1887

Abstract

Two strains of a novel bacterium were isolated independently of each other, from different depths in the Mediterranean Sea, within a time period of 7 months, using two different isolation approaches that were focused on different objectives. Both strains, designated ISO1 and ISO4, were halophilic, Gram-negative, strictly aerobic, straight rods that were oxidase- and catalase-positive. Both strains produced mucoid colonies in some defined minimal media and were able to grow with organic acids and some alkanes; they were also able to accumulate intracellular poly-β-hydroxybutyrate granules. The G+C content of the DNA of strain ISO4 was 66 mol%. Comparative analysis of 16S rRNA gene sequences showed that the closest described species to the novel strains were and , both of the γ-. Both of these recognized species were originally isolated from North Sea waters and are able to degrade aliphatic compounds, a property shared with strains ISO1 and ISO4. However, strains ISO1 and ISO4 were different from and , not only in their 16S rDNA sequences but also in the motility of their cells (by polar flagella) and by the presence of C in their cellular fatty acids, among other differential features. On the basis of biochemical and molecular data, it is suggested that strains ISO1 and ISO4 be recognized as a novel species of the genus , for which the name (ISO4 =DSM 13974 =CECT 5388) is proposed. On the basis of its high phenotypic similarity and close phylogenetic relatedness to , it is also proposed that (DSM 12178) be reclassified as in the genus , and that the description of the genus be emended.

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2003-01-01
2019-12-15
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