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Abstract

An extremely halophilic archaeon, strain RO5-8, was isolated from a disused marine solar saltern in China. The cells were pleomorphic and flat. In static liquid medium, cells floated to the surface. Strain RO5-8 stained Gram-negative and colonies were pink-pigmented. It was able to grow at 30–50 °C (optimum 40 °C), at 2.6–4.3 M NaCl (optimum 3.1 M NaCl), at 0.03–0.5 M MgCl (optimum 0.03 M MgCl) and at pH 5.5–7.5 (optimum pH 6.0–6.5). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of strain RO5-8 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and one major glycolipid chromatographically identical to the sulfated mannosyl glucosyl diether S-DGD-1. On the basis of 16S rRNA gene sequence analysis, strain RO5-8 was closely related to three strains of with similarities of 97.3–97.6 %. The DNA G+C content of strain RO5-8 was 62.1 mol%. The DNA–DNA hybridization value between strain RO5-8 and JCM 14081 was 51.6 %. It was concluded that strain RO5-8 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RO5-8 (=CGMCC 1.8712 =JCM 16055).

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2010-08-01
2020-01-27
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vol. , part 8, pp. 1824 - 1827

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Scanning electron micrographs of cells of strain RO5-8 (a–c), showing the gas vesicles driven out of the cells by vacuum.

Phylogenetic tree derived from maximum-parsimony analysis based on 16S rRNA gene sequences showing the relationship between the member of the genus and related genera within the family .

Thin-layer chromatograms on Merck silica gel 60 F254 aluminium-backed thin-layer plates of the phospholipids (a) and glycolipids (b) from strain RO5-8 and related strains.



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