1887

Abstract

It has been shown previously, based largely on DNA–DNA hybridizations and partial 16S rRNA gene sequencing, that is genotypically heterogeneous and consists of at least two DNA hybridization groups (HGs). In the present study, the taxonomic status of HGs 1 and 2 was reassessed. A panel of 24 reference strains and isolates previously assigned to one of the two HGs in was subjected to (GTG)-PCR fingerprinting; this resulted in the delineation of two (GTG)-PCR clusters in perfect accordance with the respective HG designations. Based on full 16S rRNA gene sequencing of a selection of reference strains, HGs 1 and 2 showed internal sequence similarities of 99.8 and 99.5 %, respectively. Between the two groups, sequence similarities ranged from 98.8 to 99.1 %. Mean DNA–DNA hybridization values of 74.7–99.9 % were obtained within each of the two HGs, whereas cross-hybridizations between members of HG 1 (including ATCC 13337) and HG 2 revealed only 32.7–48.7 % DNA–DNA hybridization. Previously published and new phenotypic data revealed that a combination of malonate assimilation and -glucosidase activity enabled correct assignment of isolates to one of the two HGs. Collectively, taxonomic data from this study confirm that comprises at least two taxa at the species level, of which HG 1 corresponds to because it includes the type strain ATCC 13337. Strains formerly classified as members of HG 2 represent a novel species, for which the name sp. nov. is proposed; ATCC 29927 (=CDC 4510-73 =LMG 24706), the former reference strain of HG 2, is designated the type strain.

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2010-08-01
2019-10-23
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