1887

Abstract

Five Gram-negative, facultatively anaerobic, non-spore-forming, coccoid rod-shaped bacterial isolates were obtained from infant formula and an infant formula production environment and were investigated by use of a polyphasic taxonomic study. Biochemical tests and partial gene sequence analysis of the five isolates revealed that they formed two distinct groups in the family , closely related to several species of the genera and , which indicated a phylogenetic position within the genus or the genus . Multilocus sequence analysis of concatenated partial , , and gene sequences of two of the isolates suggested that they represented two novel species of the genus , phylogenetically related most closely to . The five isolates had general characteristics consistent with those of the genus , and DNA–DNA hybridizations between two representatives and the type strains of their phylogenetically closest relatives based on comparative 16S rRNA gene sequence analysis showed that the isolates represented two novel genospecies. These two genospecies could be differentiated from each other based on fermentation of galacturonate, sorbitol and potassium 5-ketogluconate. They could be differentiated from phylogenetically related species based on their ability to ferment lactose and to utilize -gentiobiose and raffinose, their inability to ferment or utilize -arabitol, and their inability to produce indole. On the basis of the results obtained, the five isolates are considered to represent two novel species of the genus , for which the names sp. nov. (type strain A18/07 =LMG 25382 =DSM 22758) and sp. nov. (type strain 1400/07 =LMG 25383 =DSM 22759) are proposed.

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2010-12-01
2019-10-23
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