1887

Abstract

A novel Gram-negative, aerobic, motile, short rod-shaped bacterium, designated MS-3, was isolated from a crude oil-contaminated seashore in Taean, Korea. Strain MS-3 grew at 4–30 °C, at pH 6.0–9.5 and with 0–5 % NaCl and was oxidase- and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MS-3 was most similar to KMM 3042 (97.9 % 16S rRNA gene sequence similarity), 1N (97.8 %), R-20821 (97.3 %) and ATCC 49968 (97.1 %). Relatively low levels of DNA–DNA relatedness were found between strain MS-3 and LMG 24676 (57.2 %), LMG 23199 (39.7 %), KMM 3042 (32.2 %) and KACC 10832 (32.1 %), which support the classification of strain MS-3 within a novel species of the genus . The G+C content of the genomic DNA of strain MS-3 was 57.6 mol% and the major isoprenoid quinone was Q-9. Strain MS-3 contained summed feature 3 (iso-C 2-OH and/or C 7; 38.0 %), C (24.4 %), C 7 (12.8 %), C (9.6 %) and C 3-OH (4.9 %) as the major cellular fatty acids. On the basis of the phenotypic, genotypic and phylogenetic data, strain MS-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MS-3 (=KCTC 22612 =KACC 14032 =JCM 16046 =NBRL 105641).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.018093-0
2010-12-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/12/2719.html?itemId=/content/journal/ijsem/10.1099/ijs.0.018093-0&mimeType=html&fmt=ahah

References

  1. Anzai Y., Kim H., Park J.-Y., Wakabayashi H., Oyaizu H. 2000; Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50:1563–1589 [CrossRef]
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  3. Bhattacharya D., Sarma P. M., Krishnan S., Mishra S., Lal B. 2003; Evaluation of genetic diversity among Pseudomonas citronellolis strains isolated from oily sludge-contaminated sites. Appl Environ Microbiol 69:1435–1441 [CrossRef]
    [Google Scholar]
  4. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
    [Google Scholar]
  5. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  6. Cole J. R., Chai B., Marsh T. L., Farris R. J., Wang Q., Kulam S. A., Chandra S., McGarrell D. M., Schmidt T. M. other authors 2003; The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31:442–443 [CrossRef]
    [Google Scholar]
  7. Elkin S., Geddes D. 2003; Pseudomonal infection in cystic fibrosis: the battle continues. Expert Rev Anti Infect Ther 1:609–618 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  9. Gupta S. K., Rekha Kumari R., Om Prakash O., Lal R. 2008; Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site. Int J Syst Evol Microbiol 58:1339–1345 [CrossRef]
    [Google Scholar]
  10. Hansen G. H., Sørheim R. 1991; Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 13:231–241 [CrossRef]
    [Google Scholar]
  11. Johnsen K., Andersen S., Jacobsen C. S. 1996; Phenotypic and genotypic characterization of phenanthrene-degrading fluorescent Pseudomonas biovars. Appl Environ Microbiol 62:3818–3825
    [Google Scholar]
  12. Kahng H.-Y., Nam K., Kukor J. J., Yoon B.-J., Lee D.-H., Oh D.-C., Kam S.-K., Oh K.-H. 2002; PAH utilization by Pseudomonas rhodesiae KK1 isolated from a former manufactured-gas plant site. Appl Microbiol Biotechnol 60:475–480 [CrossRef]
    [Google Scholar]
  13. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;
    [Google Scholar]
  14. King E. O., Ward M. K., Rainey D. E. 1954; Two simple media for the demonstration of pyocyanin and fluorescein. J Lab Clin Med 44:301–307
    [Google Scholar]
  15. Kiyohara H., Takizawa N., Nagao K. 1992; Natural distribution of bacteria metabolizing many kinds of polycyclic aromatic hydrocarbons. J Ferment Bioeng 74:49–51 [CrossRef]
    [Google Scholar]
  16. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  17. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  18. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175 Edited by Stackebrandt E., Goodfellow M. New York: Wiley;
    [Google Scholar]
  19. Levitski-Heikkila T. V., Ullian M. E. 2005; Peritonitis with multiple rare environmental bacteria in a patient receiving long-term peritoneal dialysis. Am J Kidney Dis 46:e119–e124 [CrossRef]
    [Google Scholar]
  20. López-Romalde S., Magarinõs B., Ravelo C., Toranzo A. E., Romalde J. L. 2003; Existence of two O-serotypes in the fish pathogen Pseudomonas anguilliseptica . Vet Microbiol 94:325–333 [CrossRef]
    [Google Scholar]
  21. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  22. Mikesell M. D., Kukor J. J., Olsen R. H. 1993; Metabolic diversity of aromatic hydrocarbon-degrading bacteria from a petroleum-contaminated aquifer. Biodegradation 4:249–259
    [Google Scholar]
  23. Mishra S., Lal B., Jyot J., Rajan S., Khanna S. 1999; Field study: in situ bioremediation of oily sludge contaminated land using oilzapper. In Proceedings of Hazardous and Industrial Wastes Symposium pp 177–186 Edited by Bishop D. Lancaster, PA: Technomic Publishing Co;
    [Google Scholar]
  24. Mishra S., Jyot J., Kuhad R. C., Lal B. 2001; In situ bioremediation potential of an oily sludge-degrading bacterial consortium. Curr Microbiol 43:328–335 [CrossRef]
    [Google Scholar]
  25. O'Mahony M. M., Dobson A. D., Barnes J. D., Singleton I. 2006; The use of ozone in the remediation of polycyclic aromatic hydrocarbon contaminated soil. Chemosphere 63:307–314 [CrossRef]
    [Google Scholar]
  26. Onaca C., Kieninger M., Engesser K.-H., Altenbuchner J. 2007; Degradation of alkyl methyl ketones by Pseudomonas veronii MEK700. J Bacteriol 189:3759–3767 [CrossRef]
    [Google Scholar]
  27. Oyaizu H., Komagata K. 1983; Grouping of Pseudomonas species on the basis of cellular fatty acid composition and the quinone system with special reference to the existence of 3-hydroxy fatty acids. J Gen Appl Microbiol 29:17–40 [CrossRef]
    [Google Scholar]
  28. Palleroni N. J. 1984; Genus I. Pseudomonas Migula 1984, 237AL . In Bergey's Manual of Systematic Bacteriology vol 1 pp 141–199 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  29. Palleroni N. J. 1993; Pseudomonas classification. A new case history in the taxonomy of Gram-negative bacteria. Antonie van Leeuwenhoek 64:231–251
    [Google Scholar]
  30. Prakash O., Kumari K., Lal R. 2007; Pseudomonas delhiensis sp. nov., from a fly ash dumping site of a thermal power plant. Int J Syst Evol Microbiol 57:527–531 [CrossRef]
    [Google Scholar]
  31. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  32. Sneath P. H. A., Stevens M., Sackin M. J. 1981; Numerical taxonomy of Pseudomonas based on published records of substrate utilization. Antonie van Leeuwenhoek 47:423–448 [CrossRef]
    [Google Scholar]
  33. Stolz A., Busse H.-J., Kämpfer P. 2007; Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57:572–576 [CrossRef]
    [Google Scholar]
  34. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  35. Vancanneyt M., Segers P., Torck U., Hoste B., Bernardet J.-F., Vandamme P., Kersters K. 1996; Reclassification of Flavobacterium odoraturn (Stutzer 1929) strains to a new genus, Myroides , as Myroides odoratus comb.nov. and Myroides odoratimimus sp. nov.. Int J Syst Bacteriol 46:926–932 [CrossRef]
    [Google Scholar]
  36. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  37. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  38. Whyte L. G., Goalen B., Hawari J., Labbe D., Greer C. W., Nahir M. 2001; Bioremediation treatability assessment of hydrocarbon-contaminated soils from Eureka, Nunavut. Cold Reg Sci Technol 32:121–132 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.018093-0
Loading
/content/journal/ijsem/10.1099/ijs.0.018093-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error