1887

Abstract

A polyphasic taxonomic study of a halotolerant bacterium, isolated from sandy rhizospheric soil in Sarbandar, Persian Gulf, Iran, revealed that strain HM6 represents a novel species within the genus . Results of the 16S rRNA gene sequence comparison revealed that strain HM6 clustered with strains of the genus , showing the highest degree of 16S rRNA gene sequence similarity to (99.2 %), (98.5 %) and (98.3 %). However, DNA–DNA hybridization studies with these type strains revealed less than 39.6 % similarity. Rather than genotypic differences, there are some phenotypic discrepancies between strain HM6 and closely related species of the genus . Main morphological and chemotaxonomical features of strain HM6 include: (i) growth characteristics, i.e. the formation of a scant light-yellow to white aerial mycelium and the typical zig-zag form of the hyphae, which fragment during ageing into smooth rod-shaped spores; (ii) the presence of -diaminopimelic acid and glucose plus ribose in whole-cell hydrolysates; (iii) the presence of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol, together with three unknown -specific phospholipids (close to diphosphatidylglycerol in position) in polar lipid extracts; (iv) the presence of the major menaquinones MK-10(H), MK-10(H) and MK-9(H) in the non-polar fraction; (v) the presence of iso/anteiso-branched plus 10-methyl-branched fatty acids, showing the diagnostic combination for species of the genus of iso-16 : 0 (31.1 %), anteiso-17 : 0 (19.2 %), 10-methyl-17 : 0 (5.8 %) and tuberculostearic acid (8.8 %); and (vi) the absence of mycolic acids. Analysis of the 16S rRNA gene sequence revealed that strain HM6 represents a distinct taxon within the genus . Based upon genotypic and phenotypic differences from other members of the genus, a novel species, sp. nov., is proposed. The type strain is HM6 (=UTMC 00102 =DSM 45277 =CCUG 57624).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.018366-0
2010-10-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/10/2346.html?itemId=/content/journal/ijsem/10.1099/ijs.0.018366-0&mimeType=html&fmt=ahah

References

  1. Al-Tai A. M., Ruan J. S. 1994; Nocardiopsis halophila sp. nov., a new halophilic actinomycete isolated from soil. Int J Syst Bacteriol 44:474–478 [CrossRef]
    [Google Scholar]
  2. Al-Zarban S. S., Abbas I., Al-Musallam A. A., Steiner U., Stackebrandt E., Kroppenstedt R. M. 2002; Nocardiopsis halotolerans sp. nov., isolated from salt marsh soil in Kuwait. Int J Syst Evol Microbiol 52:525–529
    [Google Scholar]
  3. Brocq-Rousseau D. 1904; Sur un Streptothrix . Ref Gen Botanique 16:219–230
    [Google Scholar]
  4. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  5. Chen Y.-G., Cui X.-L., Kroppenstedt R. M., Stackebrandt E., Wen M.-L., Xu L.-H., Jiang C.-L. 2008; Nocardiopsis quinghaiensis sp. nov., isolated from saline soil in China. Int J Syst Evol Microbiol 58:699–705 [CrossRef]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  7. Evtushenko L. I., Taran V. V., Akimov V. N., Kroppenstedt R. M., Tiedje J. M., Stackebrandt E. 2000; Nocardiopsis tropica sp. nov., Nocardiopsis trehalosi sp. nov., nom. rev. and Nocardiopsisdassonvillei subsp. albirubida subsp. nov., comb. nov. Int J Syst Evol Microbiol 50:73–81 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1993 phylip (phylogeny inference package) version 3.5.1. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  9. Fitch W. M. 1972; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416
    [Google Scholar]
  10. Galatenko O. A., Trekhova L. P. 1990; Isolation of antibiotic producing actinomycetes from soil samples exposed to UV light. Antibiot Khimioter 35:6–8 (in Russian)
    [Google Scholar]
  11. Hayakawa M., Kajiura T., Nonomura H. 1991; New Methods for the highly selective isolation of Streptosporangium and Dactylosporangium from soil. J Ferment Bioeng 72:327–333 [CrossRef]
    [Google Scholar]
  12. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  13. Kawato N., Shinobu R. 1959; On Streptomyces herbaricolor sp. nov., supplement: a single technique for microscopical observation. Mem Osaka Univ Lib Arts Educ B 8:114–119
    [Google Scholar]
  14. Kelly K. L. 1964 Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  15. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2367 [CrossRef]
    [Google Scholar]
  16. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol 20) pp 173–199 Edited by Goodfellow M., Minnikin D. E. New York: Academic Press;
    [Google Scholar]
  17. Kroppenstedt R. M. 1992; The genus Nocardiopsis . In The Prokaryotes, 2nd edn. pp 1139–1156 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  18. Kroppenstedt R. M., Evtushenko L. I. 2006; The family Nocardiopsaceae . In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn. vol 3 pp 754–795 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E.
    [Google Scholar]
  19. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy (Special Publication no. 6) pp 227–291 Edited by Dietz A., Thayer D. W. Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  20. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  21. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  22. Meyer J. 1976; Nocardiopsis , a new genus of the order Actinomycetales . Int J Syst Bacteriol 26:487–493 [CrossRef]
    [Google Scholar]
  23. Meyer J. 1989; Genus Nocardiopsis Meyer 1976, 487AL . In Bergey's Manual of Systematic Bacteriology vol 4 pp 2562–2569 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  24. Mikami Y., Miyashita K., Arai T. 1982; Diaminopimelic acid profiles of alkalophilic and alkaline-resistant strains of actinomycetes. J Gen Microbiol 128:1709–1712
    [Google Scholar]
  25. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  26. Mishra S. K., Keller J. E., Miller J. R., Heisey R. M., Nair M. G., Putnam A. R. 1987a; Insecticidal and nematicidal properties of microbial metabolites. J Ind Microbiol 2:267–276 [CrossRef]
    [Google Scholar]
  27. Mishra S. K., Traft W. H., Putnam A. R., Ries S. K. 1987b; Plant growth regulatory metabolites from novel actinomycetes. J Plant Growth Regul 6:75–84 [CrossRef]
    [Google Scholar]
  28. Nolan R. D., Cross T. 1988; Isolation and screening of actinomycetes. In Actinomycetes in Biotechnology pp 2–8 Edited by Goodfellow M., Williams S. T., Mordarski M. London: Academic Press;
    [Google Scholar]
  29. Nonomura H., Ohara Y. 1971a; Distribution of actinomycetes in soil. X. New genus and species of monosporic actinomycetes. J Ferment Technol 49:895–903
    [Google Scholar]
  30. Nonomura H., Ohara Y. 1971b; Distribution of actinomycetes in soil. XI. Some new species of the genus Actinomadura Lechevalier et al . J Ferment Technol 49:904–912
    [Google Scholar]
  31. Peltola J. S. P., Anderson M. A., Kämpfer P., Auling G., Kroppenstedt R. M., Busse H.-J., Salkinoja-Salonen M. S., Rainey F. A. 2001; Isolation of toxigenic Nocardiopsis strains from indoor environments and description of two new Nocardiopsis species; N. exhalans sp. nov. and N. umidischolae sp. nov. Appl Environ Microbiol 67:4293–4304 [CrossRef]
    [Google Scholar]
  32. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  33. Sabry S. A., Ghanem N. B., Abu-Ella G. A., Schumann P., Stackebrandt E., Kroppenstedt R. M. 2004; Nocardiopsis aegyptia sp. nov., isolated from marine sediment. Int J Syst Microbiol 54:453–456 [CrossRef]
    [Google Scholar]
  34. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  35. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  36. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  37. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
    [Google Scholar]
  38. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  39. Staneck J. L., Roberts G. D. 1974; Simplified approach to the identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  40. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  41. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  42. Williams S. T., Shameemullah M., Watson E. T., Mayfield C. I. 1972; Studies on the ecology of actinomycetes in soil. VI. The influence of moisture tension on growth and survival. Soil Biol Biochem 4:215–225 [CrossRef]
    [Google Scholar]
  43. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related taxa. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  44. Yassin A. F., Galinski E. A., Wohlfarth A., Jahnke K.-D., Schaal K. P., Trüper H. G. 1993; A new actinomycete species, Nocardiopsis lucentensis sp. nov. Int J Syst Bacteriol 43:266–271 [CrossRef]
    [Google Scholar]
  45. Yassin A. F., Rainey F. A., Burghardt J., Gierth D., Ungerechts J., Lux I., Seifert P., Bal C., Schaal K. P. 1997; Description of Nocardiopsis synnemataformans sp. nov., elevation of Nocardiopsis alba subsp. prasina to Nocardiopsis prasina comb. nov., and designation of Nocardiopsis antarctica and Nocardiopsis alborubida as later subjective synonyms of Nocardiopsis dassonvillei . Int J Syst Bacteriol 47:983–988 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.018366-0
Loading
/content/journal/ijsem/10.1099/ijs.0.018366-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error