1887

Abstract

A Gram-negative, aerobic, rod-shaped, non-motile strain, BL21, was isolated from landfill soil in Pohang, Korea. Strain BL21 grew optimally at pH 7.0, 30 °C and 0 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain BL21 belonged to the class and was related to the genus . The 16S rRNA gene sequence of strain BL21 was less than 98.30 % similar to those of other species in the genus . DNA–DNA hybridization values with phylogenetically related species of the genus were only 11.7–28.4 %. The major fatty acid components included summed feature 3 (iso-C 2-OH and/or C 7), C, C 7 and C 3-OH. The DNA G+C content was 60.9 mol%. For these reasons, strain BL21 (=KCTC 22399 =JCM 15909) is proposed as a novel species in the genus , with the name sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.019661-0
2010-12-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/12/2715.html?itemId=/content/journal/ijsem/10.1099/ijs.0.019661-0&mimeType=html&fmt=ahah

References

  1. Atlas, R. M. ( 1993; ). Handbook of Microbiological Media. Edited by Parks, L. C.. Boca Raton, FL. : CRC Press.
    [Google Scholar]
  2. Baker, G. C., Smith, J. J. & Cowan, D. A. ( 2003; ). Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 55, 541–555.[CrossRef]
    [Google Scholar]
  3. Buck, J. D. ( 1982; ). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993.
    [Google Scholar]
  4. Choi, J.-H., Kim, M.-S., Roh, S. W. & Bae, J.-W. ( 2010; ). Brevundimonas basaltis sp. nov., isolated from black sand. Int J Syst Evol Microbiol 60, 1488-1492.
    [Google Scholar]
  5. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  7. Gardan, L., Dauga, C., Prior, P., Gillis, M. & Saddler, G. S. ( 2000; ). Acidovorax anthurii sp. nov., a new phytopathogenic bacterium which causes bacterial leaf-spot of anthurium. Int J Syst Evol Microbiol 50, 235–246.[CrossRef]
    [Google Scholar]
  8. Gardan, L., Stead, D. E., Dauga, C. & Gillis, M. ( 2003; ). Acidovorax valerianellae sp. nov., a novel pathogen of lamb's lettuce [Valerianella locusta (L.) Laterr.]. . Int J Syst Evol Microbiol 53, 795–800.[CrossRef]
    [Google Scholar]
  9. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  10. Gonzalez, J. M. & Saiz-Jimenez, C. ( 2002; ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4, 770–773.[CrossRef]
    [Google Scholar]
  11. Heylen, K., Lebbe, L. & De Vos, P. ( 2008; ). Acidovorax caeni sp. nov., a denitrifying species with genetically diverse isolates from activated sludge. Int J Syst Evol Microbiol 58, 73–77.[CrossRef]
    [Google Scholar]
  12. Hirayama, H., Tamaoka, J. & Horikoshi, K. ( 1996; ). Improved immobilization of DNA to microwell plates for DNA-DNA hybridization. Nucleic Acids Res 24, 4098–4099.[CrossRef]
    [Google Scholar]
  13. Kluge, A. G. & Farris, J. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY. : Cold Spring Harbor Laboratory.
    [Google Scholar]
  16. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  17. Schaad, N. W., Postnikova, E., Sechler, A., Claflin, L. E., Vidaver, A. K., Jones, J. B., Agarkova, I., Ignatov, A., Dickstein, E. & Ramundo, B. A. ( 2008; ). Reclassification of subspecies of Acidovorax avenae as A. avenae (Manns 1905) emend., A. cattleyae (Pavarino, 1911) comb. nov., A. citrulli Schaad et al., 1978) comb. nov., and proposal of A. oryzae sp. nov. Syst Appl Microbiol 31, 434–446.[CrossRef]
    [Google Scholar]
  18. Schulze, R., Spring, S., Amann, R., Huber, I., Ludwig, W., Schleifer, K. H. & Kämpfer, P. ( 1999; ). Genotypic diversity of Acidovorax strains isolated from activated sludge and description of Acidovorax defluvii sp. nov. Syst Appl Microbiol 22, 205–214.[CrossRef]
    [Google Scholar]
  19. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  20. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  21. Willems, A., Falsen, E., Pot, B., Jantzen, E., Hoste, B., Vandamme, P., Gillis, M., Kersters, K. & De Ley, J. ( 1990; ). Acidovorax, a new genus for Pseudomonas facilis, Pseudomonas delafieldii, E. Falsen (EF) group 13, EF group 16, and several clinical isolates, with the species Acidovorax facilis comb. nov., Acidovorax delafieldii comb. nov., and Acidovorax temperans sp. nov. Int J Syst Bacteriol 40, 384–398.[CrossRef]
    [Google Scholar]
  22. Willems, A., Goor, M., Thielemans, S., Gillis, M., Kersters, K. & De Ley, J. ( 1992; ). Transfer of several phytopathogenic Pseudomonas species to Acidovorax as Acidovorax avenae subsp. avenae subsp. nov., comb. nov., Acidovorax avenae subsp. citrulli, Acidovorax avenae subsp. cattleyae, and Acidovorax konjaci. Int J Syst Bacteriol 42, 107–119.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.019661-0
Loading
/content/journal/ijsem/10.1099/ijs.0.019661-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 2715 - 2718

Transmission electron micrographs of cells of strain BL21 after growth at 30°C on LB agar. Bar, 0.5 µm.



IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error