1887

Abstract

A Gram-positive, non-motile bacterium, designated KSL51201-037, was isolated from Anyang stream, Republic of Korea, and was characterized using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis showed that strain KSL51201-037 belonged to the family of the class and exhibited 96.9 % gene sequence similarity to KSW2-17, 96.0 % to wged11 and 95.9 % to KV-677. The G+C content of the genomic DNA was 72.7 mol%. Strain KSL51201-037 had -2,4-diaminobutyric acid as the diagnostic cell-wall diamino acid, MK-11 and MK-12 as the major menaquinones, anteiso-C (47.8 %) and iso-C (24.0 %) as the major fatty acids and phosphatidylglycerol and three unknown phospholipids as the major polar lipids. On the basis of phenotypic and genotypic properties and phylogenetic distinctiveness, it is suggested that strain KSL51201-037 represents a novel species of a new genus in the family for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is KSL51201-037 (=KEMC 51201-037=JCM 16463).

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2011-01-01
2019-12-15
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Polar lipid distribution of strain KSL51201-037 after two-dimensional TLC. PG, phosphatidylglycerol; PL, unknown phospholipids.

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Neighbour-joining tree based on 16S rRNA gene sequences, showing the phylogenetic relationships between strain KSL51201-037 and all species of the family . [ PDF] 96 KB

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Fatty acid composition of strain KSL51201-037 and KCTC 19176 . [ PDF] 51 KB

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