1887

Abstract

An extremely halophilic archaeon, strain RO5-8, was isolated from a disused marine solar saltern in China. The cells were pleomorphic and flat. In static liquid medium, cells floated to the surface. Strain RO5-8 stained Gram-negative and colonies were pink-pigmented. It was able to grow at 30–50 °C (optimum 40 °C), at 2.6–4.3 M NaCl (optimum 3.1 M NaCl), at 0.03–0.5 M MgCl (optimum 0.03 M MgCl) and at pH 5.5–7.5 (optimum pH 6.0–6.5). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of strain RO5-8 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and one major glycolipid chromatographically identical to the sulfated mannosyl glucosyl diether S-DGD-1. On the basis of 16S rRNA gene sequence analysis, strain RO5-8 was closely related to three strains of with similarities of 97.3–97.6 %. The DNA G+C content of strain RO5-8 was 62.1 mol%. The DNA–DNA hybridization value between strain RO5-8 and JCM 14081 was 51.6 %. It was concluded that strain RO5-8 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RO5-8 (=CGMCC 1.8712 =JCM 16055).

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2010-08-01
2021-03-07
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References

  1. Bardavid R., Mana L., Oren A. 2007; Haloplanus natans gen. nov., sp. nov., an extremely halophilic, gas-vacuolate archaeon isolated from Dead Sea–Red Sea water mixtures in experimental outdoor ponds. Int J Syst Evol Microbiol 57:780–783 [CrossRef]
    [Google Scholar]
  2. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  3. Cui H.-L., Lin Z.-Y., Dong Y., Zhou P.-J., Liu S.-J. 2007; Halorubrum litoreum sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol 57:2204–2206 [CrossRef]
    [Google Scholar]
  4. Cui H.-L., Zhou P.-J., Oren A., Liu S.-J. 2009 Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium . Extremophiles 13, 31–37 [CrossRef]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  6. Dussault H. P. 1955; An improved technique for staining red halophilic bacteria. J Bacteriol 70:484–485
    [Google Scholar]
  7. Dyall-Smith M. L. 2008; The Halohandbook: protocols for haloarchaeal genetics. http://www.haloarchaea.com/resources/halohandbook
    [Google Scholar]
  8. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  9. Gonzalez C., Gutierrez C., Ramirez C. 1978; Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24:710–715 [CrossRef]
    [Google Scholar]
  10. Gutiérrez C., González C. 1972; Method for simultaneous detection of proteinase and esterase activities in extremely halophilic bacteria. Appl Microbiol 24:516–517
    [Google Scholar]
  11. Gutiérrez M. C., Castillo A. M., Kamekura M., Ventosa A. 2008; Haloterrigena salina sp. nov., an extremely halophilic archaeon isolated from a salt lake. Int J Syst Evol Microbiol 58:2880–2884 [CrossRef]
    [Google Scholar]
  12. He M.-Y., Zou Z.-Z., Cai L., Wang G.-J. 2008; Polymorphic halophilic bacteria and haloarchaea from Lianyungang Taibei and Yancheng Sanwei salt field soils. Microbiology China) 35:737–742
    [Google Scholar]
  13. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  14. Kates M. 1986 In Techniques of Lipidology , 2nd revised edn, pp. 106–107, 187–188 and 251–254 Amsterdam: Elsevier;
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  16. Ng W. L., Yang C. F., Halladay J. T., Arora A., DasSarma S. 1995; Protocol 25. Isolation of genomic and plasmid DNAs from Halobacterium halobium . In Archaea: a Laboratory Manual: Halophiles pp 179–180 Edited by DasSarma S., Fleischmann E. M. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Oren A., Ventosa A., Grant W. D. 1997; Proposed minimal standards for description of new taxa in the order Halobacteriales . Int J Syst Bacteriol 47:233–238 [CrossRef]
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  19. Sørensen K. B., Canfield D. E., Teske A. P., Oren A. 2005; Community composition of a hypersaline endoevaporitic microbial mat. Appl Environ Microbiol 71:7352–7365 [CrossRef]
    [Google Scholar]
  20. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  21. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  22. Vaskovsky V. E., Kostetsk E. Y. 1968; Modified spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res 9: 396
    [Google Scholar]
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