1887

Abstract

Two strains (JA480 and JA481) of phototrophic alphaproteobacteria were isolated from sediment samples collected from brackish water near Nagapattinam, India. They were Gram-negative, vibrioid cells containing bacteriochlorophyll and rhodopin as major photosynthetic pigments. Most cells of both strains were non-flagellated; 1 % of cells showed two monopolar flagella. Cells showed positive phototaxis. Both strains showed chimeric intracellular photosynthetic membranes, where both lamellar stacks and vesicles were present together in a single cell. The major fatty acids were Cω7 and C in both strains. The genomic G+C content was 67.2–68.8 mol% and the two strains were closely related (mean DNA–DNA hybridization >85 %). 16S rRNA gene sequence comparisons indicated that the isolates represent members of the within the class . According to the sequence comparison data, strain JA480 is positioned distinctly outside the group formed by the phototrophic genera , , , , and , with only 80–92 % 16S rRNA gene sequence similarity. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of this isolate as a representative of a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is JA480 ( = KCTC 5825  = NBRC 106163  = DSM 23193).

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2011-04-01
2019-10-24
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(a) Phase-contrast micrograph of cells of strain JA480 , Bar, 5 µm. (b) Electron micrograph of a negatively stained cell of strain JA480 showing the absence of flagella, as seen in the majority of cells. Bar, 500 nm. (c) Electron micrograph of negatively stained cells of strain JA480 showing monopolar biflagella. Bar, 540 nm.

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(a) Motility assay tubes, with soft agar (0.5%), stabbed with cultures of JA145 and strain JA480 . (b) Demonstration of phototaxis in strain JA480 . (i) Tube incubated in the dark, showing no growth (foil removed for photography); (ii) tube incubated to allow illumination only from the bottom, showing phototaxis. An aliquot (100 µl) of the culture was added as inoculum to the top of the soft agar medium and incubated for 5 days.

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[PDF file of Supplementary Figs S3 and S4](139 KB)

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