1887

Abstract

Two halophilic archaea, strains RO1-4 and RO1-64, were isolated from a marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile, and stained Gram-negative. Colonies were red-pigmented. Strains RO1-4 and RO1-64 were able to grow at 25–55 °C (optimum 40–42 °C), at 2.1–5.1 M NaCl (optimum 3.9 M NaCl), at 0.05–0.7 M MgCl (optimum 0.3 M MgCl) and at pH 6.0–8.5 (optimum pH 7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis is 12 % (w/v). On the basis of 16S rRNA gene sequence analysis, strains RO1-4 and RO1-64 were closely related to PR3 (98.0 and 98.2 % similarity, respectively) and BZ256 (97.8 and 97.9 %). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids (S-DGD-1 and DGD-1). The DNA G+C contents of strains RO1-4 and RO1-64 are 64.9 and 62.4 mol%, respectively. The DNA–DNA hybridization value between strains RO1-4 and RO1-64 was 83.0 % and both strains showed low DNA–DNA relatedness with PR3 (42.5 and 50.1 % relatedness, respectively) and BZ256 (37.6 and 42.1 % relatedness). It was concluded that strains RO1-4 and RO1-64 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RO1-4 (=CGMCC 1.7736 =JCM 15770).

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2010-11-01
2019-10-20
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Micrographs of cells of strain RO1-4 in liquid medium under optimal conditions. (a) Scanning electron micrograph of strain RO1-4 (bar, 1µm). (b) Phase-contrast micrograph of strain RO1-4 showing pleomorphic forms (bar, 3µm). Strain RO1-64 exhibited similar morphologies.

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Thin-layer chromatograms on Merck silica gel 60 F254 aluminium-backed thin-layer plates of the phospholipids (left) and glycolipids (right) from strain RO1-4 and related species. Lanes: 1 and 7, CGMCC 1.2150 ; 2, JCM 14791 ; 3, BZ256 ; 4, strain RO1-4 ; 5, strain RO1-64; 6, JCM 10706 . PG, phosphatidylglycerol; PGP-Me, phosphatidylglycerol phosphate methyl ester; GL, glycolipid; F, first dimension of TLC; S, second dimension of TLC.

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Phylogenetic tree derived from maximum-parsimony analysis based on 16S rRNA gene sequences showing the relationship between the member of the genus and related genera within the family . Only bootstrap values greater than 70% are shown (1000 replications). Bar, 50 expected changes per 1000nt positions.

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