1887

Abstract

A thermotolerant, Gram-stain-positive, aerobic, sporangium-forming actinomycete, strain RA45, was isolated from a desert region in Xinjiang Uigur Autonomous Region, north-western China. Comparative analysis of the 16S rRNA gene sequence and phenotypic characterization revealed that strain RA45 belonged phylogenetically to the family of the suborder . Strain RA45 showed more than 5 % 16S rRNA gene sequence divergence from recognized species of genera in the family , forming a distinct lineage within the evolutionary radiation occupied by the genera , , , , and , but distinct from each of them. The affiliation to the family was supported by the presence of suborder- and family-specific 16S rRNA signature nucleotides, a DNA G+C content of 69.9 mol%, the presence of -diaminopimelic acid, ribose, arabinose, glucose and galactose, which are characteristic components of cell-wall chemotype IV of actinomycetes, the presence of menaquinone MK-9(H) as the major respiratory lipoquinone, a lack of mycolic acids and the presence of an -acetylated type of muramic acid. However, strain RA45 differed from known genera of the family in its polar lipid composition: the major phospholipids were phosphatidylethanolamine, phosphatidylinositol mannosides, phosphatidylmethylethanolamine, diphosphatidylglycerol, phospholipids of unknown structure and phospholipids of unknown structure containing glucosamine (phospholipid type IV). Based on its morphological, chemotaxonomic and phylogenetic characteristics, strain RA45 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is RA45 (=CGMCC 4.5579 =JCM 16584).

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2011-03-01
2019-10-14
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