1887

Abstract

Two lactic acid bacteria, strains 257 and 252, were isolated from traditional heap fermentations of Ghanaian cocoa beans. 16S rRNA gene sequence analysis of these strains allocated them to the genus , showing 99.5 % 16S rRNA gene sequence similarity towards LMG 24286. Whole-cell protein electrophoresis, fluorescent amplified fragment length polymorphism fingerprinting of whole genomes and biochemical tests confirmed their unique taxonomic position. DNA–DNA hybridization experiments towards their nearest phylogenetic neighbour demonstrated that the two strains represent a novel species, for which we propose the name sp. nov., with strain 257 (=LMG 24289 =DSM 21416) as the type strain. Additional sequence analysis using gene sequences proved useful for identification of all species and for the recognition of the novel species.

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2010-09-01
2019-10-21
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vol. , part 9, pp. 1999 - 2005

Neighbour-joining (Fig. S1) and maximum-likelihood (Fig. S2) trees based on 16S rRNA gene sequences showing the phylogenetic relationships of sp. nov. 257 among other strains of the clade.

SDS-PAGE protein profiles and corresponding cluster analysis derived from UPGMA linkage of correlation coefficients for sp. nov. 257 and type strains of all established species.

and strains examined in this study.

[PDF file of Supplementary Figures and Table](100 KB)



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