1887

Abstract

A novel aerobic, heterotrophic bacterium, designated BiosLi39, was isolated from the South East Pacific Ocean. Cells were Gram-negative gliding rods forming yellow colonies on marine agar. The isolate was oxidase-, catalase- and alkaline phosphatase-positive and -galactosidase-negative. Strain BiosLi39 grew at 20-37 °C (optimum 30 °C), at pH 7.0–9.0 (optimum pH 8.0) and with 20–60 g NaCl l (optimum 30–50 g NaCl l). The fatty acids (>1 %) comprised iso-C, iso-C G, iso-C, anteiso-C, C G, C, iso-C 2-OH, iso-C G, iso-C, iso-C 3-OH, iso-C 2-OH, iso-C 3-OH, C 2-OH and three unidentified components with equivalent chain lengths of 17.87, 18.10 and 18.71. A significant proportion of the hydroxylated fatty acids are amide-linked. The lipid pattern indicated the presence of phosphatidylethanolamine, two unidentified aminolipids and three unidentified polar lipids. The strain contained menaquinone 7 as the sole respiratory lipoquinone and did not produce flexirubin-type pigments. The G+C content of the genomic DNA was 37.2 mol%. Comparative 16S rRNA gene sequence analysis indicated that strain BiosLi39 was distantly related to all of the representatives of the phylum . Its closest relative was IFO 15994, with which it shared 92.5 % 16S rRNA gene sequence similarity. On the basis of genotypic, phenotypic and chemotaxonomic characteristics, we propose a novel genus and species, gen. nov., sp. nov., with type strain BiosLi39 (=DSM 19307 =CIP 109600 =OOB 398).

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2010-12-01
2020-11-24
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