1887

Abstract

A polyphasic taxonomic study of a halotolerant bacterium, isolated from sandy rhizospheric soil in Sarbandar, Persian Gulf, Iran, revealed that strain HM6 represents a novel species within the genus . Results of the 16S rRNA gene sequence comparison revealed that strain HM6 clustered with strains of the genus , showing the highest degree of 16S rRNA gene sequence similarity to (99.2 %), (98.5 %) and (98.3 %). However, DNA–DNA hybridization studies with these type strains revealed less than 39.6 % similarity. Rather than genotypic differences, there are some phenotypic discrepancies between strain HM6 and closely related species of the genus . Main morphological and chemotaxonomical features of strain HM6 include: (i) growth characteristics, i.e. the formation of a scant light-yellow to white aerial mycelium and the typical zig-zag form of the hyphae, which fragment during ageing into smooth rod-shaped spores; (ii) the presence of -diaminopimelic acid and glucose plus ribose in whole-cell hydrolysates; (iii) the presence of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol, together with three unknown -specific phospholipids (close to diphosphatidylglycerol in position) in polar lipid extracts; (iv) the presence of the major menaquinones MK-10(H), MK-10(H) and MK-9(H) in the non-polar fraction; (v) the presence of iso/anteiso-branched plus 10-methyl-branched fatty acids, showing the diagnostic combination for species of the genus of iso-16 : 0 (31.1 %), anteiso-17 : 0 (19.2 %), 10-methyl-17 : 0 (5.8 %) and tuberculostearic acid (8.8 %); and (vi) the absence of mycolic acids. Analysis of the 16S rRNA gene sequence revealed that strain HM6 represents a distinct taxon within the genus . Based upon genotypic and phenotypic differences from other members of the genus, a novel species, sp. nov., is proposed. The type strain is HM6 (=UTMC 00102 =DSM 45277 =CCUG 57624).

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2010-10-01
2020-01-24
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