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Volume 60, Issue 11

sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Free

Abstract

Two halophilic archaea, strains RO1-4 and RO1-64, were isolated from a marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile, and stained Gram-negative. Colonies were red-pigmented. Strains RO1-4 and RO1-64 were able to grow at 25–55 °C (optimum 40–42 °C), at 2.1–5.1 M NaCl (optimum 3.9 M NaCl), at 0.05–0.7 M MgCl (optimum 0.3 M MgCl) and at pH 6.0–8.5 (optimum pH 7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis is 12 % (w/v). On the basis of 16S rRNA gene sequence analysis, strains RO1-4 and RO1-64 were closely related to PR3 (98.0 and 98.2 % similarity, respectively) and BZ256 (97.8 and 97.9 %). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids (S-DGD-1 and DGD-1). The DNA G+C contents of strains RO1-4 and RO1-64 are 64.9 and 62.4 mol%, respectively. The DNA–DNA hybridization value between strains RO1-4 and RO1-64 was 83.0 % and both strains showed low DNA–DNA relatedness with PR3 (42.5 and 50.1 % relatedness, respectively) and BZ256 (37.6 and 42.1 % relatedness). It was concluded that strains RO1-4 and RO1-64 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RO1-4 (=CGMCC 1.7736 =JCM 15770).

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Keyword(s): DGD-1, mannosyl glucosyl diether , MP, maximum-parsimony , NJ, neighbour-joining , PG, phosphatidylglycerol , PGP-Me, phosphatidylglycerol phosphate methyl ester and S-DGD-1, sulfated mannosyl glucosyl diether
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2010-11-01
2024-04-19
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Halogeometricum rufum sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Halogeometricum
Int J Syst Evol Microbiol 60, 2613 (2010); https://doi.org/10.1099/ijs.0.019463-0
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