1887

Abstract

A bacterial consortium able to degrade the herbicide 4-(2,4-dichlorophenoxy) butyric acid (2,4-DB) was obtained from an agricultural soil of the Argentinean Humid Pampa region which has a history of long-term herbicide use. Four bacterial strains were isolated from the consortium and identified as members of the genera , and A polyphasic systematic analysis was carried out on strain CPDB6, the member of the 2,4-DB-degrading consortium able to degrade 2,4-DB as a sole carbon and energy source. The Gram-negative, rod-shaped, motile, non-sporulating, non-fermenting bacterium was shown to belong to the genus on the basis of 16S rRNA gene sequence analyses. Strain CPDB6 did not reduce nitrate, which differentiated it from the type species of the genus, ; it did not grow in 0.5–4.5 % NaCl, although most species of are able to grow at NaCl concentrations as high as 1.5 %; and it was able to deamidate acetamide, which differentiated it from all other species of . DNA–DNA hybridization data revealed low levels of genomic DNA similarity (less than 30 %) between strain CPDB6 and the type strains of species with validly published names The major cellular fatty acids detected were -9-hexadecenoic (16 : 17) and hexadecanoic (16 : 0) acids. On the basis of phenotypic and genotypic characterizations, strain CPDB6 was recognized as a representative of a novel species within the genus . The name sp. nov. is proposed, with strain CPDB6 (=CCUG 55948=CCM-A-29:1289) as the type strain.

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2010-11-01
2019-10-21
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References

  1. Chen, W.-M., Laevens, S., Lee, T.-M., Coenye, T., De Vos, P., Mergeay, M. & Vandamme, P. ( 2001; ). Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int J Syst Evol Microbiol 51, 1729–1735.[CrossRef]
    [Google Scholar]
  2. Coenye, T., Falsen, E., Vancanneyt, M., Hoste, B., Govan, J. R. W., Kersters, K. & Vandamme, P. ( 1999; ). Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49, 405–413.[CrossRef]
    [Google Scholar]
  3. Coenye, T., Goris, J., De Vos, P., Vandamme, P. & LiPuma, J. J. ( 2003; ). Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov. Int J Syst Evol Microbiol 53, 1075–1080.[CrossRef]
    [Google Scholar]
  4. Cuadrado, V., Merini, L. J., Flocco, C. G. & Giulietti, A. M. ( 2008; ). Degradation of 2,4-DB in Argentinean agricultural soils with high humic matter content. Appl Microbiol Biotechnol 77, 1371–1378.[CrossRef]
    [Google Scholar]
  5. EWG ( 2006; ). National Tap Water Quality Database. Environmental Working Group. (http://ewg.org. )
  6. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1989; ). phylip – phylogeny inference package (version 3.2). Cladistics 5, 164–166.
    [Google Scholar]
  8. Goris, J., De Vos, P., Coenye, T., Hoste, B., Janssens, D., Brim, H., Diels, L., Mergeay, M., Kersters, K. & Vandamme, P. ( 2001; ). Classification of metal-resistant bacteria from industrial biotopes as Ralstonia campinensis sp. nov., Ralstonia metallidurans sp. nov., and Ralstonia basilensis Steinle et al. 1998 emend. Int J Syst Evol Microbiol 51, 1773–1782.[CrossRef]
    [Google Scholar]
  9. Gosselin, R. E., Smith, R. P. & Hodge, H. C. ( 1984; ). Clinical Toxicology of Commercial Products, 5th edn. Baltimore. : Williams and Wilkins.
    [Google Scholar]
  10. Hauben, L., Vauterin, L., Swings, J. & Moore, E. R. B. ( 1997; ). Comparison of 16S ribosomal DNA sequences of all Xanthomonas species. Int J Syst Bacteriol 47, 328–335.[CrossRef]
    [Google Scholar]
  11. Hernández, M., Villalobos, P., Morgante, V., González, M., Reiff, C., Moore, E. & Seeger, M. ( 2008; ). Isolation and characterization of a novel simazine-degrading bacterium from agricultural soil of central Chile, Pseudomonas sp. MHP41. FEMS Microbiol Lett 286, 184–190.[CrossRef]
    [Google Scholar]
  12. HSDB ( 2005; ). Hazardous Substances Data Bank. US National Library of Medicine. (http://toxnet.nlm.nih.gov/. )
  13. IARC ( 2003; ). International Agency for Research on Cancer Monographs. World Health Organization.
  14. INDEC ( 2002; ). Censo Nacional Agropecuario. Buenos Aires, Argentina. : Instituto Nacional de Estadísticas y Censos.
    [Google Scholar]
  15. Johannesen, H. & Aamand, J. ( 2003; ). Mineralization of aged atrazine, terbuthylazine, 2,4-D, and mecoprop in soil and aquifer sediment. Environ Toxicol Chem 22, 722–729.[CrossRef]
    [Google Scholar]
  16. Makkar, N. S. & Casida, L. E., Jr ( 1987; ). Cupriavidus necator gen. nov., sp. nov.: a nonobligate bacterial predator of bacteria in soil. Int J Syst Bacteriol 37, 323–326.[CrossRef]
    [Google Scholar]
  17. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  18. Merini, L. J., Cuadrado, V., Flocco, C. G. & Giulietti, A. M. ( 2007; ). Dissipation of 2,4-D in soils of the Humid Pampa region, Argentina: a microcosm study. Chemosphere 68, 259–265.[CrossRef]
    [Google Scholar]
  19. Merini, L. J., Cuadrado, V. & Giulietti, A. M. ( 2008; ). Spiking solvent, humidity and their impact on 2,4-D and 2,4-DCP extractability from high humic matter content soils. Chemosphere 71, 2168–2172.[CrossRef]
    [Google Scholar]
  20. Page, R. D. M. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  21. Pearson, W. R. & Lipman, D. J. ( 1988; ). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, 2444–2448.[CrossRef]
    [Google Scholar]
  22. Reasoner, D. J. & Geldreich, E. E. ( 1985; ). A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49, 1–7.
    [Google Scholar]
  23. Sahin, N., Isik, K., Tamer, A. U. & Goodfellow, M. ( 2000; ). Taxonomic position of ‘‘Pseudomonas oxalaticus’’ strain Ox1T (DSM 1105T) (Khambata and Bhat, 1953) and its description in the genus Ralstonia as Ralstonia oxalatica comb. nov. Syst Appl Microbiol 23, 206–209.[CrossRef]
    [Google Scholar]
  24. Sato, Y., Nishihara, H., Yoshida, M., Watanabe, M., Rondal, J. D., Concepcion, R. N. & Ohta, H. ( 2006; ). Cupriavidus pinatubonensis sp. nov. and Cupriavidus laharis sp. nov., novel hydrogen-oxidizing, facultatively chemolithotrophic bacteria isolated from volcanic mudflow deposits from Mt. Pinatubo in the Philippines. Int J Syst Evol Microbiol 56, 973–978.[CrossRef]
    [Google Scholar]
  25. Schop, R. N., Hardy, M. H. & Goldberg, M. T. ( 1990; ). Comparison of the activity of topically applied pesticides and the herbicide 2,4-D in two short-term in vivo assays of genotoxicity in the mouse. Fundam Appl Toxicol 15, 666–675.[CrossRef]
    [Google Scholar]
  26. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  27. Steinle, P., Stucki, G., Stettler, R. & Hanselmann, K. W. ( 1998; ). Aerobic mineralization of 2,6-dichlorophenol by Ralstonia sp. Strain RK1. Appl Environ Microbiol 64, 2566–2571.
    [Google Scholar]
  28. Steinle, P., Stucki, G., Stettler, R. & Hanselmann, K. W. ( 1999; ). Ralstonia basilensis sp. nov. In Validation of publication of new names and new combinations previously effectively published outside the IJSB, List no. 71. Int J Syst Bacteriol 49, 1325–1326.[CrossRef]
    [Google Scholar]
  29. Stevens, J. T. & Sumner, D. D. ( 1991; ). Herbicides. In Handbook of Pesticide Toxicology, vol. 3, pp. 1317–1408. Edited by W. J., Hayes, Jr & E. R., Laws, Jr. New York, NY. : Academic Press.
    [Google Scholar]
  30. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  31. Urdiain, M., López-López, A., Gonzalo, C., Busse, H. J., Langer, S., Kämpfer, P. & Roselló-Móra, R. ( 2008; ). Reclassification of Rhodobium marinum and Rhodobium pfennigii as Afifella marina gen. nov. comb. nov. and Afifella pfennigii comb. nov., a new genus of photoheterotrophic Alphaproteobacteria and emended descriptions of Rhodobium, Rhodobium orientis and Rhodobium gokarnense. Syst Appl Microbiol 31, 339–351.[CrossRef]
    [Google Scholar]
  32. US EPA ( 1992; ). Pesticides in Ground Water Database: a Compilation of Monitoring Studies: 1971–1991 National Summary. Washington, DC. : US Environmental Protection Agency.
    [Google Scholar]
  33. Vandamme, P. & Coenye, T. ( 2004; ). Taxonomy of the genus Cupriavidus: a tale of lost and found. Int J Syst Evol Microbiol 54, 2285–2289.[CrossRef]
    [Google Scholar]
  34. Vandamme, P., Goris, J., Coenye, T., Hoste, B., Janssens, D., Kersters, K., De Vos, P. & Falsen, E. ( 1999; ). Assignment of Centers for Disease Control group IVc-2 to the genus Ralstonia as Ralstonia paucula sp. nov. Int J Syst Bacteriol 49, 663–669.[CrossRef]
    [Google Scholar]
  35. Vaneechoutte, M., Kämpfer, P., De Baere, T., Falsen, E. & Verschraegen, G. ( 2004; ). Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov. Int J Syst Evol Microbiol 54, 317–327.[CrossRef]
    [Google Scholar]
  36. Walker, M. M. & Keith, L. H. ( 1992; ). EPA's Pesticide Fact Sheet Database. Chelsea, MI. : Lewis Publishers.
    [Google Scholar]
  37. Wauters, G., Claeys, G., Verschraegen, G., De Baere, T., Vandecruys, E., Van Simaey, L., De Ganck, C. & Vaneechoutte, M. ( 2001; ). Case of catheter sepsis with Ralstonia gilardii in a child with acute lymphoblastic leukemia. J Clin Microbiol 39, 4583–4584.[CrossRef]
    [Google Scholar]
  38. Weed Science Society of America ( 1994; ). Herbicide Handbook, 7th edn. Champaign, IL. : Weed Science Society of America.
    [Google Scholar]
  39. Yabuuchi, E., Kosako, Y., Yano, I., Hotta, H. & Nishiuchi, Y. ( 1995; ). Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov.: proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. nov., Ralstonia solanacearum (Smith 1896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov. Microbiol Immunol 39, 897–904.[CrossRef]
    [Google Scholar]
  40. Zahm, S. H., Weisenburger, D. D., Babbitt, P. A., Saal, R. C., Vaught, J. B., Cantor, K. P. & Blair, A. ( 1990; ). A case-control study of non-Hodgkin's lymphoma and the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) in eastern Nebraska. Epidemiology 1, 349–356.[CrossRef]
    [Google Scholar]
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