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Abstract

Two novel strains of Gram-negative, non-sporulating, short rod-shaped, motile bacteria, designated DCY25 and DCY28, were isolated from soil of a ginseng field in South Korea and characterized in order to determine their taxonomic position. 16S rRNA gene sequence analysis showed that strains DCY25 and DCY28 belonged to the , the highest sequence similarities being found with BN9 (96.9 %), DSM 11334 (95.9 %), DSM 30026 (95.9 %), LMG 6003 (95.8 %) and K24 (95.5 %). Chemotaxonomic data revealed that both strains DCY25 and DCY28 possessed ubiquinone Q-8. Fatty acid analysis of strain DCY25 demonstrated the presence of 19 : 0 cyclo 8 (22.8 %) and 16 : 0 (16.6 %). The polar lipid profiles of strains DCY25 and DCY28 included phosphatidylethanolamine, phosphatidylglycerol, two unknown aminolipids and diphosphatidylglycerol. The G+C contents of strains DCY25 and DCY28 were 57.3 and 57.2 mol%, respectively. DNA–DNA relatedness values, biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiation of strains DCY25 and DCY28 from . Therefore, strains DCY25 and DCY28 should be classified in a novel species, for which the name sp. nov. is proposed. The type strain is DCY25 (=KCTC 22046 =JCM 14767); strain DCY28 (=KCTC 22047=JCM 14768) is a reference strain.

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2010-08-01
2019-08-18
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References

  1. Blümel, S., Mark, B., Busse, H.-J., Kämpfer, P. & Stolz, A. ( 2001; ). Pigmentiphaga kullae gen. nov., sp. nov., a novel member of the family Alcaligenaceae with the ability to decolorize azo dyes aerobically. Int J Syst Evol Microbiol 51, 1867–1871.[CrossRef]
    [Google Scholar]
  2. Buck, J. D. ( 1982; ). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993.
    [Google Scholar]
  3. Cappuccino, J. G. & Sherman, N. ( 2002; ). Microbiology: a Laboratory Manual, 6th edn. Menlo Park, CA: Benjamin/Cummings.
  4. Collins, M. D. & Jones, D. ( 1981; ). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45, 316–354.
    [Google Scholar]
  5. De Ley, J., Segers, P., Kersters, K., Mannheim, W. & Lievens, A. ( 1986; ). Intra- and intergeneric similarities of the Bordetella ribosomal ribonucleic acid cistrons: proposal for a new family, Alcaligenaceae. Int J Syst Bacteriol 36, 405–414.[CrossRef]
    [Google Scholar]
  6. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  9. Kim, M. K., Im, W.-T., Ohta, H., Lee, M. & Lee, S.-T. ( 2005; ). Sphingopyxis granuli sp. nov., a beta-glucosidase-producing bacterium in the family Sphingomonadaceae in alpha-4 subclass of the Proteobacteria. J Microbiol 43, 152–157.
    [Google Scholar]
  10. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  11. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  13. Minnikin, D. E., Patel, P. V., Alshamaony, L. & Goodfellow, M. ( 1977; ). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27, 104–117.[CrossRef]
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  16. Shin, Y. K., Lee, J.-S., Chun, C. O., Kim, H.-J. & Park, Y.-H. ( 1996; ). Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036T. J Microbiol Biotechnol 6, 68–69.
    [Google Scholar]
  17. Stolz, A., Bürger, S., Kuhm, A., Kämpfer, P. & Busse, H.-J. ( 2005; ). Pusillimonas noertemannii gen. nov., sp. nov., a new member of the family Alcaligenaceae that degrades substituted salicylates. Int J Syst Evol Microbiol 55, 1077–1081.[CrossRef]
    [Google Scholar]
  18. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  19. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  20. von Wintzingerode, F., Schattke, A., Siddiqui, R. A., Rösick, U., Göbel, U. B. & Gross, R. ( 2001; ). Bordetella petrii sp. nov., isolated from an anaerobic bioreactor, and emended description of the genus Bordetella. Int J Syst Evol Microbiol 51, 1257–1265.
    [Google Scholar]
  21. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
  22. Yabuuchi, E. & Yano, I. ( 1981; ). Achromobacter gen. nov. and Achromobacter xylosoxidans (ex Yabuuchi and Ohyama 1971) nom. rev. Int J Syst Bacteriol 31, 477–478.[CrossRef]
    [Google Scholar]
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vol. , part 8, pp. 1783 - 1787

Two-dimensional TLC of polar lipids of strain DCY25 visualized with 5 % ethanolic molybdatophosphoric acid. DPG, Diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; AL, unknown amino lipid; APL, unknown aminophospholipid; L, unknown glycolipid.

DNA–DNA relatedness between strains DCY25 and DCY28 and the type strains of the type species of closely related genera

Standard deviations were in the range 1–4 %.



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