1887

Abstract

Six strains of anaerobic, pleomorphic Gram-positive bacilli, isolated from the human oral cavity and an infected arm wound, were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group. 16S rRNA gene sequence analysis revealed that the isolates were most closely related to CCUG 35729 (94.8–94.9 % 16S rRNA gene sequence similarity). The isolates were saccharolytic and produced acetic and lactic acids as end products of fermentation. The major fatty acids were C (49.8 %) and C 9 (35.8 %). Polar lipid analysis revealed a variety of glycolipids, diphosphatidylglycerol, an unidentified phospholipid and an unidentified phosphoglycolipid. No respiratory quinones were detected. The peptidoglycan was of the type A4 -Lys–Thr–Glu, with -lysine partially replaced by -ornithine. The DNA G+C content of one of the strains, C1A_55 was 55 mol%. A novel species, sp. nov., is proposed to accommodate the six isolates, with the type strain C1A_55 (=DSM 22547=CCUG 58090).

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2011-01-01
2019-12-12
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References

  1. Crociani, F., Biavati, B., Alessandrini, A., Chiarini, C. & Scardovi, V. ( 1996; ). Bifidobacterium inopinatum sp. nov. and Bifidobacterium denticolens sp. nov., two new species isolated from human dental caries. Int J Syst Bacteriol 46, 564–571.[CrossRef]
    [Google Scholar]
  2. Downes, J., Sutcliffe, I., Tanner, A. C. & Wade, W. G. ( 2005; ). Prevotella marshii sp. nov. and Prevotella baroniae sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol 55, 1551–1555.[CrossRef]
    [Google Scholar]
  3. Hall, T. ( 2004; ). BioEdit. Biological sequence alignment editor for Win95/98/NT/2K/XP. Carlsbad, CA: Ibis Biosciences.
  4. Holdeman, L. V. H., Cato, E. P. & Moore, W. E. C. ( 1977; ). Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA. : Virginia Polytechnic Institute and State University.
    [Google Scholar]
  5. Hooper, S. J., Crean, S. J., Lewis, M. A., Spratt, D. A., Wade, W. G. & Wilson, M. J. ( 2006; ). Viable bacteria present within oral squamous cell carcinoma tissue. J Clin Microbiol 44, 1719–1725.[CrossRef]
    [Google Scholar]
  6. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  7. Jian, W. & Dong, X. ( 2002; ). Transfer of Bifidobacterium inopinatum and Bifidobacterium denticolens to Scardovia inopinata gen. nov., comb. nov., and Parascardovia denticolens gen. nov., comb. nov., respectively. Int J Syst Evol Microbiol 52, 809–812.[CrossRef]
    [Google Scholar]
  8. Jian, W., Zhu, L. & Dong, X. ( 2001; ). New approach to phylogenetic analysis of the genus Bifidobacterium based on partial HSP60 gene sequences. Int J Syst Evol Microbiol 51, 1633–1638.[CrossRef]
    [Google Scholar]
  9. Jousimies-Somer, H., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. & Finegold, S. M. ( 2002; ). Wadsworth Anaerobic Bacteriology Manual, 6th edn. Belmont, CA. : Star Publishing.
    [Google Scholar]
  10. Kuykendall, L. D., Roy, M. A., O'Neill, J. J. & Devine, T. E. ( 1988; ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradorhizobium japonicum. Int J Syst Bacteriol 38, 358–361.[CrossRef]
    [Google Scholar]
  11. MacKenzie, S. L. ( 1987; ). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70, 151–160.
    [Google Scholar]
  12. Mantzourani, M., Fenlon, M. & Beighton, D. ( 2009a; ). Association between Bifidobacteriaceae and the clinical severity of root caries lesions. Oral Microbiol Immunol 24, 32–37.[CrossRef]
    [Google Scholar]
  13. Mantzourani, M., Gilbert, S. C., Sulong, H. N., Sheehy, E. C., Tank, S., Fenlon, M. & Beighton, D. ( 2009b; ). The isolation of bifidobacteria from occlusal carious lesions in children and adults. Caries Res 43, 308–313.[CrossRef]
    [Google Scholar]
  14. Miller, L. T. ( 1982; ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16, 584–586.
    [Google Scholar]
  15. Munson, M. A., Banerjee, A., Watson, T. F. & Wade, W. G. ( 2004; ). Molecular analysis of the microflora associated with dental caries. J Clin Microbiol 42, 3023–3029.[CrossRef]
    [Google Scholar]
  16. Schleifer, K. H. ( 1985; ). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156.
    [Google Scholar]
  17. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  18. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  19. Tindall, B. J. ( 1990a; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.[CrossRef]
    [Google Scholar]
  20. Tindall, B. J. ( 1990b; ). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef]
    [Google Scholar]
  21. Tindall, B. J., Sikorski, J., Smibert, R. M. & Krieg, N. R. ( 2007; ). Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn, pp. 330–393. Edited by Reddy, C. A., Beveridge, T. J., Breznak, J. A., Marzluf, G., Schmidt, T. M. & Snyder, L. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  22. Wade, W. G., Downes, J., Dymock, D., Hiom, S. J., Weightman, A. J., Dewhirst, F. E., Paster, B. J., Tzellas, N. & Coleman, B. ( 1999; ). The family Coriobacteriaceae: reclassification of Eubacterium exiguum (Poco et al. 1996) and Peptostreptococcus heliotrinreducens (Lanigan 1976) as Slackia exigua gen. nov., comb. nov. and Slackia heliotrinireducens gen. nov., comb. nov., and Eubacterium lentum (Prevot 1938) as Eggerthella lenta gen. nov., comb. nov. Int J Syst Bacteriol 49, 595–600.[CrossRef]
    [Google Scholar]
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Polar lipids of sp. nov. C1A_55 (a) and CCUG 357289 (b). DPG, diphosphatidylglycerol; GL, glycolipid; PGL, phosphoglycolipid; PL, phospholipid.

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