1887

Abstract

Two strains (JA480 and JA481) of phototrophic alphaproteobacteria were isolated from sediment samples collected from brackish water near Nagapattinam, India. They were Gram-negative, vibrioid cells containing bacteriochlorophyll and rhodopin as major photosynthetic pigments. Most cells of both strains were non-flagellated; 1 % of cells showed two monopolar flagella. Cells showed positive phototaxis. Both strains showed chimeric intracellular photosynthetic membranes, where both lamellar stacks and vesicles were present together in a single cell. The major fatty acids were Cω7 and C in both strains. The genomic G+C content was 67.2–68.8 mol% and the two strains were closely related (mean DNA–DNA hybridization >85 %). 16S rRNA gene sequence comparisons indicated that the isolates represent members of the within the class . According to the sequence comparison data, strain JA480 is positioned distinctly outside the group formed by the phototrophic genera , , , , and , with only 80–92 % 16S rRNA gene sequence similarity. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of this isolate as a representative of a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is JA480 ( = KCTC 5825  = NBRC 106163  = DSM 23193).

Funding
This study was supported by the:
  • Department of Science and Technology and Department of Biotechnology, Government of India
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2011-04-01
2024-12-04
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410[PubMed] [CrossRef]
    [Google Scholar]
  2. Anil Kumar P., Srinivas T. N. R., Takaichi S., Maoka T., Sasikala Ch., Ramana Ch. V. 2009; Phaeospirillum chandramohanii sp. nov., a phototrophic alphaproteobacterium with carotenoid glycosides. Int J Syst Evol Microbiol 59:2089–2093 [View Article][PubMed]
    [Google Scholar]
  3. Biebl H., Pfennig N. 1981; Isolation of members of the family Rhodospirillaceae . In The Prokaryotes: A Handbook on Habitats, Isolation, and Identification of Bacteria vol. 1 pp. 267–273 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. New York: Springer;
    [Google Scholar]
  4. Britton, G., Liaaen-Jensen, S. & Pfander, H. (editors) (2004). Carotenoids with a C40 skeleton. (a) Hydrocarbons. In Carotenoids Handbook, Main List, no. 31. Basel: Birkhauser.
  5. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [View Article][PubMed]
    [Google Scholar]
  6. Chèneby D., Philippot L., Hartmann A., Hénault C., Germon J. C. 2000; 16S rDNA analysis for characterization of denitrifying bacteria isolated from three agricultural soils. FEMS Microbiol Ecol 34:121–128[PubMed] [CrossRef]
    [Google Scholar]
  7. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  8. Glaeser J., Overmann J. 1999; Selective enrichment and characterization of Roseospirillum parvum, gen. nov. and sp. nov., a new purple nonsulfur bacterium with unusual light absorption properties. Arch Microbiol 171:405–416 [View Article][PubMed]
    [Google Scholar]
  9. Guyoneaud R., Mouné S., Eatock C., Bothorel V., Hirschler-Réa A., Willison J., Duran R., Liesack W., Herbert R. et al. 2002; Characterization of three spiral-shaped purple nonsulfur bacteria isolated from coastal lagoon sediments, saline sulfur springs, and microbial mats: emended description of the genus Roseospira and description of Roseospira marina sp. nov., Roseospira navarrensis sp. nov., and Roseospira thiosulfatophila sp. nov.. Arch Microbiol 178:315–324 [View Article][PubMed]
    [Google Scholar]
  10. Imhoff J. F. 2005a; Genus I. Rhodospirillum Molisch 1907, 24AL emend. Imhoff, Petri and Suling 1998, 796. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2C pp. 1–6 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer; [View Article]
    [Google Scholar]
  11. Imhoff J. F. 2005b; Genus V. Phaeospirillum Imhoff, Petri and Suling 1998, 796VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2C pp. 32–33 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer; [View Article]
    [Google Scholar]
  12. Imhoff J. F., Pfennig N. 2001; Thioflavicoccus mobilis gen. nov., sp. nov., a novel purple sulfur bacterium with bacteriochlorophyll b . Int J Syst Evol Microbiol 51:105–110[PubMed]
    [Google Scholar]
  13. Imhoff J. F., Petri R., Süling J. 1998a; Reclassification of species of the spiral-shaped phototrophic purple non-sulfur bacteria of the α-Proteobacteria: description of the new genera Phaeospirillum gen. nov., Rhodovibrio gen. nov., Rhodothalassium gen. nov. and Phaeospirillum gen. nov. as well as transfer of Rhodospirillum fulvum to Phaeospirillum fulvum comb. nov., of Rhodospirillum molischianum to Phaeospirillum molischianum comb. nov., of Rhodospirillum salinarum to Rhodovibrio salinarum comb. nov., of Rhodospirillum sodomense to Rhodovibrio sodomensis comb. nov., of Rhodospirillum salexigens to Rhodothalassium salexigens comb. nov. and of Rhodospirillum mediosalinum to Roseospira mediosalina comb. nov.. Int J Syst Bacteriol 48:793–798 [View Article][PubMed]
    [Google Scholar]
  14. Imhoff J. F., Süling J., Petri R. 1998b; Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium . Int J Syst Bacteriol 48:1129–1143 [View Article][PubMed]
    [Google Scholar]
  15. Kalyan Chakravarthy S., Srinivas T. N. R., Anil Kumar P., Sasikala Ch., Ramana Ch. V. 2007; Roseospira visakhapatnamensis sp. nov. and Roseospira goensis sp. nov.. Int J Syst Evol Microbiol 57:2453–2457 [View Article][PubMed]
    [Google Scholar]
  16. Kawasaki H., Hoshino Y., Kuraishi Y., Yamasato K. 1992; Rhodocista centenaria gen. nov., sp. nov., a cyst-forming anoxygenic photosynthetic bacterium and its phylogenetic position in the Proteobacteria alpha group. J Gen Appl Microbiol 38:541–551 [View Article]
    [Google Scholar]
  17. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82:6955–6959 [View Article][PubMed]
    [Google Scholar]
  18. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  20. Pfennig N., Lünsdorf H., Süling J., Imhoff J. F. 1997; Rhodospira trueperi gen. nov., spec. nov., a new phototrophic Proteobacterium of the alpha group. Arch Microbiol 168:39–45 [View Article][PubMed]
    [Google Scholar]
  21. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 74:5463–5467 [View Article][PubMed]
    [Google Scholar]
  22. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, Technical Note 101. Newark, DE: MIDI, Inc.
  23. Seldin L., Dubnau D. 1985; Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35:151–154 [View Article]
    [Google Scholar]
  24. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  25. Trüper H. G., Pfennig N. 1981; Characterization and identification of the anoxygenic phototrophic bacteria. In The Prokaryotes: a Handbook on Habitats, Isolation and Identification of Bacteria vol. 1 pp. 298–312 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. New York: Springer;
    [Google Scholar]
  26. Zhang D., Yang H., Zhang W., Huang Z., Liu S. J. 2003; Rhodocista pekingensis sp. nov., a cyst-forming phototrophic bacterium from a municipal wastewater treatment plant. Int J Syst Evol Microbiol 53:1111–1114 [View Article][PubMed]
    [Google Scholar]
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