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Volume 60, Issue 8

gen. nov., sp. nov., isolated from compost, description of fam. nov. within the class Cavaletti 2007 and emended description of the class Free

Abstract

We isolated from compost an aerobic, thermophilic, Gram-stain-positive, spore-forming bacterium that formed branched vegetative and aerial mycelia. This strain, designated SK20-1, grew at 31–58 °C, with optimum growth at 50 °C, while no growth was observed below 28 or above 60 °C. The pH range for growth was 5.4–8.7, with optimum growth at pH 7.0, while no growth was observed below pH 5.0 or above pH 9.1. Strain SK20-1 was able to hydrolyse polysaccharides such as cellulose, xylan and chitin. The DNA G+C content was 54.0 mol%. The major fatty acid was iso-C and the major menaquinone was MK-9(H). The cell wall contained glutamic acid, serine, alanine and ornithine in a molar ratio of 1.00 : 1.07 : 2.64 : 0.83. The polar lipids consisted of phosphatidylinositol, phosphatidylinositol mannosides, phosphatidylglycerol, diphosphatidylglycerol and an unknown glycolipid. Cell-wall sugars were rhamnose and mannose. Detailed phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SK20-1 belongs to the class , and that the strain is most closely related to SOSP1-21 (88.5 %). On the basis of its phenotypic features and phylogenetic position, we propose that SK20-1 represents a novel genus and species, gen. nov., sp. nov., within the new family fam. nov. The type strain of is strain SK20-1 (=JCM 16142 =ATCC BAA-1881). In addition, we propose an emended description of the class to classify the class in the phylum .

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2010-08-01
2024-09-14
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Thermosporothrix hazakensis gen. nov., sp. nov., isolated from compost, description of Thermosporotrichaceae fam. nov. within the class Ktedonobacteria Cavaletti et al. 2007 and emended description of the class Ktedonobacteria
Int J Syst Evol Microbiol 60, 1794 (2010); https://doi.org/10.1099/ijs.0.018069-0
/content/journal/ijsem/10.1099/ijs.0.018069-0
/content/journal/ijsem/10.1099/ijs.0.018069-0
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