1887

Abstract

The distinction and monophyletic property of the basidiomycetous yeast species in the clade of the order Tremellales was resolved by molecular phylogenetic analysis based on the combined sequences of the 18S rRNA gene, internal transcribed spacer (ITS) region including 5.8S rRNA gene and 26S rRNA gene D1/D2 domain. The addition to the clade of new anamorphic species identified among ballistoconidium-forming yeasts isolated from China confirmed and strengthened the separation of this clade from other clades or lineages in the order Tremellales. A new anamorphic genus, gen. nov. (type species comb. nov.) is therefore proposed to accommodate the anamorphic species in the clade. Six new combinations are proposed for the described species of this clade which were formerly assigned to the genus . Four novel species in the new genus were identified among 16 ballistoconidium-forming yeast strains isolated from plant leaves collected in Hainan province, southern China, by D1/D2 and ITS sequence analyses. The novel species are described as sp. nov. (type strain SY-3.23 =AS 2. 3623 =CBS 11408), (type strain WZS-8.13 =AS 2.4161 =CBS 11409), (type strain WZS-8.14 =AS 2.3518 =CBS 11407) and (type strain WZS-29.8 =AS 2.4163 =CBS 11411).

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2011-01-01
2019-10-16
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vol. , part 1, pp. 210 - 219

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Phylogenetic tree reconstructed from neighbour-joining analysis of the combined sequences of the ITS region (including 5.8S rRNA gene) and 26S rRNA gene D1/D2 domain depicting the relationships of almost all recognized yeast species in the order Tremellales. Bar, 0.02 substitutions per nucleotide position.

Consensus tree reconstructed from maximum-parsimony analysis of the combined sequences of the 18S rRNA gene, ITS region (including 5.8S rRNA gene) and 26S rRNA gene D1/D2 domain depicting the relationships of the clades or lineages in the Tremellomycetes. All positions containing gaps were eliminated and there were a total of 2322 positions in the final dataset, out of which 592 were parsimony informative. Bootstrap percentages over 50 % from 1000 bootstrap replicates are shown. Sequences are from type strains of the species compared, either determined in this study (in bold) or retrieved from GenBank under the accession numbers indicated. Bar, 50 substitutions.



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