1887

Abstract

Phenotypic and phylogenetic studies were performed on three strains of an acetogenic bacterium isolated from livestock-impacted soil. The bacterium stained Gram-negative and was a non-spore-forming rod that was motile by peritrichous flagella. The novel strains had an optimum pH for growth of 8.0–8.5 and utilized H : CO, CO : CO, glucose, fructose, mannose, turanose, ribose, trimethylamine, pyruvate, methanol, ethanol, -propanol and -butanol as growth substrates. Acetate was produced from glucose. Acetate, CO and ethanol were produced from CO : CO. 16S rRNA gene sequence analysis indicated that the novel strains formed a new subline in the family (rRNA cluster XV) of the low G+C-containing Gram-positive bacteria of the class . The DNA G+C base composition was 34 mol%. Cell wall analysis revealed the existence of a novel B-type peptidoglycan similar to the B2-type (B4) configuration with a variation containing aspartic acid. Based on phylogenetic and phenotypic evidence, it is proposed that the new isolates represent a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is CP11 (=ATCC BAA-1772=DSM 22112).

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2010-10-01
2019-10-16
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Transmission electron micrograph of cells of CP11 . Bar, 1µm.



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