1887

Abstract

Three bacterial isolates from air samples in Korea, designated strains 6424S-25, 6515J-31 and 6424S-61, were characterized using a polyphasic approach. The cells were strictly aerobic, Gram-stain-negative, non-motile, non-spore-forming and rod-shaped. Phylogenetic analysis of their 16S rRNA gene sequences revealed a clear affiliation with the phylum . Strains 6424S-25 and 6515J-31 showed 16S rRNA gene sequence similarities of 92.7–94.8 % to type strains of recognized species of the genus and strain 6424S-61 was closely related to Gsoil 664 (93.9 % similarity). The G+C contents of the DNA of strains 6424S-25, 6515J-31 and 6424S-61 were 44.5, 43.9 and 38.4 mol%, respectively. Major fatty acids of strains 6424S-25 and 6515J-31 were summed feature 4 (iso-C I and/or anteiso-C B), iso-C and C 5. The fatty acid content of strain 6424S-61 mainly comprised iso-C G and iso-C. Comparative analysis of phenotypic and phylogenetic traits indicated that strains 6424S-25 and 6515J-31 represented two novel species of the genus and that strain 6424S-61 should be considered as a novel species of the genus . The names sp. nov. (type strain 6424S-25 =KACC 14118 =NBRC 106134), sp. nov. (type strain 6515J-31 =KACC 14117 =NBRC 106133) and sp. nov. (type strain 6424S-61 =KACC 14119 =NBRC 106135) are proposed for these organisms.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.018374-0
2010-10-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/10/2424.html?itemId=/content/journal/ijsem/10.1099/ijs.0.018374-0&mimeType=html&fmt=ahah

References

  1. An, D.-S., Lee, H.-G., Im, W.-T., Liu, Q.-M. & Lee, S.-T. ( 2007; ). Segetibacter koreensis gen. nov., sp. nov., a novel member of the phylum Bacteroidetes, isolated from the soil of a ginseng field in South Korea. Int J Syst Evol Microbiol 57, 1828–1833.[CrossRef]
    [Google Scholar]
  2. Breznak, J. A. & Costilow, R. N. ( 1994; ). Physicochemical factors in growth. In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  3. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y.-W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  5. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  6. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  7. Hiraishi, A. ( 1992; ). Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15, 210–213.[CrossRef]
    [Google Scholar]
  8. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  9. Kwon, S. W., Kim, J. S., Park, I. C., Yoon, S. H., Park, D. H., Lim, C. K. & Go, S. J. ( 2003; ). Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53, 21–27.[CrossRef]
    [Google Scholar]
  10. Ludwig, W., Euzéby, J. & Whitman, W. B. ( 2008; ). Draft taxonomic outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi, and Gemmatimonadetes. http://www.bergeys.org/outlines/Bergeys_Vol_4_Outline.pdf.
  11. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  12. MIDI ( 1999; ). Sherlock Microbial Identification System Operating Manual, version 3.0 . Newark, DE. : MIDI, Inc.
    [Google Scholar]
  13. Rickard, A. H., Stead, A. T., O'May, G. A., Lindsay, S., Banner, M., Handley, P. S. & Gilbert, P. ( 2005; ). Adhaeribacter aquaticus gen. nov., sp. nov., a Gram-negative isolate from a potable water biofilm. Int J Syst Evol Microbiol 55, 821–829.[CrossRef]
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  16. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  17. Ten, L. N., Baek, S.-H., Im, W.-T., Liu, Q.-M., Aslam, Z. & Lee, S.-T. ( 2006; ). Bacillus panaciterrae sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 56, 2861–2866.[CrossRef]
    [Google Scholar]
  18. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  19. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  20. Zhang, J.-Y., Liu, X.-Y. & Liu, S.-J. ( 2009; ). Adhaeribacter terreus sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 59, 1595–1598.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.018374-0
Loading
/content/journal/ijsem/10.1099/ijs.0.018374-0
Loading

Data & Media loading...

Supplements

vol. , part 10, pp. 2424 - 2429

Supplementary data [ PDF] (71 KB) containing:

Cellular fatty acid compositions of strains 6424S-25 and 6515J-31 and the type strains of species of the genus .

Fatty acid profiles of strains 6424S-61 and Gsoil 664 .

Differential carbon source utilization between strains 6424S-25 and 6515J-31 .



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error