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Abstract

Two bacterial strains, 5420S-12 and 5420S-16, isolated from air samples, were characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain 5420S-12 was related phylogenetically to FaiI4 (97.4 % sequence similarity) and 25B (97.1 %) and that strain 5420S-16 was closely related to TFB (98.0 %) and 25B (97.2 %). The G+C content of the genomic DNA was 62.2 mol% for strain 5420S-12 and 61.5 mol% for strain 5420S-16. The major fatty acid was C 7. The results of DNA–DNA hybridization and the phenotypic data showed that strains 5420S-12 and 5420S-16 could be distinguished from phylogenetically related species and represent two novel species within the genus , for which the names sp. nov. (type strain 5420S-12 =KACC 12743 =NBRC 106136) and sp. nov. (type strain 5420S-16 =KACC 12744 =NBRC 106137) are proposed. Furthermore, the reclassification of as comb. nov. (type strain TFB =JCM 11936 =KCTC 12101 =IAM 15034 =ATCC BAA-817) is proposed and an emended description of the genus is provided.

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2010-11-01
2021-04-22
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References

  1. Breznak J. A., Costilow R. N. 1994; Physicochemical factors in growth. In Methods for General and Molecular Bacteriology pp 137–154 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  2. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  4. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  5. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  6. Hiraishi A. 1992; Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15:210–213 [CrossRef]
    [Google Scholar]
  7. Kanso S., Patel B. K. C. 2003; Microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface Australian thermal aquifer. Int J Syst Evol Microbiol 53:401–406 [CrossRef]
    [Google Scholar]
  8. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  9. Kwon S. W., Kim J. S., Park I. C., Yoon S. H., Park D. H., Lim C. K., Go S. J. 2003; Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea.. Int J Syst Evol Microbiol 53:21–27 [CrossRef]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  11. MIDI 1999 Sherlock Microbial Identification System, Operating Manual, version 3.0 Newark, DE: MIDI, Inc;
    [Google Scholar]
  12. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Seldin L., Dubnau D. 1985; Deoxyribonucleic acid homology among Bacillus polymyxa , Bacillus macerans , Bacillus azotofixans , and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35:151–154 [CrossRef]
    [Google Scholar]
  15. Skerman V. B. D. 1967 A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  16. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. . In Methods for General and Molecular Bacteriology pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Takeda M., Suzuki I., Koizumi J. I. 2004a; Balneomonas flocculans gen. nov., sp. nov., a new cellulose-producing member of the α -2 subclass of Proteobacteria . Syst Appl Microbiol 27:139–145 [CrossRef]
    [Google Scholar]
  18. Takeda M., Suzuki I., Koizumi J. I. 2004b; Balneimonas flocculans gen. nov., sp. nov., corrig. In Validation of Publication of New Names and New Combinations Previously Effectively Published Outside the IJSEM , List no. 97. Int J Syst Evol Microbiol 54:631–632 [CrossRef]
    [Google Scholar]
  19. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  20. Zhang J., Song F., Xin Y. H., Zhang J., Fang C. 2009; Microvirga guangxiensis sp. nov., a novel alphaproteobacterium from soil, and emended description of the genus Microvirga . Int J Syst Evol Microbiol 59:1997–2001 [CrossRef]
    [Google Scholar]
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