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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 60, Issue 12

sp. nov., a moderately halophilic Gram-positive bacterium isolated from subsurface saline soil Free

Abstract

A Gram reaction-positive, moderately halophilic bacterium, designated H57B72, was isolated from subsurface saline soil of Qaidam basin in the Qinghai province, China. Cells were rod-shaped, strictly aerobic, spore-forming and motile. The isolate grew optimally at 9 % (w/v) NaCl, pH 7.5 and 30 °C. The cell-wall peptidoglycan of strain H57B72 contained -diaminopimelic acid as the diagnostic diamino acid. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were anteiso-C (59.97 %) and anteiso-C (17.14 %). Phosphatidylglycerol, diphosphatidylglycerol and a glycolipid were found to be the predominant polar lipids. The genomic DNA G+C content of strain H57B72 was 37.1 mol%. 16S rRNA gene sequence analysis showed that strain H57B72 was a member of the genus and was most closely related to DSM 21756 (98.3 % gene sequence similarity). The level of DNA–DNA relatedness between strain H57B72 and DSM 21756 was 8.5 %. Based on the phenotypic, genotypic and phylogenetic data presented, strain H57B72 represents a novel species, for which the name sp. nov. is proposed. The type strain is H57B72 (=DSM 22441 =CGMCC 1.7734).

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Keyword(s): RAPD, random amplification of polymorphic DNA
SGM
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2010-12-01
2024-04-20
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Virgibacillus subterraneus sp. nov., a moderately halophilic Gram-positive bacterium isolated from subsurface saline soil
Int J Syst Evol Microbiol 60, 2763 (2010); https://doi.org/10.1099/ijs.0.019554-0
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