1887

Abstract

A Gram-negative bacterium, designated P2, was isolated from the biofilm developed on the inner surface of an ultrapure cooling water system in a Hungarian power plant and was characterized by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P2 was affiliated with the family ‘’ in the phylum . Its closest relative was CL-GP79 (88.7 % 16S rRNA gene sequence similarity) followed by TW5 (86.5 %), NO-502 (86.4 %), GFA-11 (86.3 %) and DSM 103 (85.4 %). Cells of strain P2 were facultatively anaerobic, non-motile rods. The major fatty acids were C 5 (42.5 %), iso-C 2-OH (17.2 %), iso-C 3-OH (16.1 %) and iso-C (8.5 %). The major menaquinone was MK-7 and the predominant polar lipid was phosphatidylethanolamine. The DNA G+C content was 54.5 mol%. Thus, the phenotypic and genotypic analyses clearly showed that strain P2 is considerably different from members of other genera in the family ‘’. Based on these results, it is concluded that strain P2 represents a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed, with type strain P2 (=DSM 21668 =NCAIM B 02328).

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2010-11-01
2019-10-16
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