1887

Abstract

Three housekeeping genes (, and ) of strains belonging to the genus (37 strains) or related taxa (38 strains) were sequenced. Reference strains of the 15 species of the genus were included. Phylogenetic trees generated using these gene sequences confirmed the existence of two phylogenetic groups within the genus . These groups clustered separately in trees constructed using concatenated sequences of the three genes, indicating that the genus should not remain a single genus and should be split, as suggested previously. Multilocus sequence analysis (MLSA) of the three housekeeping genes also proved useful for species differentiation in the family . It also suggested that LMG 18788, better known as the type and only strain of subsp. , represents a distinct species in the genus , and is not a true strain. In previous studies, this strain showed less than 70 % DNA relatedness to the type strains of and , the phylogenetically nearest relatives, and could be distinguished from them phenotypically. Additionally, AFLP and (GTG)-PCR DNA fingerprinting data supported its reclassification within a distinct species. The name (Toyosaki 1996) sp. nov., comb. nov. is proposed.

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2010-10-01
2024-03-28
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