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Abstract

Novel yellow, obligately methylotrophic and restricted facultatively methylotrophic bacteria, respectively designated strains Ship and Mim, with the ribulose monophosphate pathway of C assimilation are described. Cells were strictly aerobic, Gram-negative, asporogenous, non-motile rods that multiply by binary fission, were mesophilic and neutrophilic and synthesized indole-3-acetic acid and exopolysaccharide. The predominant cellular fatty acids were C and C. The major ubiquinone was Q-8. The predominant phospholipids were phosphatidylethanolamine and phosphatidylglycerol; diphosphatidylglycerol was absent. The two strains lacked -ketoglutarate dehydrogenase and glutamate dehydrogenase. They assimilated ammonium via the glutamate cycle enzymes glutamine synthetase and glutamate synthase. The DNA G+C contents of strains Ship and Mim were 50.7 and 54.5 mol% ( ), respectively. The level of 16S rRNA gene sequence similarity between these strains was very high (99.8 %) but they shared a low level of DNA–DNA relatedness (44 %). Based on 16S rRNA gene sequence analysis and low levels of DNA–DNA relatedness with the type strains of recognized species of the genus (31–36 %), strains Ship and Mim are considered to represent novel species of the genus , for which the names sp. nov. (type strain Ship =DSM 23073 =VKM B-2547 =CCUG 58411) and sp. nov. (type strain Mim =DSM 22949 =VKM B-2548 =CCUG 58412) are proposed.

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2010-11-01
2024-04-19
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