1887

Abstract

Strain ZS314 was isolated from a sandy intertidal sediment sample collected from the coastal area off the Chinese Antarctic Zhongshan Station, east Antarctica (6 ° 22′ 13″ S 7 ° 21′ 41″ E). The cells were Gram-positive, motile, short rods. The temperature range for growth was 0–26 °C and the pH for growth ranged from 5 to 10, with optimum growth occurring within the temperature range 18–23 °C and pH range 6.0–8.0. Growth occurred in the presence of 0–6 % (w/v) NaCl, with optimum growth occurring in the presence of 2–4 % (w/v) NaCl. Strain ZS314 had MK-10 as the major menaquinone and anteiso-C, iso-C and anteiso-C as major fatty acids. The cell-wall peptidoglycan type was B2 with ornithine as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The genomic DNA G+C content was approximately 67 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain ZS314 represents a new lineage in the family . On the basis of the phylogenetic analyses and phenotypic characteristics, a new genus, namely gen. nov., is proposed, harbouring the novel species sp. nov. with the type strain ZS314 (=DSM 22350 =CCTCC AB 209077).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.018754-0
2010-11-01
2019-12-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/11/2535.html?itemId=/content/journal/ijsem/10.1099/ijs.0.018754-0&mimeType=html&fmt=ahah

References

  1. Aizawa, T., Ve, N. B., Kimoto, K., Iwabuchi, N., Sumida, H., Hasegawa, I., Sasaki, S., Tamura, T., Kudo, T., Suzuki, K., Nakajima, M. & Sunairi, M. ( 2007; ). Curtobacterium ammoniigenes sp. nov., an ammonia-producing bacterium isolated from plants inhabiting acidic swamps in actual acid sulfate soil areas of Vietnam. Int J Syst Evol Microbiol 57, 1447–1452.[CrossRef]
    [Google Scholar]
  2. Behrendt, U., Ulrich, A. & Schumann, P. ( 2001; ). Description of Microbacterium foliorum sp. nov. and Microbacterium phyllosphaerae sp. nov., isolated from the phyllosphere of grasses and the surface litter after mulching the sward, and reclassification of Aureobacterium resistens (Funke et al. 1998) as Microbacterium resistens comb. nov. Int J Syst Evol Microbiol 51, 1267–1276.
    [Google Scholar]
  3. Bredholdt, H., Galatenko, O. A., Engelhardt, K., Fjærvik, E., Terekhova, L. P. & Zotchev, S. B. ( 2007; ). Rare actinomycete bacteria from the shallow water sediments of the Trondheim fjord, Norway: isolation, diversity and biological activity. Environ Microbiol 9, 2756–2764.[CrossRef]
    [Google Scholar]
  4. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  5. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow, M. & Minnikin, D. E.. London: Academic Press.
    [Google Scholar]
  6. Dorofeeva, L. V., Krausova, V. I., Evtushenko, L. I. & Tiedje, J. M. ( 2003; ). Agromyces albus sp. nov., isolated from a plant (Androsace sp.). Int J Syst Evol Microbiol 53, 1435–1438.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  8. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  9. Hepperle, D. ( 2004; ). SequentiX alignment editor. Distributed by the author. http://www.sequentix.de.
  10. Jurado, V., Groth, I., Gonzalez, J. M., Laiz, L. & Saiz-Jimenez, C. ( 2005; ). Agromyces salentinus sp. nov. and Agromyces neolithicus sp. nov. Int J Syst Evol Microbiol 55, 153–157.[CrossRef]
    [Google Scholar]
  11. Kämpfer, P., Rainey, F. A., Anderson, M. A., Nurmiaho Lassila, E.-L., Ulrych, U., Busse, H.-J., Weiss, N., Mikkola, R. & Salkinoja-Salonen, M. ( 2000; ). Frigoribacterium faeni gen. nov., sp. nov., a novel psychrophilic genus of the family Microbacteriaceae. Int J Syst Evol Microbiol 50, 355–363.[CrossRef]
    [Google Scholar]
  12. Kates, M. ( 1986; ). Techniques of Lipidology, 2nd edn. Amsterdam. : Elsevier.
    [Google Scholar]
  13. Li, H., Yu, Y., Luo, W., Zeng, Y. & Chen, B. ( 2009; ). Bacterial diversity in surface sediments from the Pacific Arctic Ocean. Extremophiles 13, 233–246.[CrossRef]
    [Google Scholar]
  14. MacKenzie, S. L. ( 1987; ). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70, 151–160.
    [Google Scholar]
  15. Männistö, M. K., Schumann, P., Rainey, F. A., Kämpfer, P., Tsitko, I., Tiirola, M. A. & Salkinoja-Salonen, M. S. ( 2000; ). Subtercola boreus gen. nov., sp. nov. and Subtercola frigoramans sp. nov., two new psychrophilic actinobacteria isolated from boreal groundwater. Int J Syst Evol Microbiol 50, 1731–1739.
    [Google Scholar]
  16. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  17. Park, Y.-H., Suzuki, K., Yim, D.-G., Lee, K.-C., Kim, E., Yoon, J., Kim, S., Kho, Y., Goodfellow, M. & Komagata, K. ( 1993; ). Suprageneric classification of peptidoglycan group B actinomycetes by nucleotide sequencing of 5S ribosomal RNA. Antonie van Leeuwenhoek 64, 307–313.
    [Google Scholar]
  18. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  19. Schleifer, K. H. ( 1985; ). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156.
    [Google Scholar]
  20. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  21. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  22. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  23. Suzuki, K., Sasaki, J., Uramoto, M., Nakase, T. & Komagata, K. ( 1997; ). Cryobacterium psychrophilum gen. nov., sp. nov., nom. rev., comb. nov., an obligately psychrophilic actinomycete to accommodate ‘Curtobacterium psychrophilum’ Inoue and Komagata 1976. Int J Syst Bacteriol 47, 474–478.[CrossRef]
    [Google Scholar]
  24. Takeuchi, M. & Hatano, K. ( 1998; ). Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. in a redefined genus Microbacterium. Int J Syst Bacteriol 48, 739–747.[CrossRef]
    [Google Scholar]
  25. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  26. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  27. Tiago, I., Pires, C., Mendes, V., Morais, P. V., da Costa, M. & Veríssimo, A. ( 2005; ). Microcella putealis gen. nov., sp. nov., a Gram-positive alkaliphilic bacterium isolated from a nonsaline alkaline groundwater. Syst Appl Microbiol 28, 479–487.[CrossRef]
    [Google Scholar]
  28. Tiago, I., Morais, P. V., da Costa, M. S. & Veríssimo, A. ( 2006; ). Microcella alkaliphila sp. nov., a novel member of the family Microbacteriaceae isolated from a nonsaline alkaline groundwater, and emended description of the genus Microcella. Int J Syst Evol Microbiol 56, 2313–2316.[CrossRef]
    [Google Scholar]
  29. Tsukamoto, T., Takeuchi, M., Shida, O., Murata, H. & Shirata, A. ( 2001; ). Proposal of Mycetocola gen. nov. in the family Microbacteriaceae and three new species, Mycetocola saprophilus sp. nov., Mycetocola tolaasinivorans sp. nov. and Mycetocola lacteus sp. nov., isolated from cultivated mushroom, Pleurotus ostreatus. Int J Syst Evol Microbiol 51, 937–944.[CrossRef]
    [Google Scholar]
  30. Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J. (( 1989; ). ). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16, 176–178.
    [Google Scholar]
  31. Yoon, J.-H., Kang, S.-J., Schumann, P. & Oh, T.-K. ( 2006; ). Yonghaparkia alkaliphila gen. nov., sp. nov., a novel member of the family Microbacteriaceae isolated from an alkaline soil. Int J Syst Evol Microbiol 56, 2415–2420.[CrossRef]
    [Google Scholar]
  32. Zgurskaya, H. I., Evtushenko, L. I., Akimov, V. N. & Kalakoutskii, L. V. ( 1993; ). Rathayibacter gen. nov., including the species Rathayibacter rathayi comb. nov., Rathayibacter tritici comb. nov., Rathayibacter iranicus comb. nov., and six strains from annual grasses. Int J Syst Bacteriol 43, 143–149.[CrossRef]
    [Google Scholar]
  33. Zhang, D.-C., Wang, H.-X., Cui, H.-L., Yang, Y., Liu, H.-C., Dong, X.-Z. & Zhou, P.-J. ( 2007a; ). Cryobacterium psychrotolerans sp. nov., a novel psychrotolerant bacterium isolated from the China No. 1 glacier. Int J Syst Evol Microbiol 57, 866–869.[CrossRef]
    [Google Scholar]
  34. Zhang, L., Xu, Z. & Patel, B. K. C. ( 2007b; ). Frondicola australicus gen. nov., sp. nov., isolated from decaying leaf litter from a pine forest. Int J Syst Evol Microbiol 57, 1177–1182.[CrossRef]
    [Google Scholar]
  35. Zhi, X.-Y., Li, W.-J. & Stackebrandt, E. ( 2009; ). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59, 589–608.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.018754-0
Loading
/content/journal/ijsem/10.1099/ijs.0.018754-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error