1887

Abstract

Two Gram-negative, strictly aerobic, non-motile bacteria designated strains KMM 3851 and KMM 3842 were respectively isolated from a marine snail specimen () and from surrounding sediments collected simultaneously from the shore of the Sea of Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains KMM 3851 and KMM 3842 were affiliated with the lineage of the class as a separate phylogenetic line adjacent to the members of the genus . These novel isolates shared 98.5 % 16S rRNA gene sequence similarity and 15 % DNA–DNA relatedness. The major isoprenoid quinone of both strains was Q-10 and polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and aminophospholipids. In addition, strain KMM 3851 contained two unknown lipids, whereas strain KMM 3842 contained diphosphatidylglycerol. Fatty acid analysis revealed C 7 and C as major components and small amounts of C. The DNA G+C contents were 60.4 mol% (KMM 3851) and 58.5 mol% (KMM 3842). Based on distinctive phenotypic characteristics, DNA–DNA hybridization data and phylogenetic distance, strains KMM 3851 and KMM 3842 should be classified as representatives of two novel species in a new genus, gen. nov., with the type species sp. nov. (type strain KMM 3851 =NRIC 0773 =JCM 16493) and a second species sp. nov. (type strain KMM 3842 =NRIC 0772 =JCM 16492).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.019513-0
2011-01-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/1/148.html?itemId=/content/journal/ijsem/10.1099/ijs.0.019513-0&mimeType=html&fmt=ahah

References

  1. Arahal D. R., Macián M. C., Garay E., Pujalte M. J. 2005; Thalassobius mediterraneus gen. nov., sp. nov. and reclassification of Ruegeria gelatinovorans as Thalassobius gelatinovorans comb. nov. Int J Syst Evol Microbiol 55:2371–2376 [CrossRef]
    [Google Scholar]
  2. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917 [CrossRef]
    [Google Scholar]
  3. Boettcher K. J., Geaghan K. K., Maloy A. P., Barber B. J. 2005; Roseovarius crassostreae sp. nov., a member of the Roseobacter clade and the apparent cause of juvenile oyster disease (JOD) in cultured Eastern oysters. Int J Syst Evol Microbiol 55:1531–1537 [CrossRef]
    [Google Scholar]
  4. Buchan A., Gonzalez J. M., Moran M. A. 2005; Overview of the marine Roseobacter lineage. Appl Environ Microbiol 71:5665–5677 [CrossRef]
    [Google Scholar]
  5. Choi D. H., Cho B. C. 2006; Shimia marina gen. nov., sp. nov., a novel bacterium of the Roseobacter clade isolated from biofilm in a coastal fish farm. Int J Syst Evol Microbiol 56:1869–1873 [CrossRef]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  7. Garrity G. M., Bell J. A., Lilburn T. 2005; Order III. Rhodobacterales ord. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn. vol 2Cp–161 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  8. Garrity G. M., Bell J. A., Lilburn T. 2006; Order III. Rhodobacterales ord. nov.In List of New Names and New Combinations Previously Effectively, but not Validly, Published , Validation list no. 107. Int J Syst Evol Microbiol 56:1–6 [CrossRef]
    [Google Scholar]
  9. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  10. Hwang C. Y., Bae G. D., Yih W., Cho B. C. 2009; Marivita cryptomonadis gen. nov., sp. nov. and Marivita litorea sp. nov. of the family Rhodobacteraceae , isolated from marine habitats. Int J Syst Evol Microbiol 59:1568–1575 [CrossRef]
    [Google Scholar]
  11. Kim B. Y., Weon H. Y., Son J. A., Lee C. M., Hong S. B., Jeon Y. A., Koo B. S., Kwon S. W. 2009; Thalassobacter arenae sp. nov., isolated from sea sand in Korea. Int J Syst Evol Microbiol 59:487–490 [CrossRef]
    [Google Scholar]
  12. Lafay B., Ruimy R., Rausch de Traubenberg C., Breittmayer V., Gauthier M. J., Christen R. 1995; Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima . Int J Syst Bacteriol 45:290–296 [CrossRef]
    [Google Scholar]
  13. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  14. Lyman J., Fleming R. H. 1940; Composition of sea water. J Mar Res 3:1183–1184
    [Google Scholar]
  15. Macián M. C., Arahal D. R., Garay E., Ludwig W., Schleifer K. H., Pujalte M. J. 2005; Thalassobacter stenotrophicus gen. nov., sp. nov. a novel marine α -proteobacterium isolated from Mediterranean sea water. Int J Syst Evol Microbiol 55:105–110 [CrossRef]
    [Google Scholar]
  16. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  17. Martens T., Heidorn T., Pukall R., Simon M., Tindall B. J., Brinkhoff T. 2006; Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995)Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter , Ruegeria and Leisingera . Int J Syst Evol Microbiol 56:1293–1304 [CrossRef]
    [Google Scholar]
  18. Owen R. J., Hill L. R., Lapage S. P. 1969; Determination of DNA base composition from melting profiles in dilute buffers. Biopolymers 7:503–516 [CrossRef]
    [Google Scholar]
  19. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448 [CrossRef]
    [Google Scholar]
  20. Pujalte M. J., Macián M. C., Arahal D. R., Garay E. 2005; Thalassobacter stenotrophicus Macián et al. 2005 is a later synonym of Jannaschia cystaugens Adachi et al. 2004, with emended description of the genus Thalassobacter . Int J Syst Evol Microbiol 55:1959–1963 [CrossRef]
    [Google Scholar]
  21. Romanenko L. A., Schumann P., Rohde M., Mikhailov V. V., Stackebrandt E. 2004; Reinekea marinisedimentorum gen. nov., sp. nov. a novel gammaproteobacterium from marine coastal sediments. Int J Syst Evol Microbiol 54:669–673 [CrossRef]
    [Google Scholar]
  22. Romanenko L. A., Uchino M., Falsen E., Frolova G. M., Zhukova N. V., Mikhailov V. V. 2005; Pseudomonas pachastrellae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 55:919–924 [CrossRef]
    [Google Scholar]
  23. Ruiz-Ponte C., Cilia V., Lambert C., Nicolas J. L. 1998; Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus . Int J Syst Bacteriol 48:537–542 [CrossRef]
    [Google Scholar]
  24. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997; Transfer of Bacillus alginolyticus , Bacillus chondroitinus , Bacillus curdlanolyticus , Bacillus glucanolyticus , Bacillus kobensis , and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47:289–298 [CrossRef]
    [Google Scholar]
  25. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology. pp 607–655 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  26. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega 4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  28. Uchino Y., Hirata A., Yokota A., Sugiyama J. 1998; Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen.nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov.,Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44201–210 [CrossRef]
    [Google Scholar]
  29. Uchino Y., Hirata A., Yokota A., Sugiyama J. 1999; Ruegeria algicola comb. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB , List no. 68. Int J Syst Bacteriol 49:1–3 [CrossRef]
    [Google Scholar]
  30. Vaskovsky V. E., Terekhova T. A. 1979; HPTLC of phospholipid mixtures containing phosphatidylglycerol. J High Resol Chromatogr Chromatogr Comm 2:671–672 [CrossRef]
    [Google Scholar]
  31. Wagner-Döbler I., Rheims H., Felske A., Pukall R., Tindall B. J. 2003; Jannaschia helgolandensis gen. nov., sp. nov., a novel abundant member of the marine Roseobacter clade from the North Sea. Int J Syst Evol Microbiol 53:731–738 [CrossRef]
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  33. Yoon J. H., Kang S. J., Oh T. K. 2007a; Donghicola eburneus gen. nov., sp. nov. isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57:73–76 [CrossRef]
    [Google Scholar]
  34. Yoon J. H., Kang S. J., Oh T. K. 2007b; Sulfitobacter marinus sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57:302–305 [CrossRef]
    [Google Scholar]
  35. Yoon J. H., Lee S. Y., Kang S. J., Lee C. H., Oh T. K. 2007c; Pseudoruegeria aquimaris gen. nov., sp. nov. isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57:542–547 [CrossRef]
    [Google Scholar]
  36. Yoon J. H., Kang S. J., Park S., Oh T. K. 2007d; Jannaschia donghaensis sp. nov., isolated from seawater of the East Sea, Korea. Int J Syst Evol Microbiol 57:2132–2136 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.019513-0
Loading
/content/journal/ijsem/10.1099/ijs.0.019513-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error