the mosquito (a vector of lymphatic filariasis), was studied using a polyphasic approach. Cells of the isolate were rod-shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequence of this organism with sequences of type strains of the most closely related species clearly showed an allocation to the genus , with the highest sequence similarities (all 97.9 %) to JS17-8, ATCC 29897, CC-VM-7 and CW9. 16S rRNA gene sequence similarities to type strains of other species were below 97.5 %. The fatty acid profile of strain R4-1A included the major fatty acids iso-15 : 0, summed feature 4 (comprising iso-15 : 0 2-OH and/or 16 : 17), iso-17 : 1 and iso-17 : 0 3-OH. DNA–DNA hybridizations with KACC 12501, CCUG 14556, CC-VM-7 and KCTC 12894 resulted in relatedness values of 38.3 % (reciprocal 30.5 %), 29.4 % (32.1 %), 23.2 % (37.2 %) and 29.5 % (47.1 %), respectively. These results and the differentiating biochemical and chemotaxonomic properties show that strain R4-1A represents a novel species, for which the name sp. nov. is proposed. The type strain is R4-1A (=LMG 25442 =CCM 7716).

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2010-10-01
2019-10-16
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