1887

Abstract

Previously, five rhizobial strains isolated from root nodules of were assigned to the same genospecies on the basis of identical 16S rRNA gene sequences and phylogenetic analyses of the , and genes, in which the five isolates formed a well-supported group that excluded other sequences found in public databases. In this study, the 16S rRNA gene sequence similarities between the isolates and UPM-Ca36 and SDW018 were 99.5 and 99.6 %, respectively. The five isolates were also different from defined species using ERIC fingerprint profiles and they formed a novel lineage in phylogenetic analyses of and gene sequences. DNA–DNA relatedness values between the representative strain, CCNWYC 115, and type strains of defined species were found to be lower than 47.5 %. These results indicated that the isolates represented a novel genomic species. Therefore, a novel species, sp. nov., is proposed, with type strain CCNWYC 115 (=ACCC 14543 =HAMBI 3082). Strain CCNWYC 115 can form effective nodules only on its original host.

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2010-11-01
2024-04-20
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