1887

Abstract

A Gram-staining-negative, yellow-coloured, strictly aerobic, non-spore-forming, rod-shaped bacterium, designated HS39, isolated from a soil sample collected from a natural forest in Xinjiang, China, was characterized using a polyphasic approach. The isolate grew optimally at 30–37 °C, at pH 6.5–8.0 and with 0–3 % NaCl. Analysis of the 16S rRNA gene sequence of strain HS39 revealed that it is a member of the genus . ATCC 33299 was the nearest relative (94.0 % 16S rRNA gene sequence similarity). The G+C content of the genomic DNA was 40.2 mol%. The major fatty acids were iso-C, iso-C 3-OH and summed feature 3 (comprising C 6 and/or C 7). The predominant isoprenoid quinone was MK-7. On the basis of phenotypic properties and phylogenetic inference, strain HS39 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HS39 (=CCTCC AB 209006 =NRRL B-59203).

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2010-10-01
2019-10-22
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References

  1. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  2. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  5. Kim, K.-H., Ten, L. N., Liu, Q.-M., Im, W.-T. & Lee, S.-T. ( 2006; ). Sphingobacterium daejeonense sp. nov., isolated from a compost sample. Int J Syst Evol Microbiol 56, 2031–2036.[CrossRef]
    [Google Scholar]
  6. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  7. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge. : Cambridge University Press.
    [Google Scholar]
  8. Liu, R., Liu, H., Zhang, C. X., Yang, S. Y., Liu, X. H., Zhang, K. Y. & Lai, R. ( 2008; ). Sphingobacterium siyangense sp. nov., isolated from farm soil. Int J Syst Evol Microbiol 58, 1458–1462.[CrossRef]
    [Google Scholar]
  9. Matsuyama, H., Katoh, H., Ohkushi, T., Satoh, A., Kawahara, K. & Yumoto, I. ( 2008; ). Sphingobacterium kitahiroshimense sp. nov., isolated from soil. Int J Syst Evol Microbiol 58, 1576–1579.[CrossRef]
    [Google Scholar]
  10. Mehnaz, S., Weselowski, B. & Lazarovits, G. ( 2007; ). Sphingobacterium canadense sp. nov., an isolate from corn roots. Syst Appl Microbiol 30, 519–524.[CrossRef]
    [Google Scholar]
  11. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  12. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  13. Shivaji, S., Ray, M. K., Rao, N. S., Saisree, L., Jagannadham, M. V., Kumar, G. S., Reddy, G. S. N. & Bhargava, P. M. ( 1992; ). Sphingobacterium antarcticus sp. nov., a psychrotrophic bacterium from the soils of Schirmacher Oasis, Antarctica. Int J Syst Bacteriol 42, 102–106.[CrossRef]
    [Google Scholar]
  14. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  15. Steyn, P. L., Segers, P., Vancanneyt, M., Sandra, P., Kersters, K. & Joubert, J. J. ( 1998; ). Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. Proposal of the family Sphingobacteriaceae fam. nov. Int J Syst Bacteriol 48, 165–177.[CrossRef]
    [Google Scholar]
  16. Takeuchi, M. & Yokota, A. ( 1992; ). Proposals of Sphingobacterium faecium sp. nov., Sphingobacterium piscium sp. nov., Sphingobacterium heparinum comb. nov., Sphingobacterium thalpophilum comb. nov., and two genospecies of the genus Sphingobacterium and synonymy of Flavobacterium yabuuchiae and Sphingobacterium spiritivorum. J Gen Appl Microbiol 38, 465–482.[CrossRef]
    [Google Scholar]
  17. Ten, L. N., Liu, Q.-M., Im, W.-T., Aslam, Z. & Lee, S.-T. ( 2006; ). Sphingobacterium composti sp. nov., a novel DNase-producing bacterium isolated from compost. J Microbiol Biotechnol 16, 1728–1733.
    [Google Scholar]
  18. Wei, W., Zhou, Y., Wang, X., Huang, X. & Lai, R. ( 2008; ). Sphingobacterium anhuiense sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 58, 2098–2101.[CrossRef]
    [Google Scholar]
  19. Wilson, K. ( 1987; ). Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Edited by Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K.. New York. : Green Publishing & Wiley-Interscience.
    [Google Scholar]
  20. Xie, C. H. & Yokota, A. ( 2003; ). Phylogenetic analysis of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49, 345–349.[CrossRef]
    [Google Scholar]
  21. Yabuuchi, E., Kaneko, T., Yano, I., Moss, C. W. & Miyoshi, N. ( 1983; ). Sphingobacterium gen. nov., Sphingobacterium spiritivorum comb. nov., Sphingobacterium multivorum comb. nov., Sphingobacterium mizutae sp. nov., and Flavobacterium indologenes sp. nov.: glucose-nonfermenting Gram-negative rods in CDC groups IIK-2 and IIb. Int J Syst Bacteriol 33, 580–598.[CrossRef]
    [Google Scholar]
  22. Yoo, S. H., Weon, H. Y., Jang, H. B., Kim, B. Y., Kwon, S. W., Go, S. J. & Stackebrandt, E. ( 2007; ). Sphingobacterium composti sp. nov., isolated from cotton-waste composts. Int J Syst Evol Microbiol 57, 1590–1593.[CrossRef]
    [Google Scholar]
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