1887

Abstract

Two actinomycetes, designated MN08-A0270 and MN08-A0297, were isolated from soil from the area around Khuvsgul Lake, Khuvsgul province, Mongolia, and subjected to phenotypic and genotypic characterization. They produced well-developed, branched substrate hyphae and, similar to closely related species of the genus , produced zigzag-shaped aerial hyphae by acropetal budding and blastospores. A comparative analysis of 16S rRNA gene sequences indicated that strains MN08-A0270 and MN08-A0297 formed two distinct clades within the genus and were respectively most closely related to NBRC 15681 (97.3 % similarity) and IMSNU 20112 (97.1 %). Chemotaxonomic characteristics, including cell-wall diaminopimelic acid, whole-cell sugars, fatty acid components and major menaquinones, suggested that the two organisms belonged to the genus . Strains MN08-A0270 and MN08-A0297 could be differentiated from each other and from closely related species of the genus by physiological and biochemical characteristics, predominant fatty acids, menaquinones and whole-cell sugar components. Combined with the results of a broad range of phenotypic tests and DNA–DNA hybridization data and phylogenetic analysis, these results support the conclusion that these strains represent two novel species of the genus , for which we propose the names sp. nov. (type strain MN08-A0270  = NBRC 105885  = VTCC D9-25) and sp. nov. (type strain MN08-A0297  = NBRC 105886  = VTCC D9-26).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.019562-0
2011-04-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/4/747.html?itemId=/content/journal/ijsem/10.1099/ijs.0.019562-0&mimeType=html&fmt=ahah

References

  1. Adachi J. , Hasegawa M. . ( 1995; ). Improved dating of the human/chimpanzee separation in the mitochondrial DNA tree: heterogeneity among amino acid sites. . J Mol Evol 40:, 622–628. [CrossRef] [PubMed]
    [Google Scholar]
  2. Akimov V. N. , Evtushenko L. I. , Dobritsa S. V. . ( 1989; ). Pseudoamycolata halophobica gen. nov., sp. nov.. Int J Syst Bacteriol 39:, 457–461. [CrossRef]
    [Google Scholar]
  3. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  4. Ara I. , Yamamura H. , Tsetseg B. , Daram D. , Ando K. . ( 2010; a). Luteipulveratus mongoliensis gen. nov., sp. nov., a new actinobacterial taxon in the family Dermacoccaceae . . Int J Syst Evol Microbiol 60:, 574–579. [CrossRef] [PubMed]
    [Google Scholar]
  5. Ara I. , Yamamura H. , Tsetseg B. , Daram D. , Ando K. . ( 2010; b). Actinoplanes toevensis sp. nov. and Actinoplanes tereljensis sp. nov., isolated from Mongolian soil. . Int J Syst Evol Microbiol 60:, 919–927. [CrossRef] [PubMed]
    [Google Scholar]
  6. Chen H.-H. , Qin S. , Li J. , Zhang Y.-Q. , Xu L.-H. , Jiang C.-L. , Kim C.-J. , Li W.-J. . ( 2009; ). Pseudonocardia endophytica sp. nov., isolated from the pharmaceutical plant Lobelia clavata . . Int J Syst Evol Microbiol 59:, 559–563. [CrossRef] [PubMed]
    [Google Scholar]
  7. Duangmal K. , Thamchaipenet A. , Matsumoto A. , Takahashi Y. . ( 2009; ). Pseudonocardia acaciae sp. nov., isolated from roots of Acacia auriculiformis A. Cunn. ex Benth.. Int J Syst Evol Microbiol 59:, 1487–1491. [CrossRef] [PubMed]
    [Google Scholar]
  8. Eck R. V. , Dayhoff M. O. . ( 1966; ). Atlas of Protein Sequences and Structure. Silver Springs, MD:: National Biomedical Research Foundation;.
    [Google Scholar]
  9. Evtushenko L. I. , Akimov V. N. , Dobritsa S. V. , Taptykova S. D. . ( 1989; ). A new species of actinomycete, Amycolata alni . . Int J Syst Bacteriol 39:, 72–77. [CrossRef]
    [Google Scholar]
  10. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  11. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  12. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  13. Gordon R. E. , Mihm J. M. . ( 1957; ). A comparative study of some strains received as nocardiae. . J Bacteriol 73:, 15–27.[PubMed]
    [Google Scholar]
  14. Gordon R. E. , Barnett D. A. , Handerhan J. E. , Pang C. H.-N. . ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  15. Gu Q. , Luo H. , Zheng W. , Liu Z. , Huang Y. . ( 2006; ). Pseudonocardia oroxyli sp. nov., a novel actinomycete isolated from surface-sterilized Oroxylum indicum root. . Int J Syst Evol Microbiol 56:, 2193–2197. [CrossRef] [PubMed]
    [Google Scholar]
  16. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  17. Hasegawa M. , Kishino H. , Yano T. . ( 1985; ). Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. . J Mol Evol 22:, 160–174. [CrossRef] [PubMed]
    [Google Scholar]
  18. Hayakawa M. , Nonomura H. . ( 1987; ). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. . J Ferment Technol 65:, 501–509. [CrossRef]
    [Google Scholar]
  19. Hayakawa M. , Otoguro M. , Takeuchi T. , Yamazaki T. , Iimura Y. . ( 2000; ). Application of a method incorporating differential centrifugation for selective isolation of motile actinomycetes in soil and plant litter. . Antonie van Leeuwenhoek 78:, 171–185. [CrossRef] [PubMed]
    [Google Scholar]
  20. Henssen A. . ( 1957; ). Beiträge zur Morphologie und Systematik der thermophilen Actinomyceten. . Arch Mikrobiol 26:, 373–414 (in German). [CrossRef] [PubMed]
    [Google Scholar]
  21. Henssen A. , Schäfer D. . ( 1971; ). Emended description of the genus Pseudonocardia Henssen and description of a new species Pseudonocardia spinosa Schäfer. . Int J Syst Bacteriol 21:, 29–34. [CrossRef]
    [Google Scholar]
  22. Huang Y. , Wang L. , Lu Z. , Hong L. , Liu Z. , Tan G. Y. A. , Goodfellow M. . ( 2002; ). Proposal to combine the genera Actinobispora and Pseudonocardia in an emended genus Pseudonocardia, and description of Pseudonocardia zijingensis sp. nov.. Int J Syst Evol Microbiol 52:, 977–982. [CrossRef] [PubMed]
    [Google Scholar]
  23. Humble M. W. , King A. , Phillips I. . ( 1977; ). API ZYM: a simple rapid system for the detection of bacterial enzymes. . J Clin Pathol 30:, 275–277. [CrossRef] [PubMed]
    [Google Scholar]
  24. Jiang C. , Xu L. , Yang Y.-R. , Guo G.-Y. , Ma J. , Liu Y. . ( 1991; ). Actinobispora, a new genus of the order Actinomycetales . . Int J Syst Bacteriol 41:, 526–528. [CrossRef]
    [Google Scholar]
  25. Kämpfer P. , Kroppenstedt R. M. . ( 2004; ). Pseudonocardia benzenivorans sp. nov.. Int J Syst Evol Microbiol 54:, 749–751. [CrossRef] [PubMed]
    [Google Scholar]
  26. Kämpfer P. , Kohlweyer U. , Thiemer B. , Andreesen J. R. . ( 2006; ). Pseudonocardia tetrahydrofuranoxydans sp. nov.. Int J Syst Evol Microbiol 56:, 1535–1538. [CrossRef] [PubMed]
    [Google Scholar]
  27. Kloos W. E. , Tornabene T. G. , Schleifer K. H. . ( 1974; ). Isolation and characterization of micrococci from human skin, including two new species: Micrococcus lylae and Micrococcus kristinae . . Int J Syst Bacteriol 24:, 79–101. [CrossRef]
    [Google Scholar]
  28. Kothe H. W. , Vobis G. , Kroppenstedt R. M. , Henssen A. . ( 1989; ). A taxonomic study of mycolateless, wall chemotype IV actinomycetes. . Syst Appl Microbiol 12:, 61–69.[CrossRef]
    [Google Scholar]
  29. Lechevalier M. P. , Lechevalier H. A. . ( 1980; ). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Special Publication 6), pp. 227–291. Arlington, VA:: Society for Industrial Microbiology;.
    [Google Scholar]
  30. Lechevalier M. P. , Prauser H. , Labeda D. P. , Ruan J. S. . ( 1986; ). Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov.. Int J Syst Bacteriol 36:, 29–37. [CrossRef]
    [Google Scholar]
  31. Lee S. D. , Kim E. S. , Hah Y. C. . ( 2000; ). Phylogenetic analysis of the genera Pseudonocardia and Actinobispora based on 16S ribosomal DNA sequences. . FEMS Microbiol Lett 182:, 125–129. [CrossRef] [PubMed]
    [Google Scholar]
  32. Lee S. D. , Kim E. S. , Min K.-L. , Lee W. Y. , Kang S.-O. , Hah Y. C. . ( 2001; ). Pseudonocardia kongjuensis sp. nov., isolated from a gold mine cave. . Int J Syst Evol Microbiol 51:, 1505–1510.[PubMed]
    [Google Scholar]
  33. Lee S. D. , Kim E. S. , Kang S.-O. , Hah Y. C. . ( 2002; ). Pseudonocardia spinosispora sp. nov., isolated from Korean soil. . Int J Syst Evol Microbiol 52:, 1603–1608. [CrossRef] [PubMed]
    [Google Scholar]
  34. Lee S.-B. , Strand S. E. , Stensel H. D. , Herwig R. P. . ( 2004; ). Pseudonocardia chloroethenivorans sp. nov., a chloroethene-degrading actinomycete. . Int J Syst Evol Microbiol 54:, 131–139. [CrossRef] [PubMed]
    [Google Scholar]
  35. Liu Z.-P. , Wu J.-F. , Liu Z. H. , Liu S.-J. . ( 2006; ). Pseudonocardia ammonioxydans sp. nov., isolated from coastal sediment. . Int J Syst Evol Microbiol 56:, 555–558. [CrossRef] [PubMed]
    [Google Scholar]
  36. Mahendra S. , Alvarez-Cohen L. . ( 2005; ). Pseudonocardia dioxanivorans sp. nov., a novel actinomycete that grows on 1,4-dioxane. . Int J Syst Evol Microbiol 55:, 593–598. [CrossRef] [PubMed]
    [Google Scholar]
  37. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  38. McVeigh H. P. , Munro J. , Embley T. M. . ( 1994; ). The phylogenetic position of Pseudoamycolata halophobica (Akimov et al. 1989) and a proposal to reclassify it as Pseudonocardia halophobica . . Int J Syst Bacteriol 44:, 300–302. [CrossRef] [PubMed]
    [Google Scholar]
  39. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  40. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  41. Mori K. , Kim H. , Kakegawa T. , Hanada S. . ( 2003; ). A novel lineage of sulfate-reducing microorganisms: Thermodesulfobiaceae fam. nov., Thermodesulfobium narugense, gen. nov., sp. nov., a new thermophilic isolate from a hot spring. . Extremophiles 7:, 283–290. [CrossRef] [PubMed]
    [Google Scholar]
  42. Nozawa Y. , Sakai N. , Arai K. , Kawasaki Y. , Harada K. . ( 2007; ). Reliable and sensitive analysis of amino acids in the peptidoglycan of actinomycetes using the advanced Marfey’s method. . J Microbiol Methods 70:, 306–311. [CrossRef] [PubMed]
    [Google Scholar]
  43. Park S. W. , Park S. T. , Lee J. E. , Kim Y. M. . ( 2008; ). Pseudonocardia carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete, and an emended description of the genus Pseudonocardia . . Int J Syst Evol Microbiol 58:, 2475–2478. [CrossRef] [PubMed]
    [Google Scholar]
  44. Qin S. , Su Y. Y. , Zhang Y. Q. , Wang H. B. , Jiang C. L. , Xu L. H. , Li W. J. . ( 2008; ). Pseudonocardia ailaonensis sp. nov., isolated from soil in China. . Int J Syst Evol Microbiol 58:, 2086–2089. [CrossRef] [PubMed]
    [Google Scholar]
  45. Qin S. , Zhu W. Y. , Jiang J. H. , Klenk H. P. , Li J. , Zhao G. Z. , Xu L. H. , Li W. J. . ( 2010; ). Pseudonocardia tropica sp. nov., an endophytic actinomycete isolated from the stem of Maytenus austroyunnanensis . . Int J Syst Evol Microbiol 60:, 2524–2528. [CrossRef] [PubMed]
    [Google Scholar]
  46. Rayner R. W. . ( 1970; ). A Mycological Colour Chart. Kew, UK:: Commonwealth Mycological Institute and British Mycological Society;.
    [Google Scholar]
  47. Reichert K. , Lipski A. , Pradella S. , Stackebrandt E. , Altendorf K. . ( 1998; ). Pseudonocardia asaccharolytica sp. nov. and Pseudonocardia sulfidoxydans sp. nov., two new dimethyl disulfide-degrading actinomycetes and emended description of the genus Pseudonocardia . . Int J Syst Bacteriol 48:, 441–449.[PubMed] [CrossRef]
    [Google Scholar]
  48. Rzhetsky A. , Nei M. . ( 1992; ). A simple method for estimating and testing minimum evolution trees. . Mol Biol Evol 9:, 945–967.
    [Google Scholar]
  49. Saito H. , Miura K. . ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72:, 619–629. [CrossRef] [PubMed]
    [Google Scholar]
  50. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  51. Sakiyama Y. , Thao N. K. N. , Vinh H. V. , Giang N. M. , Miyadoh S. , Hop D. V. , Ando K. . ( 2010; ). Pseudonocardia babensis sp. nov., isolated from plant litter. . Int J Syst Evol Microbiol 60:, 2336–2340. [CrossRef] [PubMed]
    [Google Scholar]
  52. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  53. Schäfer J. , Busse H.-J. , Kämpfer P. . ( 2009; ). Pseudonocardia parietis sp. nov., from the indoor environment. . Int J Syst Evol Microbiol 59:, 2449–2452. [CrossRef] [PubMed]
    [Google Scholar]
  54. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  55. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  56. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  57. Takeuchi M. , Nishii T. , Yokota A. . ( 1992; ). Taxonomic significance of arabinose in the family Pseudonocardiaceae. . Actinomycetologica 6:, 79–90. [CrossRef]
    [Google Scholar]
  58. Tamura T. , Hatano K. . ( 2001; ). Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov.. Int J Syst Evol Microbiol 51:, 2119–2125.[PubMed] [CrossRef]
    [Google Scholar]
  59. Tamura T. , Nakagaito Y. , Nishii T. , Hasegawa T. , Stackebrandt E. , Yokota A. . ( 1994; ). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. . Int J Syst Bacteriol 44:, 193–203. [CrossRef] [PubMed]
    [Google Scholar]
  60. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  61. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  62. Warwick S. , Bowen T. , McVeigh H. P. , Embley T. M. . ( 1994; ). A phylogenetic analysis of the family Pseudonocardiaceae and the genera Actinokineospora and Saccharothrix with 16S rRNA sequences and a proposal to combine the genera Amycolata and Pseudonocardia in an emended genus Pseudonocardia . . Int J Syst Bacteriol 44:, 293–299. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.019562-0
Loading
/content/journal/ijsem/10.1099/ijs.0.019562-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error