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Abstract

Two Gram-negative, strictly aerobic, non-motile bacteria designated strains KMM 3851 and KMM 3842 were respectively isolated from a marine snail specimen () and from surrounding sediments collected simultaneously from the shore of the Sea of Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains KMM 3851 and KMM 3842 were affiliated with the lineage of the class as a separate phylogenetic line adjacent to the members of the genus . These novel isolates shared 98.5 % 16S rRNA gene sequence similarity and 15 % DNA–DNA relatedness. The major isoprenoid quinone of both strains was Q-10 and polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and aminophospholipids. In addition, strain KMM 3851 contained two unknown lipids, whereas strain KMM 3842 contained diphosphatidylglycerol. Fatty acid analysis revealed C 7 and C as major components and small amounts of C. The DNA G+C contents were 60.4 mol% (KMM 3851) and 58.5 mol% (KMM 3842). Based on distinctive phenotypic characteristics, DNA–DNA hybridization data and phylogenetic distance, strains KMM 3851 and KMM 3842 should be classified as representatives of two novel species in a new genus, gen. nov., with the type species sp. nov. (type strain KMM 3851 =NRIC 0773 =JCM 16493) and a second species sp. nov. (type strain KMM 3842 =NRIC 0772 =JCM 16492).

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2011-01-01
2020-01-27
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Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences available from the GenBank/EMBL/DDBJ databases showing the relationship of strains KMM 3851 and KMM 3842 and related genera of the class . [PDF](47 KB)

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Two-dimensional TLC of polar lipids of KMM 3851 (a) and KMM 3842 (b). DPG, Diphosphatidylglycerol; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; APL, aminophospholipid, L1 and L2, unknown lipids.

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Phase-contrast micrographs displaying cell morphology of strains KMM 3851 (a) and KMM 3842 (b) grown on MA for 3 days at 25 °C. Bars, 5 µm.

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