RT Journal Article SR Electronic(1) A1 Zhou, Ping Fa A1 Chen, Wei Min A1 Wei, Ge HongYR 2010 T1 Mesorhizobium robiniae sp. nov., isolated from root nodules of Robinia pseudoacacia JF International Journal of Systematic and Evolutionary Microbiology, VO 60 IS 11 SP 2552 OP 2556 DO https://doi.org/10.1099/ijs.0.019356-0 PB Microbiology Society, SN 1466-5034, AB Previously, five rhizobial strains isolated from root nodules of Robinia pseudoacacia were assigned to the same genospecies on the basis of identical 16S rRNA gene sequences and phylogenetic analyses of the nodA, nodC and nifH genes, in which the five isolates formed a well-supported group that excluded other sequences found in public databases. In this study, the 16S rRNA gene sequence similarities between the isolates and Mesorhizobium mediterraneum UPM-Ca36T and Mesorhizobium temperatum SDW018T were 99.5 and 99.6 %, respectively. The five isolates were also different from defined Mesorhizobium species using ERIC fingerprint profiles and they formed a novel Mesorhizobium lineage in phylogenetic analyses of recA and atpD gene sequences. DNA–DNA relatedness values between the representative strain, CCNWYC 115T, and type strains of defined Mesorhizobium species were found to be lower than 47.5 %. These results indicated that the isolates represented a novel genomic species. Therefore, a novel species, Mesorhizobium robiniae sp. nov., is proposed, with type strain CCNWYC 115T (=ACCC 14543T =HAMBI 3082T). Strain CCNWYC 115T can form effective nodules only on its original host., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.019356-0