1887

Abstract

Novel yellow, obligately methylotrophic and restricted facultatively methylotrophic bacteria, respectively designated strains Ship and Mim, with the ribulose monophosphate pathway of C assimilation are described. Cells were strictly aerobic, Gram-negative, asporogenous, non-motile rods that multiply by binary fission, were mesophilic and neutrophilic and synthesized indole-3-acetic acid and exopolysaccharide. The predominant cellular fatty acids were C and C. The major ubiquinone was Q-8. The predominant phospholipids were phosphatidylethanolamine and phosphatidylglycerol; diphosphatidylglycerol was absent. The two strains lacked -ketoglutarate dehydrogenase and glutamate dehydrogenase. They assimilated ammonium via the glutamate cycle enzymes glutamine synthetase and glutamate synthase. The DNA G+C contents of strains Ship and Mim were 50.7 and 54.5 mol% ( ), respectively. The level of 16S rRNA gene sequence similarity between these strains was very high (99.8 %) but they shared a low level of DNA–DNA relatedness (44 %). Based on 16S rRNA gene sequence analysis and low levels of DNA–DNA relatedness with the type strains of recognized species of the genus (31–36 %), strains Ship and Mim are considered to represent novel species of the genus , for which the names sp. nov. (type strain Ship =DSM 23073 =VKM B-2547 =CCUG 58411) and sp. nov. (type strain Mim =DSM 22949 =VKM B-2548 =CCUG 58412) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.019455-0
2010-11-01
2021-03-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/11/2623.html?itemId=/content/journal/ijsem/10.1099/ijs.0.019455-0&mimeType=html&fmt=ahah

References

  1. Anthony C., Williams P. 2003; The structure and mechanism of methanol dehydrogenase. Biochim Biophys Acta 1647:18–23 [CrossRef]
    [Google Scholar]
  2. Doronina N. V., Trotsenko Y. A. 1994; Methylophilus leisingerii sp. nov., a new species of restricted facultatively methylotrophic bacteria. Microbiology (English translation of Mikrobiologiia ) 63:529–536
    [Google Scholar]
  3. Doronina N. V., Braus-Strohmeyer S. A., Leisinger T., Trotsenko Y. A. 1995; Isolation and characterization of a new facultatively methylotrophic bacterium: description of Methylorhabdus multivorans gen. nov., sp. nov. . Syst Appl Microbiol 18:92–98 [CrossRef]
    [Google Scholar]
  4. Doronina N. V., Darmaeva T. D., Trotsenko Y. A. 2003; Methylophaga alcalica sp. nov., a novel alkaliphilic and moderately halophilic, obligately methylotrophic bacterium from an East Mongolian saline soda lake. Int J Syst Evol Microbiol 53:223–229 [CrossRef]
    [Google Scholar]
  5. Doronina N. V., Ivanova E. G., Trotsenko Y. A. 2005a; Phylogenetic position and emended description of the genus Methylovorus . Int J Syst Evol Microbiol 55:903–906 [CrossRef]
    [Google Scholar]
  6. Doronina N., Ivanova E., Trotsenko Y., Pshenichnikova A., Kalinina E., Shvets V. 2005b; Methylophilus quaylei sp. nov., a new aerobic obligately methylotrophic bacterium. Syst Appl Microbiol 28:303–309 [CrossRef]
    [Google Scholar]
  7. Felsenstein J. 1989; phylip – phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  8. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  9. Ivanova E., Doronina N., Trotsenko Y. 2007; Hansschlegelia plantiphila gen. nov. sp. nov., a new aerobic restricted facultative methylotrophic bacterium associated with plants. Syst Appl Microbiol 30:444–452 [CrossRef]
    [Google Scholar]
  10. Jenkins O., Byrom D., Jones D. 1987; Methylophilus : a new genus of methanol-utilizing bacteria. Int J Syst Bacteriol 37:446–448 [CrossRef]
    [Google Scholar]
  11. Johnson J. L. 1985; DNA reassociation and RNA hybridization of bacterial nucleic acids. Methods Microbiol 18:33–74
    [Google Scholar]
  12. Kolb S. 2009; Aerobic methanol-oxidizing bacteria in soil. FEMS Microbiol Lett 300:1–10 [CrossRef]
    [Google Scholar]
  13. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  14. Madhaiyan M., Poonguzhali S., Kwon S.-W., Sa T.-M. 2009; Methylophilus rhizosphaerae sp. nov., a restricted facultative methylotroph isolated from rice rhizosphere soil. Int J Syst Evol Microbiol 59:2904–2908 [CrossRef]
    [Google Scholar]
  15. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  16. McDonald I. R., Murrell J. C. 1997; The methanol dehydrogenase structural gene mxaF and its use as a functional gene probe for methanotrophs and methylotrophs. Appl Environ Microbiol 63:3218–3224
    [Google Scholar]
  17. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  18. Urakami T., Komagata K. 1986; Emendation of Methylobacillus Yordy and Weaver 1977, a genus for methanol-utilizing bacteria. Int J Syst Bacteriol 36:502–511 [CrossRef]
    [Google Scholar]
  19. Urakami T., Komagata K. 1987; Characterization of species of marine methylotrophs of the genus Methylophaga . Int J Syst Bacteriol 37:402–406 [CrossRef]
    [Google Scholar]
  20. Van de Peer Y., De Wachter R. 1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
  21. Yordy J. R., Weaver T. Y. 1977; Methylobacillus : a new genus of obligate methylotrophic bacteria. Int J Syst Bacteriol 27:247–255 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.019455-0
Loading
/content/journal/ijsem/10.1099/ijs.0.019455-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error