1887

Abstract

Two bacterial strains, II-B4 and II-D5, isolated from the meso-eutrophic freshwater Římov reservoir (Czech Republic), were characterized phenotypically, phylogenetically and chemotaxonomically. Both strains were chemo-organotrophic, facultatively anaerobic, non-motile rods, with identical DNA G+C contents of 59.9 mol%. Their major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and their major fatty acids were C 7/C 6, C, C 7/C 6 and C. Both strains contained Q-8 as the only respiratory quinone component. The 16S rRNA gene sequences of the two strains possessed 99.1 % similarity; however, the level of DNA–DNA reassociation was only 26.7 %. The strains can also be discriminated from each other by several chemotaxonomic and biochemical traits. Phylogenetic analysis of the 16S rRNA gene sequences revealed the affiliation of both strains with the genus within the family . The two investigated strains represent the first isolated members of a narrow phylogenetic cluster (the so-called R-BT065 cluster) formed by a large number of environmental sequences and abundant populations detected in the pelagic zones of various freshwater habitats. We propose to place the two strains in separate novel species within the genus , sp. nov., with the type strain II-D5 (=DSM 21594 =CIP 109844), and sp. nov., with the type strain II-B4 (=DSM 21592 =CIP 109845). The description of the genus is emended accordingly.

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2010-12-01
2019-10-23
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Rímov Reservoir is located near Ceské Budejovice, Czech Republic, and is a source of drinking water. Note that not all diacritical marks in the original table can be represented consistently in HTML; a PDF versionof this table is also available.

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Electron microscopy images illustrating the cell morphology and size of strains II-B4 (a) and II-D5 (b). Cells of a liquid culture were concentrated by centrifugation and fixed with 2.5 % glutaraldehyde, post-fixed with OsO and embedded with Spurr resin. Ultrathin sections were counterstained with uranyl acetate and lead citrate. Images was obtained with a JEOL JEM-1010 transmission electron microscope. Bars, 200 nm.

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Two-dimensional TLC of polar lipids of strains II-B4 (a) and II-D5 (b). DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; PL1, PL2, PL3, unknown phospholipids; PN, unknown aminophospholipid. Polar lipid analysis was carried out by the Identification Service of the DSMZ and Dr Brian J. Tindall (DSMZ, Braunschweig, Germany).

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Neighbour-joining tree calculated with almost-complete 16S rRNA gene sequences of cultivated and uncultivated relatives of strains II-B4 and II-D5 . PDF(40 KB)

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