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Abstract

A Gram-negative, facultatively aerobic, oxidase- and catalase-positive, rod-shaped bacterium, designated strain E407-8, was isolated from a sediment sample from the South China Sea. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain E407-8 was affiliated with the genus , sharing the highest sequence similarity with KMM 1406 (97.5 %) and SW-353 (97.4 %) and showing less than 97 % sequence similarity to the type strains of other recognized species. Levels of DNA–DNA relatedness of strain E407-8 to DSM 17616 and JCM 14331 were 25.2 % (25.3 % in the duplicate measurement) and 9.4 % (6.5 %), respectively. The bacterium could grow at 10–48 °C (optimum 37 °C) and in the presence of 0–8 % (w/v) NaCl (optimum 0.5–2.5 %). The major cellular fatty acids of strain E407-8 were summed feature 3 (Cω7 and/or iso-C 2-OH), Cω8, C and Cω7. The predominant respiratory quinone was ubiquinone Q-8. The DNA G+C content was 51.0 mol%. Based on the results of our polyphasic taxonomic study, strain E407-8 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is E407-8 ( = CCTCC AB 209089  = KACC 14030). An emended description of the genus Brettar 2002 emend. Merchant 2007 is also proposed.

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2011-05-01
2019-12-06
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References

  1. Brettar I. , Christen R. , Höfle M. G. . ( 2002; ). Rheinheimera baltica gen. nov., sp. nov., a blue-coloured bacterium isolated from the central Baltic Sea. . Int J Syst Evol Microbiol 52:, 1851–1857. [CrossRef] [PubMed]
    [Google Scholar]
  2. Brettar I. , Christen R. , Höfle M. G. . ( 2006; ). Rheinheimera perlucida sp. nov., a marine bacterium of the Gammaproteobacteria isolated from surface water of the central Baltic Sea. . Int J Syst Evol Microbiol 56:, 2177–2183. [CrossRef] [PubMed]
    [Google Scholar]
  3. Chen L. P. , Xu H. Y. , Fu S. Z. , Fan H. X. , Liu Y. H. , Liu S. J. , Liu Z. P. . ( 2009; ). Glaciecola lipolytica sp. nov., isolated from seawater near Tianjin city, China. . Int J Syst Evol Microbiol 59:, 73–76. [CrossRef] [PubMed]
    [Google Scholar]
  4. Chiu H. H. , Shieh W. Y. , Lin S. Y. , Tseng C. M. , Chiang P. W. , Wagner-Döbler I. . ( 2007; ). Alteromonas tagae sp. nov. and Alteromonas simiduii sp. nov., mercury-resistant bacteria isolated from a Taiwanese estuary. . Int J Syst Evol Microbiol 57:, 1209–1216. [CrossRef] [PubMed]
    [Google Scholar]
  5. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  7. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  8. Halpern M. , Senderovich Y. , Snir S. . ( 2007; ). Rheinheimera chironomi sp. nov., isolated from a chironomid (Diptera; Chironomidae) egg mass. . Int J Syst Evol Microbiol 57:, 1872–1875. [CrossRef] [PubMed]
    [Google Scholar]
  9. Hedlund B. P. , Gosink J. J. , Staley J. T. . ( 1997; ). Verrucomicrobia div. nov., a new division of the bacteria containing three new species of Prosthecobacter . . Antonie van Leeuwenhoek 72:, 29–38. [CrossRef] [PubMed]
    [Google Scholar]
  10. Huß V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192.[CrossRef]
    [Google Scholar]
  11. Jean W. D. , Shieh W. Y. , Chiu H. H. . ( 2006; ). Pseudidiomarina taiwanensis gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the family Idiomarinaceae . . Int J Syst Evol Microbiol 56:, 899–905. [CrossRef] [PubMed]
    [Google Scholar]
  12. Jukes T. H. , Cantor C. R. . ( 1969; ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N. . . New York:: Academic Press;.
    [Google Scholar]
  13. Lane D. J. . ( 1991; ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. , Goodfellow M. . . Chichester:: Wiley;.
    [Google Scholar]
  14. Merchant M. M. , Welsh A. K. , McLean R. J. C. . ( 2007; ). Rheinheimera texasensis sp. nov., a halointolerant freshwater oligotroph. . Int J Syst Evol Microbiol 57:, 2376–2380. [CrossRef] [PubMed]
    [Google Scholar]
  15. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  16. Murray R. G. E. , Doetsch R. N. , Robinow C. F. . ( 1994; ). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  17. Romanenko L. A. , Uchino M. , Falsen E. , Zhukova N. V. , Mikhailov V. V. , Uchimura T. . ( 2003; ). Rheinheimera pacifica sp. nov., a novel halotolerant bacterium isolated from deep sea water of the Pacific. . Int J Syst Evol Microbiol 53:, 1973–1977. [CrossRef] [PubMed]
    [Google Scholar]
  18. Ryu S. H. , Chung B. S. , Park M. , Lee S. S. , Lee S. S. , Jeon C. O. . ( 2008; ). Rheinheimera soli sp. nov., a gammaproteobacterium isolated from soil in Korea. . Int J Syst Evol Microbiol 58:, 2271–2274. [CrossRef] [PubMed]
    [Google Scholar]
  19. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  20. Shieh W. Y. , Lin Y. T. , Jean W. D. . ( 2004; ). Pseudovibrio denitrificans gen. nov., sp. nov., a marine, facultatively anaerobic, fermentative bacterium capable of denitrification. . Int J Syst Evol Microbiol 54:, 2307–2312. [CrossRef] [PubMed]
    [Google Scholar]
  21. Shieh W. Y. , Liu T. Y. , Lin S. Y. , Jean W. D. , Chen J. S. . ( 2008; ). Simiduia agarivorans gen. nov., sp. nov., a marine, agarolytic bacterium isolated from shallow coastal water from Keelung, Taiwan. . Int J Syst Evol Microbiol 58:, 895–900. [CrossRef] [PubMed]
    [Google Scholar]
  22. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  23. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  24. Stackebrandt E. , Frederiksen W. , Garrity G. M. , Grimont P. A. D. , Kämpfer P. , Maiden M. C. J. , Nesme X. , Rosselló-Mora R. , Swings J. et al. ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52:, 1043–1047. [CrossRef] [PubMed]
    [Google Scholar]
  25. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  26. Van Trappen S. , Tan T. L. , Yang J. , Mergaert J. , Swings J. . ( 2004; a). Alteromonas stellipolaris sp. nov., a novel, budding, prosthecate bacterium from Antarctic seas, and emended description of the genus Alteromonas . . Int J Syst Evol Microbiol 54:, 1157–1163. [CrossRef] [PubMed]
    [Google Scholar]
  27. Van Trappen S. , Tan T. L. , Yang J. , Mergaert J. , Swings J. . ( 2004; b). Glaciecola polaris sp. nov., a novel budding and prosthecate bacterium from the Arctic Ocean, and emended description of the genus Glaciecola . . Int J Syst Evol Microbiol 54:, 1765–1771. [CrossRef] [PubMed]
    [Google Scholar]
  28. Vandecandelaere I. , Nercessian O. , Segaert E. , Achouak W. , Mollica A. , Faimali M. , De Vos P. , Vandamme P. . ( 2008; ). Alteromonas genovensis sp. nov., isolated from a marine electroactive biofilm and emended description of Alteromonas macleodii Baumann et al. 1972 (Approved Lists 1980). . Int J Syst Evol Microbiol 58:, 2589–2596. [CrossRef] [PubMed]
    [Google Scholar]
  29. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. ( 1987; ). Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  30. Yoon J.-H. , Bae S. E. , Kang S.-J. , Oh T.-K. . ( 2007; ). Rheinheimera aquimaris sp. nov., isolated from seawater of the East Sea in Korea. . Int J Syst Evol Microbiol 57:, 1386–1390. [CrossRef] [PubMed]
    [Google Scholar]
  31. Young K. D. . ( 2006; ). The selective value of bacterial shape. . Microbiol Mol Biol Rev 70:, 660–703. [CrossRef] [PubMed]
    [Google Scholar]
  32. Zhang X. X. , Sun L. , Qiu F. B. , McLean R. J. C. , Jiang R. B. , Song W. . ( 2008; ). Rheinheimera tangshanensis sp. nov., a rice root-associated bacterium. . Int J Syst Evol Microbiol 58:, 2420–2424. [CrossRef] [PubMed]
    [Google Scholar]
  33. Zhou M. Y. , Chen X. L. , Zhao H. L. , Dang H. Y. , Luan X. W. , Zhang X. Y. , He H. L. , Zhou B. C. , Zhang Y. Z. . ( 2009; ). Diversity of both the cultivable protease-producing bacteria and their extracellular proteases in the sediments of the South China sea. . Microb Ecol 58:, 582–590. [CrossRef] [PubMed]
    [Google Scholar]
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vol. , part 5, pp. 1016 - 1022

Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences showing the phylogenetic positions of strain E407-8 , members of other species and representatives of some other related genera. [PDF](41 KB)



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