1887

Abstract

A Gram-negative, rod-shaped, motile bacterium was isolated from the soil of a ginseng field in Daejeon, South Korea, and characterized in order to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequence analysis revealed that strain DCY34 belonged to the family , and the highest degree of sequence similarity was found with W-50 (97.1 %), Gsoil 250 (97.0 %), S37 (96.9 %), IFO 15033 (96.8 %), RB2256 (96.7 %) and JSS54 (96.7 %). Chemotaxonomic data revealed that strain DCY34 possessed ubiquinone Q-10 as the predominant respiratory lipoquinone, which is common to members of the genus . The predominant fatty acids were C 7 (27.5 %), summed feature 4 (C 7 and/or C iso 2-OH; 18.6 %), C (15.6 %) and summed feature 8 (C 6 and/or unknown 18.864; 15.4 %). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, sphingoglycolipid and an unknown polar lipid. The results of physiological and biochemical tests clearly demonstrated that strain DCY34 represented a separate species and supported its affiliation to the genus . Based on these data, the new isolate represents a novel species, for which the name sp. nov. is proposed. The type strain is DCY34 (=KCTC 22112=JCM 14844).

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2010-10-01
2019-10-14
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Two-dimensional TLC of polar lipids of strain DCY34 visualized with 5% ethanolic molybdatophosphoric acid (for total lipids). PE, Phosphatidylethanolamine; PG, phosphatidylglycerol; DPG, diphosphatidylglycerol; PC, phosphatidylcholine; SLG, sphingoglycolipid; L, unknown polar lipid.

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Cellular fatty acid profiles of strain DCY34 and closely related species. DNA relatedness of DCY34 with closely related type strains of the genus . [ Combined PDF] 42 KB

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