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Abstract

A novel halophilic, filamentous actinomycete strain, designated YIM 93221, was isolated from a salt lake in Xinjiang province, north-west China, and subjected to a polyphasic taxonomic characterization. The isolate grew with 9–23 % (w/v) NaCl and did not grow without NaCl. The isolate formed spiny aerial mycelium and did not form spores at maturity. The isolate contained -diaminopimelic acid as the diagnostic diamino acid and glucose, glucosamine, mannose and an unknown sugar as the major whole-cell sugars. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and an unknown phospholipid. MK-8(H) was the predominant menaquinone. The major fatty acid was iso-C. The DNA G+C content was 68.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 93221 formed a distinct lineage within the suborder and showed 91.9–94.8 % 16S rRNA gene sequence similarity with members of the suborder . On the basis of the evidence from this polyphasic study, a novel genus and species, gen. nov., sp. nov., are proposed. The type strain of is YIM 93221 (=DSM 45207 =CCTCC AB 208140).

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2010-09-01
2020-01-29
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Scanning electron micrograph of spiny aerial mycelium of strain YIM 93221 grown on oatmeal agar (15% NaCl, w/v) at 37 °C for 4 weeks. Bar, 2 µm.

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Chromatogram of polar lipids of strain YIM 93221 grown in ISP 4 (15% NaCl, w/v). [PDF](39 KB)

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