1887

Abstract

A halophilic archaeon, strain RO1-6, was isolated from a marine solar saltern in eastern China. Cells of strain RO1-6 were pleomorphic and motile and stained Gram-negative. Strain RO1-6 grew well on complex medium and colonies were red-pigmented. It was able to grow at 20–50 °C (optimum 37 °C), in 2.1–5.1 M NaCl (optimum 3.9 M NaCl), in 0.05–0.70 M MgCl (optimum 0.30 M MgCl) and at pH 6.5–8.0 (optimum pH 7.0). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of strain RO1-6 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids that were chromatographically identical to S-DGD-1 and S-DGD. The 16S rRNA gene sequence of strain RO1-6 showed similarities of 96.9 and 96.4 % to those of the type strains of and , respectively, members of the most closely related recognized genera within the family . The DNA G+C content of strain RO1-6 was 61.2 mol%. Phenotypic characterization and phylogenetic analysis revealed that strain RO1-6 is related to and represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is RO1-6 (=CGMCC 1.8711 =JCM 16054).

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2010-10-01
2019-10-14
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Phase-contrast light micrograph of cells of strain RO1-6 . Bar, 5 µm.

IMAGE

Thin-layer chromatograms on Merck silica gel 60 F aluminium-backed thin-layer plates of phospholipids from strain RO1-6 (a) and glycolipids from strain RO1-6 and related strains (b, c). Lanes: 1 and 8, CGMCC 1.2150 ; 2, JCM 14791 ; 3, BZ256 ; 4, 5 and 10, strain RO1-6 ; 6 and 12, JCM 10706 ; 7, ATCC 33170; 9, JCM 13897 ; 11, CGMCC 1.3730 . Circled spots are glycolipids of strain RO1-6 . PG, Phosphatidylglycerol; PGP-Me, phosphatidylglycerol phosphate methyl ester; GL, glycolipid; F, first dimension of TLC; S, second dimension of TLC.

IMAGE

Thin-layer chromatograms on Merck silica gel 60 F aluminium-backed thin-layer plates of phospholipids from strain RO1-6 (a) and glycolipids from strain RO1-6 and related strains (b, c). Lanes: 1 and 8, CGMCC 1.2150 ; 2, JCM 14791 ; 3, BZ256 ; 4, 5 and 10, strain RO1-6 ; 6 and 12, JCM 10706 ; 7, ATCC 33170; 9, JCM 13897 ; 11, CGMCC 1.3730 . Circled spots are glycolipids of strain RO1-6 . PG, Phosphatidylglycerol; PGP-Me, phosphatidylglycerol phosphate methyl ester; GL, glycolipid; F, first dimension of TLC; S, second dimension of TLC.

IMAGE

Thin-layer chromatograms on Merck silica gel 60 F aluminium-backed thin-layer plates of phospholipids from strain RO1-6 (a) and glycolipids from strain RO1-6 and related strains (b, c). Lanes: 1 and 8, CGMCC 1.2150 ; 2, JCM 14791 ; 3, BZ256 ; 4, 5 and 10, strain RO1-6 ; 6 and 12, JCM 10706 ; 7, ATCC 33170; 9, JCM 13897 ; 11, CGMCC 1.3730 . Circled spots are glycolipids of strain RO1-6 . PG, Phosphatidylglycerol; PGP-Me, phosphatidylglycerol phosphate methyl ester; GL, glycolipid; F, first dimension of TLC; S, second dimension of TLC.

IMAGE

Phylogenetic tree derived from maximum-parsimony analysis based on 16S rRNA gene sequences showing the relationship between strain RO1-6 and close relatives within the family . [PDF](39 KB)

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