Proposal of Mingxiaea gen. nov. for the anamorphic basidiomycetous yeast species in the Bulleribasidium clade (Tremellales) based on molecular phylogenetic analysis, with six new combinations and four novel species
The distinction and monophyletic property of the basidiomycetous yeast species in the Bulleribasidium clade of the order Tremellales was resolved by molecular phylogenetic analysis based on the combined sequences of the 18S rRNA gene, internal transcribed spacer (ITS) region including 5.8S rRNA gene and 26S rRNA gene D1/D2 domain. The addition to the clade of new anamorphic species identified among ballistoconidium-forming yeasts isolated from China confirmed and strengthened the separation of this clade from other clades or lineages in the order Tremellales. A new anamorphic genus, Mingxiaea gen. nov. (type species Mingxiaea variabilis comb. nov.) is therefore proposed to accommodate the anamorphic species in the Bulleribasidium clade. Six new combinations are proposed for the described species of this clade which were formerly assigned to the genus Bullera. Four novel species in the new genus were identified among 16 ballistoconidium-forming yeast strains isolated from plant leaves collected in Hainan province, southern China, by D1/D2 and ITS sequence analyses. The novel species are described as Mingxiaea sanyaensis sp. nov. (type strain SY-3.23T =AS 2. 3623T =CBS 11408T), Mingxiaea hainanensis (type strain WZS-8.13T =AS 2.4161T =CBS 11409T), Mingxiaea foliicola (type strain WZS-8.14T =AS 2.3518T =CBS 11407T) and Mingxiaea wuzhishanensis (type strain WZS-29.8T =AS 2.4163T =CBS 11411T).
BaiF.-Y.,
ZhaoJ.-H.,
TakashimaM.,
JiaJ.-H.,
BoekhoutT.,
NakaseT.2002; Reclassification of the Sporobolomyces roseus and Sporidiobolus pararoseus complexes, with the description of Sporobolomyces phaffii sp. nov. Int J Syst Evol Microbiol 52:2309–2314[CrossRef]
BaiF.-Y.,
TakashimaM.,
ZhaoJ.-H.,
JiaJ.-H.,
NakaseT.2003; Bullera anomala sp. nov. and Bullera pseudovariabilis sp. nov., two new ballistoconidium-forming yeast species from Yunnan, China. Antonie van Leeuwenhoek 83:257–263[CrossRef]
BoekhoutT.,
FonsecaA.,
Batenburg-van der VegteW. H.1991; Bulleromyces genus novum (Tremellales), a teleomorph for Bullera alba , and the occurrence of mating in Bullera variabilis
. Antonie van Leeuwenhoek 59:81–93[CrossRef]
FellJ. W.,
BoekhoutT.,
FonsecaA.,
ScorzettiG.,
Statzell-TallmanA.2000; Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351–1371[CrossRef]
FungsinB.,
TakashimaM.,
BaiF.-Y.,
ArtjariyasripongS.,
NakaseT.2003; Bullera panici sp. nov. and Bullera siamensis sp. nov., two new yeasts in the Bullera variabilis cluster isolated in Thailand. Microbiol Cult Collect 19:23–32
FungsinB.,
TakashimaM.,
SugitaT.,
ArtjariyasripongS.,
PotacharoenW.,
TanticharoenM.,
NakaseT.2006; Bullera koratensis sp. nov. and Bullera lagerstroemiae sp. nov., two new ballistoconidium-forming yeast species in the Trichosporonales clade isolated from plant leaves in Thailand. J Gen Appl Microbiol 52:73–81[CrossRef]
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120[CrossRef]
NakaseT.,
SuzukiM.1987; Bullera variabilis , a new species of yeast with uniquely-shaped ballistospores isolated from various plant materials. J Gen Appl Microbiol 33:343–354[CrossRef]
NakaseT.,
TakashimaM.1993; A simple procedure for the high frequency isolation of new taxa of ballistosporous yeasts living on the surfaces of plants. RIKEN Review 3:33–34
NakaseT.,
TsuzukiS.,
TakashimaM.2002; Bullera taiwanensis sp. nov. and Bullera formosensis sp. nov., two new ballistoconidium-forming yeasts isolated from plant leaves in Taiwan. J Gen Appl Microbiol 48:345–355[CrossRef]
NakaseT.,
TsuzukiS.,
LeeF. L.,
JindamorakotS.,
Jan-ngamH.,
PotacharoenW.,
TanticharoenM.,
KudoT.,
TakashimaM.2004; Bullera begoniae sp. nov. and Bullera setariae sp. nov.,two new species of ballistoconidium-forming yeasts in the Bullera variabilis (Bulleribasidium) cluster isolated from plants in Taiwan. Mycoscience 45:287–294[CrossRef]
SampaioJ. P.,
WeißM.,
GadanhoM.,
BauerR.2002 New taxa in the Tremellales: Bulleribasidium oberjochense gen.et sp. nov., Papiliotrema bandonii gen. et sp.nov. and Fibulobasidium murrhardtense sp. nov. Mycologia 94873–887[CrossRef]
ScorzettiG.,
FellJ. W.,
FonsecaA.,
Statzell-TallmanA.2002; Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res 2:495–517[CrossRef]
TakashimaM.,
NakaseT.1999; Molecular phylogeny of the genus Cryptococcus and related species based on the sequences of 18S rDNA and internal transcribed spacer regions. Microbiol Cult Collect 15:35–47
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
WangQ.-M.,
BaiF.-Y.2008; Molecular phylogeny of basidiomycetous yeasts in the Cryptococcus luteolus lineage (Tremellales) based on nuclear rRNA and mitochondrial cytochrome b gene sequence analyses: proposal of Derxomyces gen.nov. and Hannaella gen. nov.,and description of eight novel Derxomyces species. FEMS Yeast Res 8:799–814[CrossRef]
YamadaY.,
KondoK.1973; Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus and the yeast-like genera Sporobolomyces and Rhodosporidium
. J Gen Appl Microbiol 19:59–77[CrossRef]
YarrowD.1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn. pp 77–100 Edited by
KurtzmanC. P.,
FellJ. W.
Amsterdam: Elsevier;
Proposal of Mingxiaea gen. nov. for the anamorphic basidiomycetous yeast species in the Bulleribasidium clade (Tremellales) based on molecular phylogenetic analysis, with six new combinations and four novel species