1887

Abstract

Two actinomycetes, designated MN08-A0270 and MN08-A0297, were isolated from soil from the area around Khuvsgul Lake, Khuvsgul province, Mongolia, and subjected to phenotypic and genotypic characterization. They produced well-developed, branched substrate hyphae and, similar to closely related species of the genus , produced zigzag-shaped aerial hyphae by acropetal budding and blastospores. A comparative analysis of 16S rRNA gene sequences indicated that strains MN08-A0270 and MN08-A0297 formed two distinct clades within the genus and were respectively most closely related to NBRC 15681 (97.3 % similarity) and IMSNU 20112 (97.1 %). Chemotaxonomic characteristics, including cell-wall diaminopimelic acid, whole-cell sugars, fatty acid components and major menaquinones, suggested that the two organisms belonged to the genus . Strains MN08-A0270 and MN08-A0297 could be differentiated from each other and from closely related species of the genus by physiological and biochemical characteristics, predominant fatty acids, menaquinones and whole-cell sugar components. Combined with the results of a broad range of phenotypic tests and DNA–DNA hybridization data and phylogenetic analysis, these results support the conclusion that these strains represent two novel species of the genus , for which we propose the names sp. nov. (type strain MN08-A0270  = NBRC 105885  = VTCC D9-25) and sp. nov. (type strain MN08-A0297  = NBRC 105886  = VTCC D9-26).

Funding
This study was supported by the:
  • , Department of Biotechnology, NITE, Japan
  • , Institute of Biology, Mongolian Academy of Sciences, Mongolia
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2011-04-01
2021-03-05
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