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Volume 60, Issue 9

gen. nov., sp. nov., a halophilic actinobacterium, and proposal of fam. nov. Free

Abstract

A Gram-staining-positive, facultatively anaerobic, non-motile and moderately halophilic actinobacterium, designated YIM 93306, was isolated from a salt lake in Xinjiang province, north-west China, and subjected to a polyphasic taxonomic study. Strain YIM 93306 grew in the presence of 2–16 % (w/v) NaCl and did not grow without NaCl. The peptidoglycan type was A4 with an -Lys–-Glu interpeptide bridge. The whole-cell sugars were glucosamine, arabinose, mannose and two unknown sugars. The predominant menaquinone was MK-8(H). The major fatty acids were iso-C, anteiso-C and anteiso-C. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phosphoglycolipid and one unknown phospholipid. The DNA G+C content was 68.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 93306 fell within the radius of the suborder . Its closest phylogenetic neighbour was the type strain of (AS 4.3142; 96.2 % 16S rRNA gene sequence similarity), the sole recognized species of the genus . Sequence similarities between strain YIM 93306 and members of other genera of the suborder were <95.2 %. On the basis of phylogenetic analysis, phenotypic characteristics and chemotaxonomic differences, a novel genus and species, gen. nov., sp. nov., is proposed. The type strain of the species is YIM 93306 (=DSM 21368 =KCTC 19413 =CCTCC AB 208069). Based on phylogenetic characteristics and 16S rRNA gene signature nucleotide patterns, the genera and gen. nov. are proposed to belong to a novel family, fam. nov.

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2010-09-01
2024-04-19
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Haloactinobacterium album gen. nov., sp. nov., a halophilic actinobacterium, and proposal of Ruaniaceae fam. nov.
Int J Syst Evol Microbiol 60, 2113 (2010); https://doi.org/10.1099/ijs.0.018440-0
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