1887

Abstract

A taxonomic study was carried out on strain F44-8, which was isolated from a crude-oil-degrading consortium, enriched from marine sediment of the Beibu Gulf, PR China. The 16S rRNA gene sequence of strain F44-8 showed highest similarities to those of LMG 21922 (93.3 %), R2A1-13 (93.3 %) and LMG 21477 (93.1 %). Sequence similarities to other members of the genus were <93.0 %. The dominant fatty acids of strain F44-8 were iso-C, summed feature 3 (iso-C 2-OH and/or C 7), iso-C G and iso-C 3-OH. The DNA G+C content of strain F44-8 was 38.6 mol%. These results are consistent with characteristics of members of the genus . Strain F44-8 could, however, be readily distinguished from all known species by a number of phenotypic features. Therefore, according to the phenotypic and 16S rRNA gene sequence data, strain F44-8 represents a novel species in the genus , for which the name sp. nov. is proposed (type strain F44-8 =CCTCC AB 209067 =LMG 25233 =MCCC 1A02877).

Keyword(s): NJ, neighbour-joining
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2011-01-01
2020-01-20
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References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (editors) ( 1995; ). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York. : Wiley.
    [Google Scholar]
  2. Bergey, D. H., Harrison, F. C., Breed, R. S., Hammer, B. W. & Huntoon, F. M. (editors) ( 1923; ). Bergey's Manual of Determinative Bacteriology. Baltimore. : Williams & Wilkins.
    [Google Scholar]
  3. Bernardet, J.-F. & Bowman, J. ( 2006; ). The genus Flavobacterium. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 481–531. Edited by Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K. H. & Stackebrandt, E.. New York. : Springer.
    [Google Scholar]
  4. Bernardet, J.-F. & Nakagawa, Y. ( 2006; ). An introduction to the family Flavobacteriaceae. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 455–480. Edited by Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K. H. & Stackebrandt, E.. New York. : Springer.
    [Google Scholar]
  5. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P. ( 1996; ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef]
    [Google Scholar]
  6. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  7. Dong, X.-Z. & Cai, M.-Y. ( 2001; ). Determinative Manual for Routine Bacteriology. Beijing. : Scientific Press (English translation ).
    [Google Scholar]
  8. Kimura, M. ( 1980; ). A simple model for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  9. Lai, Q., Yuan, J., Gu, L. & Shao, Z. ( 2009; ). Marispirillum indicum gen. nov., sp. nov., isolated from a deep-sea environment. Int J Syst Evol Microbiol 59, 1278–1281.[CrossRef]
    [Google Scholar]
  10. Liu, C. & Shao, Z. ( 2005; ). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55, 1181–1186.[CrossRef]
    [Google Scholar]
  11. Liu, H., Liu, R., Yang, S.-Y., Gao, W.-K., Zhang, C.-X., Zhang, K.-Y. & Lai, R. ( 2008; ). Flavobacterium anhuiense sp. nov., isolated from field soil. Int J Syst Evol Microbiol 58, 756–760.[CrossRef]
    [Google Scholar]
  12. Mandri, T. & Lin, J. ( 2007; ). Isolation and characterization of engine oil degrading indigenous microrganisms in Kwazulu-Natal, South Africa. Afr J Biotechnol 6, 23–27.
    [Google Scholar]
  13. Mesbah, M. & Whitman, W. B. ( 1989; ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr 479, 297–306.[CrossRef]
    [Google Scholar]
  14. Rzhetsky, A. & Nei, M. ( 1992; ). A simple method for estimating and testing minimum evolution trees. Mol Biol Evol 9, 945–967.
    [Google Scholar]
  15. Rzhetsky, A. & Nei, M. ( 1993; ). Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10, 1073–1095.
    [Google Scholar]
  16. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  17. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, Technical Note 101. Newark, DE: MIDI.
  18. Shieh, W. Y., Chen, Y.-W., Chaw, S.-M. & Chiu, H.-H. ( 2003; ). Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 53, 479–484.[CrossRef]
    [Google Scholar]
  19. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  20. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  21. Van Trappen, S., Mergaert, J. & Swings, J. ( 2003; ). Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 53, 1241–1245.[CrossRef]
    [Google Scholar]
  22. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J. ( 2004; ). Flavobacterium degerlachei sp. nov., Flavobacterium frigoris sp. nov. and Flavobacterium micromati sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54, 85–92.[CrossRef]
    [Google Scholar]
  23. Vela, A. I., Fernandez, A., Sánchez-Porro, C., Sierra, E., Mendez, M., Arbelo, M., Ventosa, A., Domínguez, L. & Fernández-Garayzábal, J. F. ( 2007; ). Flavobacterium ceti sp. nov., isolated from beaked whales (Ziphius cavirostris). Int J Syst Evol Microbiol 57, 2604–2608.[CrossRef]
    [Google Scholar]
  24. Weon, H.-Y., Song, M.-H., Son, J.-A., Kim, B.-Y., Kwon, S.-W., Go, S.-J. & Stackebrandt, E. ( 2007; ). Flavobacterium terrae sp. nov. and Flavobacterium cucumis sp. nov., isolated from greenhouse soil. Int J Syst Evol Microbiol 57, 1594–1598.[CrossRef]
    [Google Scholar]
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vol. , part 1, pp. 205 - 209

Transmission electron micrograph of a negatively stained cell of strain F44-8 grown on 216L agar for 24 h at 28 °C. Bar, 0.5 µm.



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