%0 Journal Article %A Lavrinenko, Ksenia %A Chernousova, Elena %A Gridneva, Elena %A Dubinina, Galina %A Akimov, Vladimir %A Kuever, Jan %A Lysenko, Anatoly %A Grabovich, Margarita %T Azospirillum thiophilum sp. nov., a diazotrophic bacterium isolated from a sulfide spring %D 2010 %J International Journal of Systematic and Evolutionary Microbiology, %V 60 %N 12 %P 2832-2837 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.018853-0 %I Microbiology Society, %X A novel nitrogen-fixing strain, designated BV-ST, was isolated from a sulfur bacterial mat collected from a sulfide spring of the Stavropol Krai, North Caucasus, Russia. Strain BV-ST grew optimally at pH 7.5 and 37 °C. According to the results of phylogenetic analysis, strain BV-ST belonged to the genus Azospirillum within the family Rhodospirillaceae of the class Alphaproteobacteria. Within the genus Azospirillum, strain BV-ST was most closely related to Azospirillum doebereinerae GSF71T, A. picis IMMIB TAR-3T and A. lipoferum ATCC 29707T (97.7, 97.7 and 97.4 % 16S rRNA gene sequence similarity, respectively). DNA–DNA relatedness between strain BV-ST and A. doebereinerae DSM 13131T, A. picis DSM 19922T and A. lipoferum ATCC 29707T was 38, 55 and 42 %, respectively. Similarities between nifH sequences of strain BV-ST and members of the genus Azospirillum ranged from 94.5 to 96.8 %. Chemotaxonomic characteristics (quinone Q-10, major fatty acid C18 : 1 ω7c and G+C content 67 mol%) were similar to those of members of the genus Azospirillum. In contrast to known Azospirillum species, strain BV-ST was capable of mixotrophic growth under microaerobic conditions with simultaneous utilization of organic substrates and thiosulfate as electron donors for energy conservation. Oxidation of sulfide was accompanied by deposits of sulfur globules within the cells. Based on these observations, strain BV-ST is considered as a representative of a novel species of the genus Azospirillum, for which the name Azospirillum thiophilum sp. nov. is proposed. The type strain is BV-ST (=DSM 21654T =VKM B-2513T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.018853-0