1887

Abstract

A Gram-positive, endospore-forming, rod-shaped bacterium, strain PD-A10, was isolated from salted crab () in Thailand and subjected to a taxonomic study. Phenotypic and chemotaxonomic characteristics, including phylogenetic analyses, showed that the novel strain was a member of the genus . The novel strain grew in medium with 0–14 % (w/v) NaCl, at 4–55 °C and at pH 4.5–9. The predominant quinone was a menaquinone with seven isoprene units (MK-7). The major fatty acids were anteiso-C and anteiso-C. Polar lipid analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysylphosphatidylglycerol, glycolipid and unknown lipids. The DNA G+C content was 41.4 mol%. The 16S rRNA gene sequence similarities between strain PD-A10 and NBRC 15535, DSM 10, DSM 11031 and IFO 15718 were 99.5, 99.4, 99.4 and 99.2 %, respectively. Strain PD-A10 showed a low degree similarity of rep-PCR fingerprints and low DNA–DNA relatedness with the above-mentioned species. On the basis of the data gathered in this study, strain PD-A10 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PD-A10 (=BCC 22614=KCTC 13613).

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2010-10-01
2019-12-06
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vol. , part 10, pp. 2364 - 2370

Two-dimensional thin layer chromatography of polar lipids of strain PD-A10 . Chloroform/methanol/water (65:25:4) was used in the first direction, followed by chloroform/acetic acid/methanol/water (40:7.5:6:2) in the second direction. DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; LPG, lysylphosphatidylglycerol; GL, glycolipid; APL, unknown aminophospholipid; AL1-AL4, unknown aminolipid; L1-L2, unknown lipid.



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