Burkholderia acidipaludis sp. nov., aluminium-tolerant bacteria isolated from Chinese water chestnut (Eleocharis dulcis) growing in highly acidic swamps in South-East Asia
Two strains of aluminium-tolerant bacteria, SA33T and 7A078, were isolated from Chinese water chestnut (Eleocharis dulcis) growing in highly acidic swamps (pH 2–4) in actual acid sulfate soil areas of Vietnam (SA33T) and Thailand (7A078). The strains were Gram-negative, aerobic, non-spore-forming rods, 0.6–0.7 μm wide and 1.3–1.7 μm long. These strains showed good growth at pH 3.0–8.0 and 17–37 °C. The organisms contained ubiquinone Q-8 as the predominant isoprenoid quinone and C16 : 0, C18 : 1ω7c and C17 : 0 cyclo as the major fatty acids. Their fatty acid profiles were similar to those reported for other Burkholderia species. The DNA G+C content of these strains was 64 mol%. On the basis of 16S rRNA gene sequence similarity, the strains were shown to belong to the genus Burkholderia. Although the 16S rRNA gene sequence similarity values calculated for strain SA33T to 7A078 and the type strains of Burkholderia kururiensis, B. sacchari and B. tuberum were 100, 97.3, 97.1 and 97.0 %, respectively, strains SA33T and 7A078 formed a group that was distinct in the phylogenetic trees; the DNA–DNA relatedness of strain SA33T to 7A078 and these three type strains were respectively 90, 47, 46 and 45 %. The results of physiological and biochemical tests, including whole-cell protein pattern analysis, allowed phenotypic differentiation of these strains from described Burkholderia species. Therefore, strains SA33T and 7A078 represent a novel species, for which the name Burkholderia acidipaludis sp. nov. is proposed. The type strain is SA33T (=NBRC 101816T =VTCC-D6-6T). Strain 7A078 (=NBRC 103872 =BCC 36999) is a reference strain.
AizawaT.,
NguyenB. V.,
VijarnsornP.,
KimotoK.,
SasakiS.,
NakajimaM.,
SunairiM.2008; Application of symbiotic bacteria isolated from plants adapted to actual acid sulfate soil. In Development of New Bioremediation Systems of Acid Sulfate Soil for Agriculture and Forestry pp 57–62 Edited by
SasakiS. & other authors. Kyoto: Shoukadoh;
AizawaT.,
NguyenB. V.,
NakajimaM.,
SunairiM.2010; Burkholderia heleia sp. nov., a nitrogen-fixing bacterium isolated from an aquatic plant, Eleocharis dulcis , that grows in highly acidic swamps in actual acid sulfate soil areas of Vietnam. Int J Syst Evol Microbiol 60:1152–1157[CrossRef]
BaldaniV. L. D.,
OliveiraE.,
BalotaE.,
BaldaniJ. I.,
KirchhofG.,
DöbereinerJ.1997; Burkholderia brasilensis sp. nov., uma nova espécie de bactéria diazotrófica endofitica. An Acad Bras Cienc 69: 116 (in Portuguese
BrämerC. O.,
VandammeP.,
da SilvaL. F.,
GomezJ. G. C.,
SteinbüchelA.2001; Burkholderia sacchari sp. nov., a polyhydroxyalkanoate-accumulating bacterium isolated from soil of a sugar-cane plantation in Brazil. Int J Syst Evol Microbiol 51:1709–1713[CrossRef]
Caballero-MelladoJ.,
Onofre-LemusJ.,
Estrada-de los SantosP.,
Martínez-AguilarL.2007; The tomato rhizosphere, an environment rich in nitrogen-fixing Burkholderia species with capabilities of interest for agriculture and bioremediation. Appl Environ Microbiol 73:5308–5319[CrossRef]
ChenW. M.,
JamesE. K.,
CoenyeT.,
ChouJ. H.,
BarriosE.,
de FariaS. M.,
ElliottG. N.,
SheuS. Y.,
SprentJ. I.,
VandammeP.2006; Burkholderia mimosarum sp. nov., isolated from root nodules of Mimosa spp. from Taiwan and South America. Int J Syst Evol Microbiol 56:1847–1851[CrossRef]
ChenW.-M.,
de FariaS. M.,
JamesE. K.,
ElliottG. N.,
LinK.-Y.,
ChouJ.-H.,
SheuS.-Y.,
CnockaertM.,
SprentJ. I.,
VandammeP.2007; Burkholderia nodosa sp. nov. isolated from root nodules of the woody Brazilian legumes Mimosa bimucronata and Mimosa scabrella
. Int J Syst Evol Microbiol 57:1055–1059[CrossRef]
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229[CrossRef]
FelsensteinJ.2005phylip (Phylogeny Inference Package) version 3.65. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
HashidokoY.,
TadaM.,
OsakiM.,
TaharaS.2002; Soft gel medium solidified with gellan gum for preliminary screening for root-associating, free-living nitrogen-fixing bacteria inhabiting the rhizoplane of plants. Biosci Biotechnol Biochem 66:2259–2263[CrossRef]
JukesT. H.,
CantorC. R.1969; Evolution of protein molecules. In Mammalian Protein Metabolismvol 3 pp 21–132 Edited by
MunroH. N.
New York: Academic Press;
KimotoK.,
AizawaT.,
UraiM.,
NguyenB. V.,
SuzukiK.,
NakajimaM.,
SunairiM.2010; Acidocella aluminiidurans sp. nov., an aluminium-tolerant bacterium isolated from Panicum repens grown in a highly acidic swamp in actual acid sulfate soil area of Vietnam. Int J Syst Evol Microbiol 60:764–768[CrossRef]
PerinL.,
Martinez-AguilarL.,
Paredes-ValdezG.,
BaldaniJ. I.,
Estrada-de los SantosP.,
ReisV. M.,
Caballero-MelladoJ.2006; Burkholderia silvatlantica sp. nov., a diazotrophic bacterium associated with sugar cane and maize. Int J Syst Evol Microbiol 56:1931–1937[CrossRef]
PotB.,
VandammeP.,
KerstersK.1994; Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics (Modern Microbial Methods Series) pp 493–521 Edited by
GoodfellowM.,
O'DonnellA. G.
Chichester: Wiley;
SasakiS.,
IshiiR.,
HasegawaI.,
TokuyamaT.,
HanzawaK.,
SumidaH.,
UedaS.,
NoguchiA.,
MatsumotoR.other authors2008Development of New Bioremediation Systems of Acid Sulfate Soil for Agriculture and Forestry Kyoto: Shoukadoh;
SmibertR. M.,
KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology. pp 607–654 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
StackebrandtE.,
GoebelB.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849[CrossRef]
TamuraT.,
HatanoK.2001; Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. , 1986 and ‘Actinoplanes aurantiacus' to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol 51:2119–2125[CrossRef]
TamuraT.,
NakagaitoY.,
NishiiT.,
HasegawaT.,
StackebrandtE.,
YokotaA.1994 A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb.nov. and Couchioplanescaeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44, 193–203 [CrossRef]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.other authors1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]
WeberO. B.,
BaldaniV. L. D.,
TeixeiraK. R. S.,
KirchhofG.,
BaldaniJ. I.,
DöbereinerJ.1999; Isolation and characterization of diazotrophic bacteria from banana and pineapple plants. Plant Soil 210:103–113[CrossRef]
YabuuchiE.,
KosakoY.,
OyaizuH.,
YanoI.,
HottaH.,
HashimotoY.,
EzakiT.,
ArakawaM.1992; Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol Immunol 36:1251–1275[CrossRef]
YamadaY.,
Takinami-NakamuraH.,
TaharaY.,
OyaizuH.,
KomagataK.1982; The ubiquinone systems in the strains of Pseudomonas species. J Gen Appl Microbiol 28:7–12[CrossRef]
YangH. C.,
ImW. T.,
KimK. K.,
AnD. S.,
LeeS. T.2006; Burkholderia terrae sp. nov., isolated from a forest soil. Int J Syst Evol Microbiol 56:453–457[CrossRef]
Burkholderia acidipaludis sp. nov., aluminium-tolerant bacteria isolated from Chinese water chestnut (Eleocharis dulcis) growing in highly acidic swamps in South-East Asia