1887

Abstract

Two halophilic archaea, strains RO1-4 and RO1-64, were isolated from a marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile, and stained Gram-negative. Colonies were red-pigmented. Strains RO1-4 and RO1-64 were able to grow at 25–55 °C (optimum 40–42 °C), at 2.1–5.1 M NaCl (optimum 3.9 M NaCl), at 0.05–0.7 M MgCl (optimum 0.3 M MgCl) and at pH 6.0–8.5 (optimum pH 7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis is 12 % (w/v). On the basis of 16S rRNA gene sequence analysis, strains RO1-4 and RO1-64 were closely related to PR3 (98.0 and 98.2 % similarity, respectively) and BZ256 (97.8 and 97.9 %). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids (S-DGD-1 and DGD-1). The DNA G+C contents of strains RO1-4 and RO1-64 are 64.9 and 62.4 mol%, respectively. The DNA–DNA hybridization value between strains RO1-4 and RO1-64 was 83.0 % and both strains showed low DNA–DNA relatedness with PR3 (42.5 and 50.1 % relatedness, respectively) and BZ256 (37.6 and 42.1 % relatedness). It was concluded that strains RO1-4 and RO1-64 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RO1-4 (=CGMCC 1.7736 =JCM 15770).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.019463-0
2010-11-01
2024-11-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/11/2613.html?itemId=/content/journal/ijsem/10.1099/ijs.0.019463-0&mimeType=html&fmt=ahah

References

  1. Bardavid R. E., Mana L., Oren A. 2007; Haloplanus natans gen. nov., sp. nov., an extremely halophilic, gas-vacuolate archaeon isolated from Dead Sea–Red Sea water mixtures in experimental outdoor ponds. Int J Syst Evol Microbiol 57:780–783 [CrossRef]
    [Google Scholar]
  2. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  3. Cui H.-L., Lin Z.-Y., Dong Y., Zhou P.-J., Liu S.-J. 2007; Halorubrum litoreum sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol 57:2204–2206 [CrossRef]
    [Google Scholar]
  4. Cui H.-L., Zhou P.-J., Oren A., Liu S.-J. 2009; Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium . Extremophiles 13:31–37 [CrossRef]
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  6. Dussault H. P. 1955; An improved technique for staining red halophilic bacteria. J Bacteriol 70:484–485
    [Google Scholar]
  7. Dyall-Smith M. L. 2008; The Halohandbook: protocols for haloarchaeal genetics. http://www.haloarchaea.com/resources/halohandbook
    [Google Scholar]
  8. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  9. Gonzalez C., Gutierrez C., Ramirez C. 1978; Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24:710–715 [CrossRef]
    [Google Scholar]
  10. Gutiérrez C., González C. 1972; Method for simultaneous detection of proteinase and esterase activities in extremely halophilic bacteria. Appl Microbiol 24:516–517
    [Google Scholar]
  11. Gutiérrez M. C., Castillo A. M., Kamekura M., Ventosa A. 2008; Haloterrigena salina sp. nov., an extremely halophilic archaeon isolated from a salt lake. Int J Syst Evol Microbiol 58:2880–2884 [CrossRef]
    [Google Scholar]
  12. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  13. Kates M. 1986 In Techniques of Lipidology, 2nd revised edn, pp. 106–107, 187–188 and 251–254 Amsterdam: Elsevier;
    [Google Scholar]
  14. McDade J. J., Weaver R. H. 1959; Rapid methods for the detection of gelatin hydrolysis. J Bacteriol 77:60–64
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  16. Montalvo-Rodríguez R., Vreeland R. H., Oren A., Kessel M., Betancourt C., López-Garriga J. 1998; Halogeometricum borinquense gen. nov., sp. nov., a novel halophilic archaeon from Puerto Rico. Int J Syst Bacteriol 48:1305–1312 [CrossRef]
    [Google Scholar]
  17. Ng W.-L., Yang C.-F., Halladay J. T., Arora A., DasSarma S. 1995; Protocol 25. Isolation of genomic and plasmid DNAs from Halobacterium halobium . In Archaea: a Laboratory Manual: Halophiles pp 179–180 Edited by DasSarma S., Fleischmann E. M. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Oren A., Ventosa A., Grant W. D. 1997; Proposed minimal standards for description of new taxa in the order Halobacteriales . Int J Syst Bacteriol 47:233–238 [CrossRef]
    [Google Scholar]
  19. Reasoner D. J., Geldreich E. E. 1985; A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49:1–7
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Savage K. N., Krumholz L. R., Oren A., Elshahed M. S. 2008; Halosarcina pallida gen. nov., sp. nov. a halophilic archaeon from a low-salt, sulfide-rich spring. Int J Syst Evol Microbiol 58:856–860 [CrossRef]
    [Google Scholar]
  22. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  23. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  24. Vaskovsky V. E., Kostetsky E. Y. 1968; Modified spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res 9:396
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.019463-0
Loading
/content/journal/ijsem/10.1099/ijs.0.019463-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error